##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275928
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15787089385637	33.0	31.0	34.0	30.0	34.0
2	32.30802600678438	34.0	31.0	34.0	30.0	34.0
3	32.342067495868484	34.0	31.0	34.0	30.0	34.0
4	35.85233829114841	37.0	35.0	37.0	35.0	37.0
5	35.769780522455136	37.0	35.0	37.0	35.0	37.0
6	35.73395595952567	37.0	35.0	37.0	33.0	37.0
7	35.80217665477951	37.0	35.0	37.0	35.0	37.0
8	35.65873706184222	37.0	35.0	37.0	35.0	37.0
9	37.492237105331824	39.0	37.0	39.0	35.0	39.0
10	37.04636716824679	39.0	37.0	39.0	33.0	39.0
11	36.971467194340555	39.0	37.0	39.0	33.0	39.0
12	36.8598076309762	39.0	35.0	39.0	33.0	39.0
13	36.77919964628454	39.0	35.0	39.0	33.0	39.0
14	37.946446174364326	40.0	37.0	41.0	33.0	41.0
15	38.069637731582155	40.0	37.0	41.0	33.0	41.0
16	38.14911498651822	40.0	37.0	41.0	33.0	41.0
17	38.06086732770868	40.0	37.0	41.0	33.0	41.0
18	38.00561378330579	40.0	37.0	41.0	33.0	41.0
19	37.92805369516685	40.0	36.0	41.0	33.0	41.0
20	37.842085616537645	40.0	36.0	41.0	33.0	41.0
21	37.725171059116875	40.0	36.0	41.0	33.0	41.0
22	37.67974978980024	40.0	35.0	41.0	33.0	41.0
23	37.588352758690675	39.0	35.0	41.0	33.0	41.0
24	37.56990229335189	39.0	35.0	41.0	33.0	41.0
25	37.45926473572816	39.0	35.0	41.0	33.0	41.0
26	37.38586877736221	39.0	35.0	41.0	33.0	41.0
27	37.31020773535125	39.0	35.0	41.0	32.0	41.0
28	37.26211185526659	39.0	35.0	41.0	32.0	41.0
29	37.17770215418515	39.0	35.0	41.0	32.0	41.0
30	37.05685178742281	39.0	35.0	41.0	32.0	41.0
31	36.95852541242643	39.0	35.0	41.0	31.0	41.0
32	36.853726334406076	39.0	35.0	41.0	31.0	41.0
33	36.7036654489577	39.0	35.0	41.0	31.0	41.0
34	36.61828085587545	39.0	35.0	41.0	31.0	41.0
35	36.437541677539066	39.0	35.0	41.0	30.0	41.0
36	36.33860644805891	39.0	35.0	41.0	30.0	41.0
37	36.22998753297962	39.0	35.0	41.0	30.0	41.0
38	36.08850497231162	38.0	35.0	40.0	30.0	41.0
39	36.01576860630309	38.0	35.0	40.0	29.0	41.0
40	35.846057667217536	38.0	35.0	40.0	29.0	41.0
41	35.741298454669334	38.0	35.0	40.0	29.0	41.0
42	35.65975906758285	38.0	35.0	40.0	28.0	41.0
43	35.52489055115827	38.0	34.0	40.0	28.0	41.0
44	35.40561305847903	38.0	34.0	40.0	27.0	41.0
45	35.32110912991795	38.0	34.0	40.0	27.0	41.0
46	35.16358615290945	37.0	34.0	40.0	27.0	41.0
47	35.05560146125076	37.0	34.0	40.0	26.0	41.0
48	34.8958858832739	37.0	34.0	40.0	26.0	41.0
49	34.83395668435244	37.0	34.0	40.0	26.0	41.0
50	34.73813096170015	37.0	34.0	40.0	26.0	41.0
51	33.365461279754136	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	1.0
13	2.0
14	13.0
15	16.0
16	26.0
17	73.0
18	144.0
19	213.0
20	344.0
21	531.0
22	794.0
23	1175.0
24	1578.0
25	1991.0
26	2578.0
27	3102.0
28	3582.0
29	4342.0
30	5369.0
31	6637.0
32	8685.0
33	11665.0
34	19290.0
35	26103.0
36	23597.0
37	32622.0
38	51527.0
39	69899.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.388855063639795	19.612000231944567	23.14516830477516	13.853976399640485
2	29.143109796758576	26.566713055579715	26.560914441448496	17.729262706213213
3	31.48828679945493	26.96645501725088	26.17349453480618	15.371763648488013
4	25.920892406714795	28.197935693369285	26.605128874199067	19.276043025716856
5	22.9501899046128	33.99582499782552	24.544808790699022	18.50917630686266
6	23.44343451914992	37.75260212809138	24.801759879388825	14.002203473369864
7	79.4297787828709	9.074468701980226	7.73317677075179	3.762575744397089
8	80.34958394943608	7.440346757125046	8.132556319039749	4.077512974399119
9	75.08734162535154	10.330593488156332	9.2980777594155	5.283987127076629
10	38.87463396248297	28.076889623380012	17.23348119799368	15.814995216143341
11	30.709460438955087	28.779971586790758	21.691890638137483	18.818677336116668
12	27.233916094053523	24.378098634426372	28.597677655040442	19.79030761647966
13	23.013612246673045	27.78188512945406	29.00503029775884	20.19947232611406
14	21.07796236699429	29.91323823606158	28.12182888289699	20.886970514047142
15	19.14738627468035	28.525557391783362	31.37630106404569	20.950755269490593
16	21.346873097329738	24.3596155518831	32.04894030326752	22.244571047519642
17	21.274390420689453	25.570801078542228	27.219781972108663	25.935026528659648
18	21.951016207126496	26.368835348351745	29.539952451364126	22.140195993157636
19	25.161636368907832	28.674146878895947	24.66984140790351	21.494375344292713
20	25.72990055376765	25.92487895393001	25.433808819692093	22.911411672610246
21	24.33642109535821	28.0997216665217	26.514525528398714	21.04933170972138
22	22.549360702792033	25.410251949784	25.77556464005103	26.264822707372936
23	22.280812385839784	28.76330057116349	25.35335304862138	23.602533994375342
24	22.352570235713664	27.033501493143135	28.834333594270966	21.77959467687223
25	22.9501899046128	27.771012727958016	26.657316401380072	22.621480966049116
26	20.63291873242295	28.637905540575805	26.37572120263257	24.353454524368676
27	22.048505407207678	28.25882114174712	26.81605346322229	22.87661998782291
28	21.02468759966368	28.52193325795135	28.889782841900786	21.563596300484182
29	22.546823809109622	26.98457568641095	26.89107303354498	23.57752747093445
30	22.3869995071178	27.022991505030298	27.080252819576124	23.509756168275782
31	23.94501464150068	28.163506421965153	24.210301238004117	23.68117769853005
32	23.35826737409759	27.20564785016381	25.756719144124556	23.679365631614044
33	23.48076309761967	28.370082050389954	24.442970630019424	23.70618422197095
34	22.263416543446116	28.029413470180625	25.384883012959904	24.322286973413355
35	21.53460322982807	27.149111362384392	26.080354295323417	25.235931112464122
36	23.67392943086602	28.274767330607986	25.224696297584874	22.826606940941115
37	22.588501348177786	29.31888028761126	25.264199356353835	22.82841900785712
38	21.52699254878084	28.594415934591634	26.911368183004264	22.967223333623263
39	23.225986489229076	28.152996433852312	25.201501841059986	23.41951523585863
40	22.06735090313415	27.465860659302425	28.22946565770781	22.237322779855614
41	20.408222434838073	28.08884926502566	26.63919573222	24.86373256791627
42	21.61578382766519	27.442303789394334	26.141964570467657	24.79994781247282
43	22.039807486010844	27.55900089878519	25.762517758255775	24.63867385694819
44	22.39533501493143	26.521773796062742	27.067568351164073	24.015322837841758
45	23.269113681830042	27.308573250993014	25.593995535067123	23.828317532109825
46	23.0012901916442	27.312922211591427	26.593894059319823	23.091893537444548
47	22.15396770171929	27.49159200950973	26.461613174451305	23.89282711431968
48	21.543663564408106	26.711678408860283	27.639094256472703	24.105563770258907
49	21.536777710127282	27.80218027891334	27.252036763213596	23.40900524774579
50	21.343248963497725	26.17023281435737	28.709300977066484	23.77721724507843
51	21.49618741120872	28.043547592125485	25.56174074396219	24.898524252703606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4696.0
1	3870.0
2	3044.0
3	2465.5
4	1887.0
5	1394.0
6	901.0
7	1156.0
8	1411.0
9	2035.5
10	2660.0
11	1960.0
12	1260.0
13	1134.0
14	1008.0
15	1031.0
16	1054.0
17	1006.0
18	958.0
19	959.5
20	961.0
21	866.5
22	772.0
23	956.5
24	1141.0
25	1279.5
26	1760.5
27	2103.0
28	2414.0
29	2725.0
30	3586.0
31	4447.0
32	4788.0
33	5129.0
34	5805.0
35	6481.0
36	6922.0
37	7363.0
38	8209.0
39	9055.0
40	9728.0
41	10401.0
42	11841.5
43	13282.0
44	14496.0
45	15710.0
46	16702.5
47	17695.0
48	18681.0
49	19667.0
50	18564.5
51	17462.0
52	18064.0
53	18666.0
54	17471.5
55	16277.0
56	15700.5
57	15124.0
58	14390.0
59	13656.0
60	13417.5
61	13179.0
62	12022.5
63	10866.0
64	9892.0
65	8918.0
66	7390.0
67	5862.0
68	5600.0
69	5338.0
70	4639.0
71	3940.0
72	3636.0
73	3332.0
74	2905.5
75	2005.0
76	1531.0
77	1167.0
78	803.0
79	741.0
80	679.0
81	493.0
82	307.0
83	211.0
84	115.0
85	116.0
86	117.0
87	71.0
88	25.0
89	16.5
90	8.0
91	8.0
92	8.0
93	6.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	275928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.90220637267693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.039615534485	20.93770838769534
2	7.408754383686193	4.1344118755617405
3	3.0159761007923107	2.524571627381056
4	1.7404857773736848	1.942535733959584
5	1.3014677230809195	1.8156910498390886
6	0.9910377971165085	1.6591284682960772
7	0.8169892193791402	1.5957061262358296
8	0.6884010910507857	1.536632744773999
9	0.6182621119625925	1.5525789336348612
>10	7.899727237303546	50.08154301122032
>50	0.41693726457981556	7.100040590298919
>100	0.055851409273931674	2.9065553332753473
>500	0.0025977399662293803	0.48744600040590297
>1k	0.003896609949344071	1.7254501174219363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1987	0.7201153924212114	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	1691	0.6128410309935926	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1083	0.3924936940071323	No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA	755	0.27362210431706824	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	590	0.2138238960888348	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	489	0.17722014438549186	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC	345	0.12503261720448813	No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	339	0.12285813690527964	RNA PCR Primer, Index 40 (95% over 23bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	289	0.1047374677452089	No Hit
CTATATATATATATATATATATATATATATATATATATATATATATATATA	287	0.10401264097880607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.046026499666579686	0.0
2	0.0	0.0	0.0	0.32037343075005076	0.0
3	0.0	0.0	0.0	0.4051781624191818	0.0
4	0.0	0.0	0.0	0.5447073149517265	0.0
5	0.0	0.0	0.0	1.099924618016294	0.0
6	0.0	0.0	0.0	1.5775854570757588	0.0
7	0.0	0.0	0.0	1.9617436432692585	0.0
8	0.0	0.0	0.0	2.5680612333652255	0.0
9	0.0	0.0	0.0	2.851106085645531	0.0
10	0.0	0.0	0.0	3.404511321794091	0.0
11	0.0	0.0	0.0	4.049969557275811	0.0
12	0.0	0.0	0.0	4.555898640224986	0.0
13	0.0	0.0	0.0	4.755226000985765	0.0
14	0.0	0.0	0.0	4.83858107912209	0.0
15	0.0	0.0	0.0	4.9715867907570095	0.0
16	0.0	0.0	0.0	5.251369922588501	0.0
17	0.0	0.0	0.0	5.6101591719579025	0.0
18	0.0	0.0	0.0	6.045780058566002	0.0
19	0.0	0.0	0.0	6.259241541271636	0.0
20	3.6241338320141484E-4	0.0	0.0	6.474877504276478	0.0
21	3.6241338320141484E-4	0.0	0.0	6.713345510423009	0.0
22	3.6241338320141484E-4	0.0	0.0	6.939853874923894	0.0
23	3.6241338320141484E-4	0.0	0.0	7.21565045954017	0.0
24	3.6241338320141484E-4	0.0	0.0	7.437809863442637	0.0
25	3.6241338320141484E-4	0.0	0.0	7.637499637586616	0.0
26	3.6241338320141484E-4	0.0	0.0	7.829941144066568	0.0
27	3.6241338320141484E-4	0.0	0.0	8.02491954422893	0.0
28	3.6241338320141484E-4	0.0	0.0	8.225696558522513	0.0
29	3.6241338320141484E-4	0.0	0.0	8.439158041228147	0.0
30	3.6241338320141484E-4	0.0	0.0	8.694297063001942	0.0
31	7.248267664028297E-4	0.0	0.0	8.923704734568439	0.0
32	7.248267664028297E-4	0.0	0.0	9.13680380389087	0.0
33	7.248267664028297E-4	0.0	0.0	9.361137688092546	0.0
34	7.248267664028297E-4	0.0	0.0	9.560465048853324	0.0
35	7.248267664028297E-4	0.0	0.0	9.820315444608738	0.0
36	7.248267664028297E-4	0.0	0.0	10.039213128062393	0.0
37	7.248267664028297E-4	0.0	0.0	10.25339943753443	0.0
38	7.248267664028297E-4	0.0	0.0	10.495491577512974	0.0
39	7.248267664028297E-4	0.0	0.0	10.685033776927314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCATGC	20	7.0239574E-4	45.000004	29
ATTAGGT	20	7.0239574E-4	45.000004	41
AGATACG	20	7.0239574E-4	45.000004	42
TAGGTCA	20	7.0239574E-4	45.000004	43
CGCATCG	20	7.0239574E-4	45.000004	21
GCGTTGC	20	7.0239574E-4	45.000004	38
GCGTTAG	20	7.0239574E-4	45.000004	1
TCCTACG	20	7.0239574E-4	45.000004	24
CGTTAGG	20	7.0239574E-4	45.000004	2
GCTTCGA	20	7.0239574E-4	45.000004	24
GTCGTTT	20	7.0239574E-4	45.000004	9
GCGATCT	20	7.0239574E-4	45.000004	9
GAGTTAG	20	7.0239574E-4	45.000004	1
TCGAGGT	20	7.0239574E-4	45.000004	36
TAGGCAC	20	7.0239574E-4	45.000004	10
CGGTACC	20	7.0239574E-4	45.000004	14
CCCGGAT	20	7.0239574E-4	45.000004	41
ACGGTAC	20	7.0239574E-4	45.000004	13
CGGGTAC	25	3.8830753E-5	45.0	6
CAAACGC	25	3.8830753E-5	45.0	34
>>END_MODULE