FastQCFastQC Report
Sat 18 Jun 2016
SRR3553870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275928
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19870.7201153924212114No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT16910.6128410309935926No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10830.3924936940071323No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA7550.27362210431706824No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.2138238960888348No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.17722014438549186No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC3450.12503261720448813No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC3390.12285813690527964RNA PCR Primer, Index 40 (95% over 23bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT2890.1047374677452089No Hit
CTATATATATATATATATATATATATATATATATATATATATATATATATA2870.10401264097880607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATGC207.0239574E-445.00000429
ATTAGGT207.0239574E-445.00000441
AGATACG207.0239574E-445.00000442
TAGGTCA207.0239574E-445.00000443
CGCATCG207.0239574E-445.00000421
GCGTTGC207.0239574E-445.00000438
GCGTTAG207.0239574E-445.0000041
TCCTACG207.0239574E-445.00000424
CGTTAGG207.0239574E-445.0000042
GCTTCGA207.0239574E-445.00000424
GTCGTTT207.0239574E-445.0000049
GCGATCT207.0239574E-445.0000049
GAGTTAG207.0239574E-445.0000041
TCGAGGT207.0239574E-445.00000436
TAGGCAC207.0239574E-445.00000410
CGGTACC207.0239574E-445.00000414
CCCGGAT207.0239574E-445.00000441
ACGGTAC207.0239574E-445.00000413
CGGGTAC253.8830753E-545.06
CAAACGC253.8830753E-545.034