FastQCFastQC Report
Sat 18 Jun 2016
SRR3553861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292244
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40111.3724832674066876No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19210.6573274387155938No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC12200.41745938325508825No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.3202803137104611No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7790.266558081603044No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC6950.2378149765264642No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG5520.1888832619318104No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC5320.18203966548500566No Hit
GAATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC4220.1443998850275797No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT4140.1416624464488578No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4070.13926718769247615No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC3830.13105487195631046No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC3770.12900179302226905No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.12044729746376315No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA2990.10231176687973065No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2970.10162740723505016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAATC253.883642E-545.00000410
GATCCAC253.883642E-545.00000432
CGTCTGG253.883642E-545.0000042
CCTCGTG253.883642E-545.00000415
TAGCCGT253.883642E-545.00000444
TCGTGTA253.883642E-545.00000417
CATAATT253.883642E-545.00000437
ATGCGAG253.883642E-545.0000041
CTTAACG351.2077726E-745.024
CGTATTA351.2077726E-745.017
GACACGC207.0246425E-445.013
GACGTGT207.0246425E-445.016
CGGCATC207.0246425E-445.033
CTATACG207.0246425E-445.02
CGCATCG207.0246425E-445.021
GCTATAC207.0246425E-445.01
TCGGGTC207.0246425E-445.05
CCGGAAT207.0246425E-445.043
GAATCAT351.2077726E-745.09
TGAACGA351.2077726E-745.032