FastQCFastQC Report
Sat 18 Jun 2016
SRR3553863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870450
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATACATATATATGTATATATACATATATATGTATATATACATA502395.771612384398874No Hit
ATATATATACATATATATGTATATATACATATATATGTATATATACATATA231652.6612671606640244No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATATATATG75470.8670228042966281No Hit
ATATATACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC62170.7142282727324947No Hit
ATATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC58700.6743638347980929TruSeq Adapter, Index 16 (95% over 21bp)
ATATATATATACATATATATGCTGTCTCTTATACACATCTGACGCTCTCTC57190.6570164857257741No Hit
ATGTATACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC53400.6134757883853179No Hit
ATATATATATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG53310.6124418404273652No Hit
ATATATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT50320.5780917916020449No Hit
ATGTATATATACATATATATGTATATATACATATATATGTATATATACATA44660.5130679533574588No Hit
ATATATATATACATATATATGTATATATACATATATATGCTGTCTCTTATA44540.5116893560801884No Hit
ATATATATATACATATATATCTGTCTCTTATACACATCTGACGCTCTCTCT41550.4773393072548681No Hit
ATATATGTATACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC40510.46539146418519156No Hit
ATATATATATACATATATATGTATATATACCTGTCTCTTATACACATCTGA35640.4094433913493021No Hit
ATATATATATACATATATACTGTCTCTTATACACATCTGACGCTCTCTCTC35540.40829456028491007No Hit
ATATATATATACATATATATGTATATATATATGTATATATATATATACATA33690.38704118559365847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31300.3595841231546901No Hit
ATATATATATACATATATATGTATATATACTGTCTCTTATACACATCTGAC30570.35119765638462863No Hit
ATATATGTATATATACATATATATGTATATATACATATATATGTATATATA29830.342696306508128No Hit
ATACATATATATACATATATATGTATATATACATATATATGTATATATACA29690.3410879430179792No Hit
ATATATATATATACATATATATGTATATATACATATATATGTATATATACA26930.3093802056407605No Hit
ATATATATATACATATATATGTATATATACATATATATCTGTCTCTTATAC26260.30168303750933423No Hit
ATATATATACATATATATGTATATATACATATATATGTATATATATATGTA24740.28422080533057614No Hit
ATATATATACATATATATGTATATATACATATATATGCTGTCTCTTATACA22840.2623930151071285No Hit
ATATATATATACATATATATGTATATATATATGTATATATATACATATATA22700.2607846516169797No Hit
ATATATATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC22510.25860187259463496No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATATATATA21050.24182893905451203No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATATACCTG20230.2324085243264978No Hit
ACATATATATATACATATATATGTATATATACATATATATGTATATATACA19960.22930668045263947No Hit
ATATATATACATATATATGCTGTCTCTTATACACATCTGACGCTCTCTCTC19570.2248262393015107No Hit
ATATATATATACATATATATGTCTGTCTCTTATACACATCTGACGCTCTCT18380.21115514963524615No Hit
ATATATATACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG18140.20839795508070538No Hit
ATATATATATACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC17590.20207938422654947No Hit
ATATATATATACATATATATGTATATATATATGTATATATATATACATATA17400.19989660520420474No Hit
ATATATATACATATATATCTGTCTCTTATACACATCTGACGCTCTCTCTCT17170.19725429375610318No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATATACTGT17080.1962203457981504No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16780.19277385260497443No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC16140.18542133379286577TruSeq Adapter, Index 16 (95% over 24bp)
ATATATATATACATATACTGTCTCTTATACACATCTGACGCTCTCTCTCTC15060.17301395829743238No Hit
ATATATATACATATATATGTATATATACCTGTCTCTTATACACATCTGACG14350.1648572577402493No Hit
ATATATATATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC14310.1643977253144925No Hit
ATATATATATACATATATATGTATATATACATATATACTGTCTCTTATACA14040.16129588144063414No Hit
ATATATATATACATATATATGTATCTGTCTCTTATACACATCTGACGCTCT13860.15922798552472858No Hit
ATATATATATACATATATATGTATATATCTGTCTCTTATACACATCTGACG13700.15738985582170142No Hit
ATATATATATACATATATATGTATATATACACTGTCTCTTATACACATCTG13510.15520707679935666No Hit
ATATATATACACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC13450.1545177781607215No Hit
ATATATATATACATATATATGTATATATACATATATATGTCTGTCTCTTAT13390.15382847952208628No Hit
ATATATATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGT13320.1530242977770119No Hit
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC12440.14291458441036245TruSeq Adapter, Index 27 (95% over 23bp)
ATATATATATACATATATATGTATATATACATATATATGTATATATATGTA12100.13900855879142973No Hit
ATATATATACATATATACTGTCTCTTATACACATCTGACGCTCTCTCTCTC11840.1360215980240106No Hit
ATATATATATACATATATATGTATATATATATGTATATATACATATATATA11370.13062209202136826No Hit
ATATATATACATATATATGTATATATACATATATATCTGTCTCTTATACAC11360.13050720891492906No Hit
ATATATACATATATATGTATATATACATATATATGTATATATACATATATA11300.12981791027629389No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT10710.12303980699638119No Hit
GTATATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT10610.12189097593198919No Hit
ATATATGTATATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG10580.12154632661267162No Hit
ATATATATGTATATATACATATATATGTATATATACATATATATGTATATA10410.11959331380320523No Hit
ATATATATATACATATATATGTATATATACATATATATGTATCTGTCTCTT10400.11947843069676604No Hit
ATATATATACATATATATGTATATATACTGTCTCTTATACACATCTGACGC10230.11752541788729966No Hit
ATATATATATACATATATATGTATATATACATATACTGTCTCTTATACACA10070.11568728818427251No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.11247056120397496No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC9480.10890918490435982TruSeq Adapter, Index 16 (95% over 24bp)
ATATATATACATATATATGTATATATACATATATATGTATATATACCTGTC9480.10890918490435982No Hit
ATATGTATATATACATATATATGTATATATACATATATATGTATATATACA9150.10511804239186628No Hit
ATATATATATACACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTAT8950.10282038026308232No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC8810.10121201677293354TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGACG351.2114651E-745.0000041
TTTCGTC207.0323434E-445.036
GAGTACG207.0323434E-445.01
TTCGTAC207.0323434E-445.033
GCACCGT253.8900238E-545.024
CCGTAGT253.8900238E-545.027
TATAGAG453.8380676E-1045.01
CGGTCTA502.1827873E-1145.031
ACCCGTA253.8900238E-545.033
ATCGGCA207.0323434E-445.032
ATTGCGA253.8900238E-545.030
GCTCGAC207.0323434E-445.020
TATCCGT253.8900238E-545.028
GTACGAG302.164892E-644.9999961
TCACGTC302.164892E-644.99999639
ACTCAAG800.042.187530
ACGCATT650.041.53846417
CTATACA1200.041.2499967
GTGATAG603.6379788E-1241.2499961
GTAGTAG1200.041.2499961
Produced by FastQC (version 0.11.5)