##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	292244
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.400210098410916	33.0	31.0	34.0	31.0	34.0
2	32.5673615198259	34.0	31.0	34.0	31.0	34.0
3	32.59945114356496	34.0	31.0	34.0	31.0	34.0
4	36.09953668852055	37.0	35.0	37.0	35.0	37.0
5	36.0122671466309	37.0	35.0	37.0	35.0	37.0
6	35.993385664034165	37.0	35.0	37.0	35.0	37.0
7	35.98164889612789	37.0	35.0	37.0	35.0	37.0
8	35.80670261836	37.0	35.0	37.0	35.0	37.0
9	37.75752453429326	39.0	38.0	39.0	35.0	39.0
10	37.387778705465294	39.0	37.0	39.0	35.0	39.0
11	37.32716497173595	39.0	37.0	39.0	35.0	39.0
12	37.15263273155308	39.0	37.0	39.0	34.0	39.0
13	37.010525451335184	39.0	37.0	39.0	33.0	39.0
14	38.228740367638004	40.0	37.0	41.0	33.0	41.0
15	38.35017656478833	40.0	37.0	41.0	34.0	41.0
16	38.45613254677598	40.0	37.0	41.0	34.0	41.0
17	38.350436621453305	40.0	37.0	41.0	34.0	41.0
18	38.26575738081877	40.0	37.0	41.0	34.0	41.0
19	38.08543887984013	40.0	36.0	41.0	34.0	41.0
20	37.95555426287623	40.0	35.0	41.0	34.0	41.0
21	37.90199285528531	40.0	35.0	41.0	34.0	41.0
22	37.82061564993636	40.0	35.0	41.0	33.0	41.0
23	37.70827459246383	40.0	35.0	41.0	33.0	41.0
24	37.70583142853232	40.0	35.0	41.0	33.0	41.0
25	37.668824680746226	40.0	35.0	41.0	33.0	41.0
26	37.57465337183997	40.0	35.0	41.0	33.0	41.0
27	37.520062002983806	39.0	35.0	41.0	33.0	41.0
28	37.424922325180326	39.0	35.0	41.0	33.0	41.0
29	37.34532787670577	39.0	35.0	41.0	33.0	41.0
30	37.193697732032135	39.0	35.0	41.0	33.0	41.0
31	37.097589685331435	39.0	35.0	41.0	33.0	41.0
32	36.946530980961114	39.0	35.0	41.0	32.0	41.0
33	36.843377451718425	39.0	35.0	41.0	32.0	41.0
34	36.67738259810295	39.0	35.0	41.0	31.0	41.0
35	36.52200900617292	39.0	35.0	41.0	31.0	41.0
36	36.373496119680816	39.0	35.0	41.0	31.0	41.0
37	36.27961908542177	39.0	35.0	41.0	31.0	41.0
38	36.13057582020503	38.0	35.0	41.0	30.0	41.0
39	36.0889872845978	38.0	35.0	41.0	30.0	41.0
40	35.91492383077155	38.0	35.0	41.0	30.0	41.0
41	35.75399323852671	38.0	35.0	40.0	29.0	41.0
42	35.59631335459411	38.0	35.0	40.0	29.0	41.0
43	35.50516691531734	38.0	35.0	40.0	29.0	41.0
44	35.383795732333255	38.0	35.0	40.0	28.0	41.0
45	35.27852410999028	37.0	35.0	40.0	27.0	41.0
46	35.093439044086445	37.0	34.0	40.0	27.0	41.0
47	34.94366693584813	37.0	34.0	40.0	26.0	41.0
48	34.82099204774093	37.0	34.0	40.0	26.0	41.0
49	34.7268686440098	36.0	34.0	40.0	26.0	41.0
50	34.609254595475015	36.0	34.0	40.0	26.0	41.0
51	33.25805149121967	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	1.0
12	3.0
13	4.0
14	9.0
15	10.0
16	14.0
17	48.0
18	126.0
19	191.0
20	409.0
21	599.0
22	1015.0
23	1377.0
24	1828.0
25	2124.0
26	2559.0
27	3058.0
28	3258.0
29	3854.0
30	4571.0
31	5697.0
32	7753.0
33	10973.0
34	20348.0
35	31250.0
36	24113.0
37	33982.0
38	56267.0
39	76774.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.925240552415104	20.73883467239704	22.87335240415543	14.462572371032426
2	29.526696869738984	28.92959307975527	25.10060086776803	16.44310918273771
3	30.556658134983095	28.611365844978852	24.991103324619154	15.840872695418899
4	25.385294479955107	30.032780826980193	25.851001218160167	18.730923474904532
5	22.875063303267133	33.83953135051532	24.185269843007898	19.100135503209646
6	22.135954887012222	38.210194221267166	25.077332639848894	14.576518251871724
7	78.01871039268556	10.115519908022064	8.03780402677215	3.8279656725202225
8	79.50240210235283	8.973323661050356	7.324701277015097	4.19957295958172
9	75.3011182436594	11.236501006008677	8.21026265723163	5.252118093100286
10	39.33699237623356	28.113152023651473	15.933261247450762	16.61659435266421
11	30.803027607068067	29.525328150449624	20.674504865797076	18.997139376685233
12	26.35879607451308	25.21762636700839	28.600758270486303	19.822819287992225
13	23.560107307592286	29.01171623711693	27.774051819712298	19.65412463557849
14	19.774914112864593	32.45472960950438	27.65394670207087	20.116409575560148
15	18.380531336828128	29.827472933576054	30.583005981303295	21.208989748292524
16	20.927033574684167	24.86039063248518	32.119051203788615	22.093524589042033
17	20.168078728733523	26.456659503702383	26.33279040801522	27.04247135954887
18	22.572918520140707	27.31587303759872	26.7119256511682	23.399282791092375
19	23.501936737794445	30.583690340947978	23.874912744145306	22.03946017711228
20	24.059689848209032	27.629309754862376	25.46331147944868	22.847688917479914
21	23.68671384185817	29.924652003120677	26.299598965248215	20.08903518977293
22	22.903122048699032	26.11037352349407	24.730020120173553	26.25648430763335
23	20.040103475178274	31.534950247053832	24.599991787684264	23.824954490083627
24	21.179904463393605	28.370813429873664	29.599581171897455	20.849700934835276
25	21.578543956419978	29.45928744473796	25.899590752932482	23.062577845909583
26	19.633936026060415	30.176838532185435	25.486579707367817	24.702645734386337
27	21.49607861923598	30.02388415159935	25.642271526532625	22.837765702632048
28	18.782592628077907	30.422181464803383	28.76124060716388	22.033985299954832
29	22.059990966452688	27.4968861636167	27.36069859432529	23.082424275605316
30	21.271950835603125	29.69710242126442	27.674819671233625	21.356127071898825
31	23.529311123581664	29.917466226851534	24.074403580569662	22.47881906899714
32	23.88517813881551	28.735577120488358	24.903163110277713	22.476081630418417
33	23.570030522440153	28.786561914017057	22.87232586468841	24.771081698854385
34	21.136789805778733	29.044223320239254	25.960840941131387	23.858145932850633
35	21.616525916699743	28.90666703165848	24.686563282736344	24.790243768905434
36	24.539425959130043	29.59718591314107	24.013837752015437	21.849550375713445
37	22.60953176113111	29.70907871504633	25.037639780457425	22.64374974336513
38	21.869738985231518	30.4410013550321	24.705725352787397	22.98353430694899
39	23.894074814196355	27.15675942021051	24.68485238362464	24.264313381968492
40	24.966808557232998	26.332448228192877	25.981713910294136	22.719029304279985
41	20.965015534963936	27.760364626818685	25.758954845950644	25.515664992266736
42	21.419430339031766	28.144290387484432	26.405332530351348	24.03094674313245
43	21.65553441644653	27.167709174525395	27.154364161454126	24.022392247573947
44	22.982507767481966	25.918410643161195	26.706792953833098	24.39228863552374
45	22.97497981139048	27.298421866659368	25.839709284022938	23.88688903792721
46	24.50452361725134	27.861649854231395	24.297847004557834	23.335979523959434
47	20.87844403991185	28.76671548432132	27.89723655575478	22.457603920012044
48	22.21773586455154	27.425370580747593	26.555891652181053	23.80100190251981
49	22.160591834220718	27.092771793432885	27.016123513228674	23.730512859117724
50	21.76024144208264	26.625696335938464	28.31572247847689	23.298339743502005
51	21.229520537632936	28.26165806654713	24.374495284762048	26.13432611105788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5184.0
1	4796.0
2	4408.0
3	3587.5
4	2767.0
5	1988.5
6	1210.0
7	1312.0
8	1414.0
9	1708.5
10	2003.0
11	1786.0
12	1569.0
13	1498.5
14	1428.0
15	1393.5
16	1359.0
17	1390.5
18	1422.0
19	1303.0
20	1184.0
21	1202.5
22	1221.0
23	1198.0
24	1175.0
25	1415.0
26	1734.5
27	1814.0
28	2080.5
29	2347.0
30	3175.5
31	4004.0
32	4378.5
33	4753.0
34	5403.0
35	6053.0
36	6341.0
37	6629.0
38	7118.5
39	7608.0
40	8045.0
41	8482.0
42	11187.0
43	13892.0
44	15772.5
45	17653.0
46	19671.5
47	21690.0
48	22258.5
49	22827.0
50	23387.0
51	23947.0
52	22103.5
53	20260.0
54	18753.5
55	17247.0
56	16737.5
57	16228.0
58	14831.0
59	13434.0
60	12371.0
61	11308.0
62	10585.0
63	9862.0
64	9054.0
65	8246.0
66	7134.0
67	6022.0
68	5563.5
69	5105.0
70	4967.5
71	4830.0
72	4228.0
73	3626.0
74	3271.0
75	2274.0
76	1632.0
77	1284.5
78	937.0
79	787.0
80	637.0
81	382.5
82	128.0
83	103.0
84	78.0
85	49.5
86	21.0
87	16.5
88	12.0
89	8.5
90	5.0
91	4.5
92	4.0
93	5.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	292244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.157293220733358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29044332101887	21.199750893089337
2	8.171300797200077	4.601634250831497
3	3.16692591872448	2.6751618510559667
4	1.7924849309741395	2.018860951807394
5	1.3076025665953723	1.840927444190471
6	0.9527513124635426	1.6096138842884713
7	0.7704647093136302	1.5185940515459684
8	0.6331421349406962	1.4262054995141045
9	0.5407835893447405	1.3704301884726462
>10	6.655891503013805	43.033560996975126
>50	0.6319268909196967	11.244713321744843
>100	0.07656037332296325	3.8177002778500153
>500	0.006076220104997083	1.1955762992567853
>1k	0.00364573206299825	2.4472700893773696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4011	1.3724832674066876	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1921	0.6573274387155938	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1220	0.41745938325508825	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.3202803137104611	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	779	0.266558081603044	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	695	0.2378149765264642	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	552	0.1888832619318104	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	532	0.18203966548500566	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC	422	0.1443998850275797	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	414	0.1416624464488578	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	407	0.13926718769247615	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	383	0.13105487195631046	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	377	0.12900179302226905	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	352	0.12044729746376315	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	299	0.10231176687973065	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	297	0.10162740723505016	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4217982234023623E-4	0.0	0.0	0.08143879771697622	0.0
2	3.4217982234023623E-4	0.0	0.0	0.491028045058239	0.0
3	3.4217982234023623E-4	0.0	0.0	0.634401390618798	0.0
4	3.4217982234023623E-4	0.0	0.0	0.8612666128303746	0.0
5	3.4217982234023623E-4	0.0	0.0	1.722191045838409	0.0
6	3.4217982234023623E-4	0.0	0.0	2.333666388360411	0.0
7	3.4217982234023623E-4	0.0	0.0	2.8917616785973363	0.0
8	3.4217982234023623E-4	0.0	0.0	3.6113658449788533	0.0
9	3.4217982234023623E-4	0.0	0.0	3.9494395094510066	0.0
10	3.4217982234023623E-4	0.0	0.0	4.855531679007952	0.0
11	3.4217982234023623E-4	0.0	0.0	5.926554522932892	0.0
12	3.4217982234023623E-4	0.0	0.0	6.770027785001574	0.0
13	3.4217982234023623E-4	0.0	0.0	7.045482541985464	0.0
14	3.4217982234023623E-4	0.0	0.0	7.160797142114124	0.0
15	3.4217982234023623E-4	0.0	0.0	7.353102202269336	0.0
16	3.4217982234023623E-4	0.0	0.0	7.770561585524425	0.0
17	3.4217982234023623E-4	0.0	0.0	8.286226577791162	0.0
18	3.4217982234023623E-4	0.0	0.0	8.886409986175936	0.0
19	3.4217982234023623E-4	0.0	0.0	9.214218255977881	0.0
20	3.4217982234023623E-4	0.0	0.0	9.482487236692627	0.0
21	3.4217982234023623E-4	0.0	0.0	9.800372291646706	0.0
22	3.4217982234023623E-4	0.0	0.0	10.103543614240156	0.0
23	3.4217982234023623E-4	0.0	0.0	10.44503907693571	0.0
24	3.4217982234023623E-4	0.0	0.0	10.715703316406838	0.0
25	3.4217982234023623E-4	0.0	0.0	10.93572494217161	0.0
26	3.4217982234023623E-4	0.0	0.0	11.157457467048083	0.0
27	3.4217982234023623E-4	0.0	0.0	11.392192825173485	0.0
28	3.4217982234023623E-4	0.0	0.0	11.634113959568031	0.0
29	3.4217982234023623E-4	0.0	0.0	11.892459725434911	0.0
30	3.4217982234023623E-4	0.0	0.0	12.163808324550718	0.0
31	3.4217982234023623E-4	0.0	0.0	12.39854368267612	0.0
32	3.4217982234023623E-4	0.0	0.0	12.637385198669605	0.0
33	3.4217982234023623E-4	0.0	0.0	12.871778376972667	0.0
34	3.4217982234023623E-4	0.0	0.0	13.126702344616143	0.0
35	3.4217982234023623E-4	0.0	0.0	13.374440535990473	0.0
36	3.4217982234023623E-4	0.0	0.0	13.622520907187145	0.0
37	3.4217982234023623E-4	0.0	0.0	13.878129234475301	0.0
38	3.4217982234023623E-4	0.0	0.0	14.131342303007076	0.0
39	3.4217982234023623E-4	0.0	0.0	14.36607766113248	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAATC	25	3.883642E-5	45.000004	10
GATCCAC	25	3.883642E-5	45.000004	32
CGTCTGG	25	3.883642E-5	45.000004	2
CCTCGTG	25	3.883642E-5	45.000004	15
TAGCCGT	25	3.883642E-5	45.000004	44
TCGTGTA	25	3.883642E-5	45.000004	17
CATAATT	25	3.883642E-5	45.000004	37
ATGCGAG	25	3.883642E-5	45.000004	1
CTTAACG	35	1.2077726E-7	45.0	24
CGTATTA	35	1.2077726E-7	45.0	17
GACACGC	20	7.0246425E-4	45.0	13
GACGTGT	20	7.0246425E-4	45.0	16
CGGCATC	20	7.0246425E-4	45.0	33
CTATACG	20	7.0246425E-4	45.0	2
CGCATCG	20	7.0246425E-4	45.0	21
GCTATAC	20	7.0246425E-4	45.0	1
TCGGGTC	20	7.0246425E-4	45.0	5
CCGGAAT	20	7.0246425E-4	45.0	43
GAATCAT	35	1.2077726E-7	45.0	9
TGAACGA	35	1.2077726E-7	45.0	32
>>END_MODULE