##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	511951
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16147053135945	33.0	31.0	34.0	30.0	34.0
2	32.29779412482835	34.0	31.0	34.0	30.0	34.0
3	32.34547056261244	34.0	31.0	34.0	30.0	34.0
4	35.873472265900446	37.0	35.0	37.0	35.0	37.0
5	35.783168701692155	37.0	35.0	37.0	35.0	37.0
6	35.76398131852462	37.0	35.0	37.0	35.0	37.0
7	35.83667577561134	37.0	35.0	37.0	35.0	37.0
8	35.72907172756768	37.0	35.0	37.0	35.0	37.0
9	37.588110971557825	39.0	37.0	39.0	35.0	39.0
10	37.114645737580354	39.0	37.0	39.0	33.0	39.0
11	37.03421811853087	39.0	37.0	39.0	33.0	39.0
12	36.90212930534368	39.0	35.0	39.0	33.0	39.0
13	36.79448619106125	39.0	37.0	39.0	33.0	39.0
14	37.98625063726802	40.0	37.0	41.0	33.0	41.0
15	38.097136249367615	40.0	37.0	41.0	33.0	41.0
16	38.18725424894179	40.0	37.0	41.0	34.0	41.0
17	38.09900947551621	40.0	37.0	41.0	33.0	41.0
18	38.04301583549988	40.0	37.0	41.0	33.0	41.0
19	37.94353365849466	40.0	36.0	41.0	34.0	41.0
20	37.85266558713627	40.0	36.0	41.0	33.0	41.0
21	37.75125939787206	40.0	35.0	41.0	33.0	41.0
22	37.710450804862184	40.0	35.0	41.0	33.0	41.0
23	37.62193061445333	40.0	35.0	41.0	33.0	41.0
24	37.58411840195644	39.0	35.0	41.0	33.0	41.0
25	37.49676433877461	39.0	35.0	41.0	33.0	41.0
26	37.390037327791134	39.0	35.0	41.0	33.0	41.0
27	37.361335362173335	39.0	35.0	41.0	33.0	41.0
28	37.28320288465107	39.0	35.0	41.0	32.0	41.0
29	37.18807854657965	39.0	35.0	41.0	32.0	41.0
30	37.07764414953775	39.0	35.0	41.0	32.0	41.0
31	36.9612638709564	39.0	35.0	41.0	31.0	41.0
32	36.84401241525068	39.0	35.0	41.0	31.0	41.0
33	36.731628612894596	39.0	35.0	41.0	31.0	41.0
34	36.62670060220607	39.0	35.0	41.0	31.0	41.0
35	36.483079435336585	39.0	35.0	41.0	30.0	41.0
36	36.37348300911611	39.0	35.0	41.0	30.0	41.0
37	36.29152594681913	39.0	35.0	41.0	30.0	41.0
38	36.130918779336305	38.0	35.0	40.0	30.0	41.0
39	36.08271885395282	38.0	35.0	40.0	30.0	41.0
40	35.94193975595321	38.0	35.0	40.0	29.0	41.0
41	35.800205488415884	38.0	35.0	40.0	29.0	41.0
42	35.67401763059355	38.0	35.0	40.0	29.0	41.0
43	35.57431472933933	38.0	35.0	40.0	28.0	41.0
44	35.43608665673082	38.0	34.0	40.0	28.0	41.0
45	35.33560047738944	37.0	34.0	40.0	27.0	41.0
46	35.21058851335382	37.0	34.0	40.0	27.0	41.0
47	35.09339761031818	37.0	34.0	40.0	27.0	41.0
48	34.935388347712966	37.0	34.0	40.0	26.0	41.0
49	34.85681051506882	37.0	34.0	40.0	26.0	41.0
50	34.77589456803483	36.0	34.0	40.0	26.0	41.0
51	33.40233537975314	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	3.0
12	8.0
13	11.0
14	22.0
15	31.0
16	78.0
17	130.0
18	211.0
19	442.0
20	602.0
21	1004.0
22	1422.0
23	2075.0
24	2816.0
25	3677.0
26	4700.0
27	5623.0
28	6549.0
29	7850.0
30	9682.0
31	12107.0
32	15460.0
33	21049.0
34	36617.0
35	49055.0
36	42929.0
37	60806.0
38	96966.0
39	129976.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08439674890761	20.234358366328028	22.84925705780436	13.831987826960003
2	29.727063722895352	25.763989131772373	26.98246511873207	17.526482026600203
3	31.10219532728718	27.71144113401478	25.455756507947047	15.73060703075099
4	27.118220298426998	27.52665782467463	26.90101201091511	18.454109865983266
5	22.079066160628656	33.601262620836756	24.812335555551215	19.507335662983373
6	22.984035581530264	37.64852495648998	24.78205922051134	14.585380241468421
7	79.24742797650556	9.02977042724792	7.988850495457574	3.7339511007889423
8	81.05717148711497	6.997544686893862	7.979474598154901	3.9658092278362576
9	76.20963725043998	10.001152454043453	8.93132350556987	4.857886789946694
10	38.716205261831696	27.417858349724877	16.53654353639313	17.329392852050294
11	30.740637287552907	28.218130250746654	21.53272481155423	19.508507650146207
12	26.23356532168118	24.264431556926347	29.42000308623286	20.082000035159613
13	21.923191867971738	28.85940256001063	29.479774431537393	19.737631140480243
14	20.219122533211188	30.55761195895701	28.738101888657315	20.48516361917449
15	19.455182234237263	28.267158380391873	31.48856042863477	20.789098956736094
16	21.413377452138974	23.88724702168762	32.151514500411174	22.54786102576223
17	20.73889883992804	25.217061789116535	27.594633080118996	26.44940629083643
18	23.272735085975025	25.77942029608302	27.965176354768328	22.982668263173625
19	24.624817609497786	28.777949452193667	24.500782301431194	22.096450636877357
20	24.923479004826635	26.740254438413054	25.23268828462099	23.103578272139323
21	25.03735709081533	27.59326576176236	25.850520850628282	21.518856296794027
22	22.489652330008145	24.571687524782647	26.030030217735682	26.908629927473527
23	21.42978527241865	29.466882572746222	25.131311395035855	23.97202075979928
24	21.70012364464568	27.32312272072913	29.450474752466544	21.526278882158646
25	23.741920613496216	27.144590009590765	26.224970749153727	22.888518627759296
26	20.155639895224347	27.981584175048003	27.249287529470596	24.613488400257054
27	22.09059070106319	27.321755402372496	27.51083599797637	23.07681789858795
28	20.4923908733453	28.51698697726931	28.511127041455136	22.47949510793025
29	21.863615853861017	28.992227771798472	26.840654672029157	22.303501702311355
30	23.144207160450904	26.949844809366517	27.66143634840053	22.244511681782043
31	24.976413758347967	28.633209037583672	23.77668956599362	22.613687638074737
32	24.18375977388461	27.923180148100112	25.874937249853993	22.01812282816129
33	24.191182359249225	27.96556701715594	23.399114368367286	24.44413625522755
34	21.97495463433024	28.253094534437867	26.192350439788182	23.579600391443712
35	21.867131815349516	28.44276112362316	25.48681416776215	24.20329289326518
36	24.43241638359921	28.31677250361851	26.014989715812646	21.23582139696963
37	24.017728259149802	28.005023918304683	25.74133071329092	22.235917109254597
38	22.96352580618067	29.008635592078146	25.562016677377326	22.465821924363855
39	24.49179706651613	26.586528788887996	24.682830974058064	24.238843170537805
40	23.823764383700784	25.07798597912691	28.14312307232528	22.955126564847024
41	21.150266334082755	26.924451754171784	27.07309879265789	24.85218311908757
42	22.075940861527762	27.644442534539436	26.49237915347367	23.787237450459127
43	22.859023617494643	25.905799578475285	26.65626202507662	24.578914778953454
44	23.151629745815516	26.178286593834176	27.28190783883614	23.388175821514167
45	23.547175413271972	26.665442591185485	26.218329488564336	23.56905250697821
46	24.097618717416317	25.964398936616984	26.775609384491876	23.162372961474826
47	22.53047654951353	26.845147289486686	27.945057241806342	22.679318919193438
48	22.174192452012008	27.228582422927193	27.164513791358942	23.432711333701857
49	22.988918861375403	27.251631503796265	26.400573492385014	23.358876142443318
50	22.208375410928	25.78371758234675	28.388654382938995	23.61925262378626
51	22.128484952661484	26.55761977220476	25.76301247580335	25.550882799330406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8188.0
1	6876.0
2	5564.0
3	4476.5
4	3389.0
5	2610.0
6	1831.0
7	2445.5
8	3060.0
9	3890.5
10	4721.0
11	3410.0
12	2099.0
13	1998.0
14	1897.0
15	1978.5
16	2060.0
17	1923.5
18	1787.0
19	1730.5
20	1674.0
21	1605.5
22	1537.0
23	1658.5
24	1780.0
25	2311.0
26	3288.0
27	3734.0
28	4417.5
29	5101.0
30	6060.5
31	7020.0
32	7484.0
33	7948.0
34	8969.0
35	9990.0
36	11247.0
37	12504.0
38	13428.5
39	14353.0
40	16486.5
41	18620.0
42	21162.0
43	23704.0
44	27591.0
45	31478.0
46	33058.5
47	34639.0
48	37359.5
49	40080.0
50	40343.0
51	40606.0
52	38029.0
53	35452.0
54	32302.0
55	29152.0
56	28476.5
57	27801.0
58	25858.0
59	23915.0
60	23078.0
61	22241.0
62	20218.5
63	18196.0
64	17125.5
65	16055.0
66	13917.0
67	11779.0
68	10394.0
69	9009.0
70	8288.0
71	7567.0
72	7114.5
73	6662.0
74	5756.0
75	3737.5
76	2625.0
77	2317.5
78	2010.0
79	1614.0
80	1218.0
81	930.5
82	643.0
83	494.0
84	345.0
85	241.5
86	138.0
87	92.0
88	46.0
89	28.0
90	10.0
91	7.0
92	4.0
93	6.0
94	8.0
95	9.5
96	11.0
97	9.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	511951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.742578914478408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8578247619689	19.527760402156584
2	8.507389749024778	4.380043039409901
3	3.1343698350227016	2.4206028847569767
4	1.6740693103925774	1.7237944532434952
5	1.0864841279401474	1.398445170141375
6	0.8145150907409825	1.258063140025997
7	0.6202376696334733	1.1176562009390312
8	0.5350163221913734	1.1018159915636347
9	0.4544364228618798	1.0528528929421697
>10	5.960737292727499	36.842767761092894
>50	1.221935178537892	20.740067658837003
>100	0.12234549984116955	5.113322032543627
>500	0.006839189432115067	1.1868290589327128
>1k	0.0037995496845083707	2.135979313414586
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3968	0.7750741770208477	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	2678	0.5230969370115499	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2112	0.4125394813175479	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1105	0.2158409691552512	No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA	1056	0.20626974065877396	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	816	0.15939025414541627	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	812	0.15860892937019364	TruSeq Adapter, Index 13 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	788	0.15392098071885785	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	699	0.1365365044701544	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	627	0.12247265851614705	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	626	0.12227732732234138	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	619	0.1209100089657018	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	569	0.11114344927541893	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08340641975501562	0.0
2	0.0	0.0	0.0	0.45062906410965115	0.0
3	0.0	0.0	0.0	0.5766176841143	0.0
4	0.0	0.0	0.0	0.7824967623854626	0.0
5	1.9533119380565718E-4	0.0	0.0	1.4966276069389453	0.0
6	1.9533119380565718E-4	0.0	0.0	2.0900437737205317	0.0
7	1.9533119380565718E-4	0.0	0.0	2.5887243115063745	0.0
8	1.9533119380565718E-4	0.0	0.0	3.2747274641518427	0.0
9	1.9533119380565718E-4	0.0	0.0	3.5790534641010567	0.0
10	1.9533119380565718E-4	0.0	0.0	4.2531414139243795	0.0
11	1.9533119380565718E-4	0.0	0.0	5.093846872063928	0.0
12	1.9533119380565718E-4	0.0	0.0	5.743518422661544	0.0
13	1.9533119380565718E-4	0.0	0.0	6.004090235198291	0.0
14	1.9533119380565718E-4	0.0	0.0	6.1123037165666245	0.0
15	1.9533119380565718E-4	0.0	0.0	6.301384312170501	0.0
16	1.9533119380565718E-4	0.0	0.0	6.684233452029589	0.0
17	1.9533119380565718E-4	0.0	0.0	7.145801062992357	0.0
18	1.9533119380565718E-4	0.0	0.0	7.734138618734996	0.0
19	1.9533119380565718E-4	0.0	0.0	8.020103486466478	0.0
20	1.9533119380565718E-4	0.0	0.0	8.305482360616544	0.0
21	3.9066238761131436E-4	0.0	0.0	8.649069930520694	0.0
22	3.9066238761131436E-4	0.0	0.0	8.975273024176142	0.0
23	3.9066238761131436E-4	0.0	0.0	9.349136929120169	0.0
24	5.859935814169716E-4	0.0	0.0	9.642329051022461	0.0
25	5.859935814169716E-4	0.0	0.0	9.906221493853904	0.0
26	5.859935814169716E-4	0.0	0.0	10.14296290074636	0.0
27	5.859935814169716E-4	0.0	0.0	10.398651433437966	0.0
28	5.859935814169716E-4	0.0	0.0	10.680514346099528	0.0
29	5.859935814169716E-4	0.0	0.0	10.974487792777044	0.0
30	5.859935814169716E-4	0.0	0.0	11.268461239454558	0.0
31	5.859935814169716E-4	0.0	0.0	11.545636203464785	0.0
32	5.859935814169716E-4	0.0	0.0	11.839609650142298	0.0
33	5.859935814169716E-4	0.0	0.0	12.137880383083537	0.0
34	5.859935814169716E-4	0.0	0.0	12.421305945295545	0.0
35	5.859935814169716E-4	0.0	0.0	12.74496973343152	0.0
36	5.859935814169716E-4	0.0	0.0	13.028981289224946	0.0
37	5.859935814169716E-4	0.0	0.0	13.329010002910435	0.0
38	5.859935814169716E-4	0.0	0.0	13.607161622889691	0.0
39	5.859935814169716E-4	0.0	0.0	13.901916394342427	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTA	25	3.8877646E-5	45.0	36
AATTTCG	20	7.0296176E-4	45.0	21
GTCGATC	20	7.0296176E-4	45.0	11
CGACGGT	45	3.8380676E-10	45.0	28
GTTTACG	20	7.0296176E-4	45.0	1
ATGTGCG	20	7.0296176E-4	45.0	1
TTCACGA	25	3.8877646E-5	45.0	19
GTTAGGT	20	7.0296176E-4	45.0	34
TCGATTA	35	1.2101555E-7	45.0	41
GAATACG	20	7.0296176E-4	45.0	1
TGTGCGA	20	7.0296176E-4	45.0	20
ATCCGAT	20	7.0296176E-4	45.0	32
CTAACGG	25	3.8877646E-5	45.0	2
GTGTCGA	20	7.0296176E-4	45.0	9
AACGCTA	20	7.0296176E-4	45.0	16
ACTAATG	20	7.0296176E-4	45.0	1
TTAACGG	45	3.8380676E-10	45.0	2
ACGCTAG	20	7.0296176E-4	45.0	1
GTATCCG	20	7.0296176E-4	45.0	30
CGTACCA	20	7.0296176E-4	45.0	45
>>END_MODULE