FastQCFastQC Report
Sat 18 Jun 2016
SRR3553862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74100
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9101.2280701754385965No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.680161943319838No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.2456140350877193No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.24426450742240216No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC1470.19838056680161945No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT1220.16464237516869096No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT1140.15384615384615385No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT1080.14574898785425103No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.1282051282051282No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC940.12685560053981107No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC930.12550607287449392TruSeq Adapter, Index 15 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC890.12010796221322538No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.11066126855600539No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC760.10256410256410256TruSeq Adapter, Index 15 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACTG206.9905986E-445.018
CGGCCTA206.9905986E-445.042
CTATAGG206.9905986E-445.02
TAGCGTA206.9905986E-445.015
GGCCGGT253.8554663E-545.09
ATAGCGT206.9905986E-445.014
TAACGGG206.9905986E-445.03
GCTTGTC253.8554663E-545.038
TCTAGCG253.8554663E-545.01
ACGGGCT253.8554663E-545.05
ACCTCAG206.9905986E-445.014
TATTGCG206.9905986E-445.01
ATCAAAT206.9905986E-445.041
TCTACGA206.9905986E-445.022
GGGGTAT206.9905986E-445.06
TACGAGA206.9905986E-445.024
CTACGAG206.9905986E-445.023
CTTGCCA206.9905986E-445.037
CCTCAGA206.9905986E-445.015
TCAGACG206.9905986E-445.022
Produced by FastQC (version 0.11.5)