##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74100
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19819163292848	33.0	31.0	34.0	30.0	34.0
2	32.34174089068826	34.0	31.0	34.0	30.0	34.0
3	32.383225371120105	34.0	31.0	34.0	30.0	34.0
4	35.910796221322535	37.0	35.0	37.0	35.0	37.0
5	35.81076923076923	37.0	35.0	37.0	35.0	37.0
6	35.768016194331985	37.0	35.0	37.0	35.0	37.0
7	35.775128205128205	37.0	35.0	37.0	35.0	37.0
8	35.588731443994604	37.0	35.0	37.0	35.0	37.0
9	37.501673414304996	39.0	37.0	39.0	35.0	39.0
10	37.068825910931174	39.0	37.0	39.0	33.0	39.0
11	36.973495276653175	39.0	37.0	39.0	33.0	39.0
12	36.78314439946019	39.0	35.0	39.0	33.0	39.0
13	36.6359649122807	39.0	35.0	39.0	33.0	39.0
14	37.8331983805668	40.0	36.0	41.0	33.0	41.0
15	37.961336032388665	40.0	36.0	41.0	33.0	41.0
16	38.07755735492577	40.0	36.0	41.0	33.0	41.0
17	37.94985155195681	40.0	36.0	41.0	33.0	41.0
18	37.882429149797574	40.0	36.0	41.0	33.0	41.0
19	37.690796221322536	39.0	36.0	41.0	33.0	41.0
20	37.58689608636977	39.0	35.0	41.0	33.0	41.0
21	37.47232118758435	39.0	35.0	41.0	33.0	41.0
22	37.409811066126856	39.0	35.0	41.0	33.0	41.0
23	37.26834008097166	39.0	35.0	41.0	33.0	41.0
24	37.24519568151147	39.0	35.0	41.0	33.0	41.0
25	37.16885290148448	39.0	35.0	41.0	32.0	41.0
26	37.05174089068826	39.0	35.0	41.0	32.0	41.0
27	36.98157894736842	39.0	35.0	41.0	32.0	41.0
28	36.93954116059379	39.0	35.0	41.0	32.0	41.0
29	36.855168690958166	39.0	35.0	41.0	32.0	41.0
30	36.72419703103914	39.0	35.0	41.0	31.0	41.0
31	36.62353576248313	39.0	35.0	41.0	31.0	41.0
32	36.476909581646424	39.0	35.0	41.0	31.0	41.0
33	36.34095816464237	38.0	35.0	41.0	30.0	41.0
34	36.18879892037787	38.0	35.0	41.0	30.0	41.0
35	35.96016194331984	38.0	35.0	41.0	30.0	41.0
36	35.83371120107962	38.0	35.0	40.0	29.0	41.0
37	35.703373819163296	38.0	35.0	40.0	29.0	41.0
38	35.589392712550605	38.0	35.0	40.0	28.0	41.0
39	35.48634278002699	38.0	35.0	40.0	27.0	41.0
40	35.29800269905533	38.0	34.0	40.0	27.0	41.0
41	35.156693657219975	37.0	34.0	40.0	26.0	41.0
42	35.01855600539811	37.0	34.0	40.0	26.0	41.0
43	34.86912280701755	37.0	34.0	40.0	25.0	41.0
44	34.76924426450742	37.0	34.0	40.0	25.0	41.0
45	34.68110661268556	37.0	34.0	40.0	25.0	41.0
46	34.51581646423752	36.0	34.0	40.0	24.0	41.0
47	34.37287449392713	36.0	33.0	40.0	23.0	41.0
48	34.21732793522267	36.0	33.0	40.0	23.0	41.0
49	34.068529014844806	36.0	33.0	40.0	23.0	41.0
50	33.98387314439946	35.0	33.0	40.0	23.0	41.0
51	32.612442645074225	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	4.0
15	5.0
16	13.0
17	34.0
18	45.0
19	95.0
20	148.0
21	219.0
22	318.0
23	416.0
24	565.0
25	694.0
26	840.0
27	959.0
28	1137.0
29	1265.0
30	1468.0
31	1835.0
32	2363.0
33	3241.0
34	5821.0
35	7903.0
36	5897.0
37	8395.0
38	13120.0
39	17293.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82995951417004	19.67341430499325	22.214574898785425	14.282051282051281
2	29.32928475033738	28.304993252361673	24.869095816464238	17.496626180836707
3	31.290148448043187	27.966261808367072	25.221322537112012	15.522267206477732
4	26.195681511470987	29.707152496626183	25.89068825910931	18.206477732793523
5	23.817813765182187	33.97840755735493	23.198380566801617	19.005398110661268
6	23.766531713900136	40.282051282051285	22.72199730094467	13.229419703103915
7	78.89068825910931	10.645074224021592	6.8852901484480435	3.578947368421052
8	81.16869095816465	9.31578947368421	5.851551956815115	3.6639676113360324
9	77.0701754385965	11.024291497975709	7.149797570850202	4.755735492577598
10	42.11875843454791	28.978407557354924	14.578947368421053	14.323886639676115
11	32.896086369770586	29.337381916329285	18.973009446693656	18.793522267206477
12	28.676113360323885	25.16194331983806	26.615384615384613	19.54655870445344
13	23.87719298245614	29.19028340080972	26.388663967611336	20.543859649122805
14	20.615384615384617	33.198380566801625	25.292847503373817	20.893387314439945
15	19.63562753036437	30.20917678812416	30.210526315789473	19.944669365722
16	21.846153846153847	25.105263157894736	30.908232118758434	22.140350877192983
17	20.84075573549258	27.302294197031042	25.781376518218625	26.075573549257758
18	23.64102564102564	27.479082321187587	25.309041835357625	23.57085020242915
19	24.912280701754387	29.670715249662617	23.307692307692307	22.10931174089069
20	26.64642375168691	28.246963562753034	23.198380566801617	21.908232118758434
21	25.931174089068826	29.39136302294197	23.248313090418353	21.42914979757085
22	23.685560053981106	27.56140350877193	22.063427800269906	26.68960863697706
23	21.682860998650472	31.854251012145752	22.641025641025642	23.82186234817814
24	22.186234817813766	30.224021592442647	26.520917678812417	21.068825910931174
25	23.560053981106613	28.97165991902834	25.530364372469638	21.937921727395413
26	21.865047233468285	30.11875843454791	25.201079622132255	22.815114709851553
27	22.56815114709852	29.647773279352226	25.238866396761132	22.545209176788124
28	21.453441295546558	29.717948717948715	27.614035087719298	21.214574898785425
29	22.545209176788124	29.573549257759783	25.844804318488528	22.036437246963565
30	23.569500674763834	29.067476383265856	25.419703103913633	21.94331983805668
31	25.45748987854251	29.197031039136302	22.130904183535762	23.214574898785425
32	25.42914979757085	28.989203778677464	23.900134952766532	21.681511470985154
33	24.913630229419702	29.1497975708502	21.763832658569502	24.172739541160592
34	24.20782726045884	28.012145748987855	24.751686909581647	23.02834008097166
35	23.337381916329285	27.695006747638327	24.55735492577598	24.41025641025641
36	25.73144399460189	29.483130904183536	23.86234817813765	20.923076923076923
37	23.780026990553306	30.02294197031039	24.638326585695005	21.558704453441297
38	22.696356275303646	31.37246963562753	24.36707152496626	21.564102564102562
39	24.65991902834008	28.025641025641022	24.016194331983804	23.29824561403509
40	23.67341430499325	26.950067476383268	27.25371120107962	22.12280701754386
41	21.92037786774629	26.920377867746286	25.69905533063428	25.460188933873145
42	23.481781376518217	28.257759784075574	23.87719298245614	24.383265856950068
43	23.67476383265857	27.472334682861	24.091767881241566	24.761133603238868
44	23.781376518218625	27.836707152496626	25.261808367071524	23.120107962213226
45	24.012145748987855	27.952766531713902	24.0944669365722	23.940620782726047
46	23.49122807017544	29.047233468286098	25.197031039136302	22.26450742240216
47	23.808367071524966	28.379217273954115	25.570850202429153	22.24156545209177
48	23.46288798920378	27.2442645074224	25.70715249662618	23.58569500674764
49	22.974358974358974	27.125506072874494	26.628879892037787	23.271255060728745
50	21.88259109311741	27.838056680161944	26.77327935222672	23.506072874493928
51	22.757085020242915	27.817813765182187	24.04183535762483	25.383265856950064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1213.0
1	1141.0
2	1069.0
3	870.0
4	671.0
5	495.0
6	319.0
7	354.0
8	389.0
9	428.5
10	468.0
11	453.0
12	438.0
13	406.0
14	374.0
15	330.0
16	286.0
17	293.5
18	301.0
19	296.0
20	291.0
21	257.5
22	224.0
23	276.0
24	328.0
25	359.5
26	432.5
27	474.0
28	572.5
29	671.0
30	737.5
31	804.0
32	967.0
33	1130.0
34	1300.0
35	1470.0
36	1625.5
37	1781.0
38	1709.5
39	1638.0
40	1963.0
41	2288.0
42	2801.5
43	3315.0
44	3671.5
45	4028.0
46	4474.0
47	4920.0
48	4998.5
49	5077.0
50	5328.0
51	5579.0
52	5175.0
53	4771.0
54	4603.5
55	4436.0
56	4356.5
57	4277.0
58	4123.0
59	3969.0
60	3839.5
61	3710.0
62	3470.5
63	3231.0
64	2749.5
65	2268.0
66	2064.5
67	1861.0
68	1708.5
69	1556.0
70	1383.0
71	1210.0
72	1084.5
73	959.0
74	823.0
75	562.5
76	438.0
77	363.0
78	288.0
79	273.5
80	259.0
81	190.5
82	122.0
83	87.0
84	52.0
85	46.5
86	41.0
87	31.0
88	21.0
89	11.5
90	2.0
91	1.5
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	74100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.695006747638324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.34345923283439	26.179487179487182
2	7.366408002647935	5.4062078272604595
3	4.0491339046007875	4.4574898785425106
4	2.883306976573131	4.232118758434548
5	2.088926483027472	3.832658569500675
6	1.728513110955831	3.8056680161943324
7	1.4710749880475156	3.7786774628879893
8	1.1547938656172998	3.390013495276653
9	1.0407855540436173	3.437246963562753
>10	6.800044132249641	37.21457489878542
>50	0.044132249641425474	1.205128205128205
>100	0.022066124820712737	1.1524966261808367
>500	0.007355374940237578	1.9082321187584346
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	910	1.2280701754385965	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	504	0.680161943319838	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	182	0.2456140350877193	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.24426450742240216	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	147	0.19838056680161945	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	122	0.16464237516869096	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	114	0.15384615384615385	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	108	0.14574898785425103	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.1282051282051282	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	94	0.12685560053981107	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	93	0.12550607287449392	TruSeq Adapter, Index 15 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	89	0.12010796221322538	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.11066126855600539	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	76	0.10256410256410256	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06612685560053981	0.0
2	0.0	0.0	0.0	0.3508771929824561	0.0
3	0.0	0.0	0.0	0.4318488529014845	0.0
4	0.0	0.0	0.0	0.5587044534412956	0.0
5	0.0	0.0	0.0	1.0148448043184886	0.0
6	0.0	0.0	0.0	1.3940620782726045	0.0
7	0.0	0.0	0.0	1.7044534412955465	0.0
8	0.0	0.0	0.0	2.313090418353576	0.0
9	0.0	0.0	0.0	2.6302294197031038	0.0
10	0.0	0.0	0.0	3.2402159244264506	0.0
11	0.0	0.0	0.0	3.819163292847503	0.0
12	0.0	0.0	0.0	4.238866396761134	0.0
13	0.0	0.0	0.0	4.396761133603239	0.0
14	0.0	0.0	0.0	4.469635627530365	0.0
15	0.0	0.0	0.0	4.566801619433198	0.0
16	0.0	0.0	0.0	4.808367071524966	0.0
17	0.0	0.0	0.0	5.0728744939271255	0.0
18	0.0	0.0	0.0	5.423751686909582	0.0
19	0.0	0.0	0.0	5.574898785425101	0.0
20	0.0	0.0	0.0	5.744939271255061	0.0
21	0.0	0.0	0.0	5.904183535762483	0.0
22	0.0	0.0	0.0	6.089068825910931	0.0
23	0.0	0.0	0.0	6.269905533063428	0.0
24	0.0	0.0	0.0	6.442645074224021	0.0
25	0.0	0.0	0.0	6.564102564102564	0.0
26	0.0	0.0	0.0	6.678812415654521	0.0
27	0.0	0.0	0.0	6.805668016194332	0.0
28	0.0	0.0	0.0	6.933873144399461	0.0
29	0.0	0.0	0.0	7.105263157894737	0.0
30	0.0	0.0	0.0	7.2631578947368425	0.0
31	0.0	0.0	0.0	7.4089068825910935	0.0
32	0.0	0.0	0.0	7.566801619433198	0.0
33	0.0	0.0	0.0	7.709851551956815	0.0
34	0.0	0.0	0.0	7.825910931174089	0.0
35	0.0	0.0	0.0	7.98110661268556	0.0
36	0.0	0.0	0.0	8.152496626180836	0.0
37	0.0	0.0	0.0	8.331983805668017	0.0
38	0.0	0.0	0.0	8.484480431848853	0.0
39	0.0	0.0	0.0	8.662618083670715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACTG	20	6.9905986E-4	45.0	18
CGGCCTA	20	6.9905986E-4	45.0	42
CTATAGG	20	6.9905986E-4	45.0	2
TAGCGTA	20	6.9905986E-4	45.0	15
GGCCGGT	25	3.8554663E-5	45.0	9
ATAGCGT	20	6.9905986E-4	45.0	14
TAACGGG	20	6.9905986E-4	45.0	3
GCTTGTC	25	3.8554663E-5	45.0	38
TCTAGCG	25	3.8554663E-5	45.0	1
ACGGGCT	25	3.8554663E-5	45.0	5
ACCTCAG	20	6.9905986E-4	45.0	14
TATTGCG	20	6.9905986E-4	45.0	1
ATCAAAT	20	6.9905986E-4	45.0	41
TCTACGA	20	6.9905986E-4	45.0	22
GGGGTAT	20	6.9905986E-4	45.0	6
TACGAGA	20	6.9905986E-4	45.0	24
CTACGAG	20	6.9905986E-4	45.0	23
CTTGCCA	20	6.9905986E-4	45.0	37
CCTCAGA	20	6.9905986E-4	45.0	15
TCAGACG	20	6.9905986E-4	45.0	22
>>END_MODULE