##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255353
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20194005944712	33.0	31.0	34.0	30.0	34.0
2	32.336220839387046	34.0	31.0	34.0	30.0	34.0
3	32.36268616385944	34.0	31.0	34.0	30.0	34.0
4	35.897365607609856	37.0	35.0	37.0	35.0	37.0
5	35.81259276374274	37.0	35.0	37.0	35.0	37.0
6	35.78505441486883	37.0	35.0	37.0	35.0	37.0
7	35.860592983047	37.0	35.0	37.0	35.0	37.0
8	35.71455984460727	37.0	35.0	37.0	35.0	37.0
9	37.56033020955305	39.0	37.0	39.0	35.0	39.0
10	37.149820053024634	39.0	37.0	39.0	34.0	39.0
11	37.02368485978234	39.0	37.0	39.0	33.0	39.0
12	36.88181066993535	39.0	35.0	39.0	33.0	39.0
13	36.798572955868934	39.0	37.0	39.0	33.0	39.0
14	38.0114978089155	40.0	37.0	41.0	33.0	41.0
15	38.11744917819646	40.0	37.0	41.0	33.0	41.0
16	38.17916766202081	40.0	37.0	41.0	33.0	41.0
17	38.11386981942644	40.0	37.0	41.0	33.0	41.0
18	38.02964915234988	40.0	37.0	41.0	33.0	41.0
19	37.92879660704985	40.0	36.0	41.0	34.0	41.0
20	37.83204035198333	40.0	36.0	41.0	33.0	41.0
21	37.72202010550101	40.0	35.0	41.0	33.0	41.0
22	37.693118937314225	40.0	35.0	41.0	33.0	41.0
23	37.59779990836215	40.0	35.0	41.0	33.0	41.0
24	37.53399803409398	39.0	35.0	41.0	33.0	41.0
25	37.44069973722651	39.0	35.0	41.0	33.0	41.0
26	37.36433486193622	39.0	35.0	41.0	33.0	41.0
27	37.29992990096063	39.0	35.0	41.0	33.0	41.0
28	37.256241359999684	39.0	35.0	41.0	32.0	41.0
29	37.144866126499394	39.0	35.0	41.0	32.0	41.0
30	37.05097649136685	39.0	35.0	41.0	32.0	41.0
31	36.9100460930555	39.0	35.0	41.0	31.0	41.0
32	36.79059184736424	39.0	35.0	41.0	31.0	41.0
33	36.65937349473082	39.0	35.0	41.0	31.0	41.0
34	36.53148778357803	39.0	35.0	41.0	31.0	41.0
35	36.36536872486323	39.0	35.0	41.0	30.0	41.0
36	36.23943325514092	39.0	35.0	41.0	30.0	41.0
37	36.14309994399909	38.0	35.0	41.0	30.0	41.0
38	36.029394602765585	38.0	35.0	41.0	29.0	41.0
39	35.967950249262785	38.0	35.0	40.0	29.0	41.0
40	35.793528957952326	38.0	35.0	40.0	29.0	41.0
41	35.63888029512087	38.0	35.0	40.0	28.0	41.0
42	35.4861427122454	38.0	34.0	40.0	27.0	41.0
43	35.389233727428305	38.0	34.0	40.0	27.0	41.0
44	35.25553645345855	38.0	34.0	40.0	27.0	41.0
45	35.15254960779783	37.0	34.0	40.0	26.0	41.0
46	35.0287171092566	37.0	34.0	40.0	26.0	41.0
47	34.874890837389806	37.0	34.0	40.0	26.0	41.0
48	34.70857988744992	37.0	34.0	40.0	25.0	41.0
49	34.60624312226604	37.0	34.0	40.0	24.0	41.0
50	34.53260780174895	36.0	34.0	40.0	24.0	41.0
51	33.111833422752035	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	3.0
11	2.0
12	3.0
13	7.0
14	15.0
15	16.0
16	33.0
17	69.0
18	155.0
19	235.0
20	363.0
21	539.0
22	821.0
23	1174.0
24	1559.0
25	2064.0
26	2560.0
27	2964.0
28	3376.0
29	3987.0
30	4868.0
31	6141.0
32	7784.0
33	10822.0
34	18273.0
35	23986.0
36	20978.0
37	29581.0
38	47511.0
39	65432.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5446107936856	18.701953765963196	22.16696103041672	14.586474409934484
2	30.704945702615593	26.947402223588522	26.468457390357663	15.879194683438222
3	31.373040457719313	26.713999835521808	26.004393917439778	15.908565789319098
4	26.133626783315645	27.46629176081738	26.76021037544106	19.63987108042592
5	23.35237886376898	33.35852721526671	24.815451551381813	18.4736423695825
6	23.40250555113901	37.54606368438985	25.580275148519892	13.471155615951252
7	80.13455882640893	8.75023986403136	7.383896018452887	3.7313052911068207
8	81.73861282224998	7.40974259162806	6.939413282788924	3.912231303333033
9	76.78781921496908	9.57537213191151	8.70442093885719	4.932387714262218
10	40.58695217992348	26.649383402583876	16.529275160268334	16.234389257224315
11	31.03116078526589	27.56497867657713	21.600686109033376	19.803174429123604
12	26.959933895431032	24.21314807345126	29.002596405759867	19.82432162535784
13	23.922569932603103	26.466107701887193	28.457077065865683	21.15424529964402
14	21.059082916589976	29.550073819379445	28.731990616910707	20.65885264711987
15	20.531186240224315	27.29006512553211	30.752722701515157	21.426025932728418
16	21.757723621809806	24.670554095702812	31.80420829205061	21.76751399043677
17	22.019713886267244	25.196492698343075	27.21565832396721	25.568135091422462
18	22.83975516246138	26.342749057187504	27.779191942135007	23.038303838216116
19	25.140883404541945	28.153967253175015	25.322201031513238	21.382948310769798
20	26.162606274451445	26.46571608714211	25.185527485480886	22.186150152925556
21	23.990710898246743	28.045881583533383	25.988337712891568	21.97506980532831
22	22.650996855333595	26.222131715703362	24.93215274541517	26.194718683547872
23	22.712088755565823	29.239131711787213	24.479054485359484	23.56972504728748
24	23.13424945076032	28.162974392311817	27.22975645479004	21.473019702137826
25	23.53173841701488	28.418698820848004	25.926854197914263	22.122708564222858
26	22.217870947276907	28.61293973440688	26.190410921352008	22.978778396964202
27	22.781796180189776	27.279099912669913	27.22074931565323	22.718354591487078
28	21.509048258685038	27.46315884285675	28.529525793705186	22.49826710475303
29	22.518239456752028	27.94288690557777	27.66170751861149	21.877166119058714
30	24.433235560185313	26.92312210939366	26.554221019529827	22.089421310891197
31	25.35744635857029	27.769009958762968	23.88614976131081	22.987393921355928
32	25.330033326414807	27.29672257619844	25.80388716795965	21.56935692942711
33	24.412088363951078	26.878478028454726	24.69483420989767	24.01459939769652
34	22.77944649171931	27.304554871100006	26.36232979444142	23.553668842739267
35	24.64196621931209	26.029065646379717	25.67504591682886	23.65392221747933
36	25.943302017207554	27.388360426546782	24.63687522762607	22.031462328619597
37	23.570116662032557	28.78015923055535	25.87868558426962	21.771038523142476
38	23.45419869748936	28.901559801529647	25.962882754461468	21.681358746519525
39	24.77707330636413	26.3572388027554	24.435193633910703	24.430494256969766
40	24.63726684237115	25.94956785312881	27.036690385466393	22.37647491903365
41	22.693291247802062	26.59690702674337	25.932336804345358	24.77746492110921
42	22.81234213030589	28.143393655057903	25.850489322623975	23.193774892012232
43	23.398981018433307	26.355672343775087	26.585550199136097	23.65979643865551
44	23.29911925843832	25.851664166859212	27.17767169369461	23.67154488100786
45	23.65431383222441	27.136160530716303	26.109738283865862	23.09978735319342
46	24.908655860710468	26.791931169792406	26.33569999177609	21.963712977721038
47	24.28872971925139	26.748461933088702	26.87808641370965	22.084721933950256
48	23.60105422689375	26.956409362725324	26.700293319444064	22.74224309093686
49	23.029688313824394	27.334317591725966	26.51036016808105	23.125633926368597
50	22.516281383026634	26.45005149733898	27.528950120029922	23.50471699960447
51	23.171061236797687	26.818952587202816	25.859888076505854	24.15009809949364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3298.0
1	3006.5
2	2715.0
3	2257.5
4	1800.0
5	1369.5
6	939.0
7	1043.0
8	1147.0
9	1363.0
10	1579.0
11	1354.5
12	1130.0
13	1054.5
14	979.0
15	970.0
16	961.0
17	924.0
18	887.0
19	835.0
20	783.0
21	826.0
22	869.0
23	860.5
24	852.0
25	1261.0
26	1850.5
27	2031.0
28	2396.5
29	2762.0
30	3160.5
31	3559.0
32	4417.5
33	5276.0
34	5551.0
35	5826.0
36	5719.5
37	5613.0
38	6495.0
39	7377.0
40	8117.5
41	8858.0
42	10407.5
43	11957.0
44	13627.5
45	15298.0
46	15293.5
47	15289.0
48	17522.5
49	19756.0
50	19844.0
51	19932.0
52	18346.0
53	16760.0
54	15668.5
55	14577.0
56	13608.0
57	12639.0
58	12724.0
59	12809.0
60	12121.5
61	11434.0
62	10745.5
63	10057.0
64	9142.0
65	8227.0
66	7033.0
67	5839.0
68	5342.0
69	4845.0
70	4652.0
71	4459.0
72	4032.0
73	3605.0
74	3118.0
75	2338.5
76	2046.0
77	1513.5
78	981.0
79	799.5
80	618.0
81	466.0
82	314.0
83	263.0
84	212.0
85	144.5
86	77.0
87	59.5
88	42.0
89	29.0
90	16.0
91	10.5
92	5.0
93	4.0
94	3.0
95	8.0
96	13.0
97	7.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	255353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.88372958218623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03844893787102	22.12544986743841
2	7.646542347560577	4.570144075064714
3	3.1424864694859065	2.817276476093878
4	1.9381724305127837	2.3167928318837063
5	1.4401970933965849	2.1519230242057072
6	1.1152026628575922	1.999584888370217
7	0.9658100617227325	2.020340469859371
8	0.77972454101089	1.8640861865730969
9	0.6906131648953597	1.8574287359067643
>10	8.002987852022697	50.169373377246394
>50	0.1900169049522337	3.5711348603697624
>100	0.04586614947122882	2.9676565382039763
>500	0.0013104614134636806	0.23692692077242092
>1k	0.002620922826927361	1.331881748011576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2291	0.8971893809745726	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1110	0.4346923670370037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	605	0.23692692077242092	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	482	0.18875830712778	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	427	0.16721949614846898	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	418	0.16369496344276355	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	394	0.15429620956088239	No Hit
GTCTTAGGGGTTTTCTGCATCTTTATTCTGCTATGGCTGAGTTGAGGTTAG	392	0.1535129800707256	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	370	0.14489745567900122	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	355	0.1390232345028255	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	292	0.11435150556288745	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	281	0.11004374336702526	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	279	0.10926051387686848	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	261	0.10221144846545763	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.916147450783817E-4	0.0	0.0	0.10495275168100629	0.0
2	3.916147450783817E-4	0.0	0.0	0.4683712351137445	0.0
3	3.916147450783817E-4	0.0	0.0	0.5936879535388266	0.0
4	3.916147450783817E-4	0.0	0.0	0.7464177041193955	0.0
5	3.916147450783817E-4	0.0	0.0	1.3581199359318277	0.0
6	3.916147450783817E-4	0.0	0.0	1.8605616538673915	0.0
7	3.916147450783817E-4	0.0	0.0	2.3214922088246466	0.0
8	3.916147450783817E-4	0.0	0.0	2.9437680387541953	0.0
9	3.916147450783817E-4	0.0	0.0	3.2402204007785302	0.0
10	3.916147450783817E-4	0.0	0.0	3.9149726065485817	0.0
11	3.916147450783817E-4	0.0	0.0	4.766343062348984	0.0
12	3.916147450783817E-4	0.0	0.0	5.351415491496086	0.0
13	3.916147450783817E-4	0.0	0.0	5.57071974873998	0.0
14	3.916147450783817E-4	0.0	0.0	5.665882131794026	0.0
15	3.916147450783817E-4	0.0	0.0	5.80294729257146	0.0
16	3.916147450783817E-4	0.0	0.0	6.12720430149636	0.0
17	3.916147450783817E-4	0.0	0.0	6.496105391360196	0.0
18	3.916147450783817E-4	0.0	0.0	6.935888750083218	0.0
19	7.832294901567634E-4	0.0	0.0	7.156759466307426	0.0
20	7.832294901567634E-4	0.0	0.0	7.378413412021789	0.0
21	7.832294901567634E-4	0.0	0.0	7.6639005611839295	0.0
22	7.832294901567634E-4	0.0	0.0	7.9329398910527775	0.0
23	7.832294901567634E-4	0.0	0.0	8.19649661449053	0.0
24	7.832294901567634E-4	0.0	0.0	8.422458322400756	0.0
25	7.832294901567634E-4	0.0	0.0	8.606908867332672	0.0
26	7.832294901567634E-4	0.0	0.0	8.781569043637631	0.0
27	7.832294901567634E-4	0.0	0.0	8.975026727706352	0.0
28	7.832294901567634E-4	0.0	0.0	9.166917952794758	0.0
29	7.832294901567634E-4	0.0	0.0	9.388571898509122	0.0
30	7.832294901567634E-4	0.0	0.0	9.605134852537468	0.0
31	7.832294901567634E-4	0.0	0.0	9.801333839821737	0.0
32	7.832294901567634E-4	0.0	0.0	10.008889654713279	0.0
33	7.832294901567634E-4	0.0	0.0	10.223102920271154	0.0
34	7.832294901567634E-4	0.0	0.0	10.42987550567254	0.0
35	7.832294901567634E-4	0.0	0.0	10.673068262366215	0.0
36	7.832294901567634E-4	0.0	0.0	10.893155749100265	0.0
37	7.832294901567634E-4	0.0	0.0	11.112068391599081	0.0
38	7.832294901567634E-4	0.0	0.0	11.333330722568366	0.0
39	7.832294901567634E-4	0.0	0.0	11.55028529134179	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGTT	20	7.02297E-4	45.000004	44
TGGACCG	20	7.02297E-4	45.000004	38
GGCCGTA	20	7.02297E-4	45.000004	42
GGCCGAT	20	7.02297E-4	45.000004	8
CCTACAA	20	7.02297E-4	45.000004	16
ATTCGTA	20	7.02297E-4	45.000004	34
TGACGTG	20	7.02297E-4	45.000004	27
GCGTTTA	20	7.02297E-4	45.000004	32
CTGACGT	20	7.02297E-4	45.000004	26
CCATGGT	20	7.02297E-4	45.000004	43
GCGATAG	20	7.02297E-4	45.000004	9
AACTCCT	20	7.02297E-4	45.000004	30
GCCGTAC	20	7.02297E-4	45.000004	43
TGCGTTG	20	7.02297E-4	45.000004	1
CTTCGGA	25	3.8822574E-5	45.0	13
AATCAGG	25	3.8822574E-5	45.0	2
ACGCATT	50	2.1827873E-11	45.0	17
AACCCGA	25	3.8822574E-5	45.0	24
TATGGCC	50	2.1827873E-11	45.0	10
CCGGATT	25	3.8822574E-5	45.0	42
>>END_MODULE