##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553860_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 483103 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10258267905602 33.0 31.0 34.0 30.0 34.0 2 32.237195794685604 34.0 31.0 34.0 30.0 34.0 3 32.28569476902441 34.0 31.0 34.0 30.0 34.0 4 35.823141234891935 37.0 35.0 37.0 35.0 37.0 5 35.74101589102117 37.0 35.0 37.0 35.0 37.0 6 35.719596028176184 37.0 35.0 37.0 33.0 37.0 7 35.82538092290878 37.0 35.0 37.0 35.0 37.0 8 35.724232720558554 37.0 35.0 37.0 35.0 37.0 9 37.55751465008497 39.0 37.0 39.0 35.0 39.0 10 37.08744719035071 39.0 37.0 39.0 33.0 39.0 11 36.98549791659335 39.0 37.0 39.0 33.0 39.0 12 36.85175004088155 39.0 35.0 39.0 33.0 39.0 13 36.759535751175214 39.0 35.0 39.0 33.0 39.0 14 37.91046836802918 40.0 37.0 41.0 33.0 41.0 15 38.009670815540375 40.0 37.0 41.0 33.0 41.0 16 38.10651558777321 40.0 37.0 41.0 33.0 41.0 17 38.003957748140664 40.0 37.0 41.0 33.0 41.0 18 37.941708082955394 40.0 36.0 41.0 33.0 41.0 19 37.83740941372751 40.0 36.0 41.0 33.0 41.0 20 37.747915040891904 40.0 36.0 41.0 33.0 41.0 21 37.64573807241934 39.0 35.0 41.0 33.0 41.0 22 37.620911068654095 39.0 35.0 41.0 33.0 41.0 23 37.53996145749457 39.0 35.0 41.0 33.0 41.0 24 37.49325506155002 39.0 35.0 41.0 33.0 41.0 25 37.38035988184714 39.0 35.0 41.0 33.0 41.0 26 37.296690353816885 39.0 35.0 41.0 33.0 41.0 27 37.26504492830721 39.0 35.0 41.0 32.0 41.0 28 37.18109181685893 39.0 35.0 41.0 32.0 41.0 29 37.06226622480092 39.0 35.0 41.0 32.0 41.0 30 36.9538090220926 39.0 35.0 41.0 32.0 41.0 31 36.84012726064628 39.0 35.0 41.0 31.0 41.0 32 36.70047588195478 39.0 35.0 41.0 31.0 41.0 33 36.56813350362138 39.0 35.0 41.0 31.0 41.0 34 36.459618342258274 39.0 35.0 41.0 30.0 41.0 35 36.299366801696536 39.0 35.0 41.0 30.0 41.0 36 36.20106271333442 39.0 35.0 41.0 30.0 41.0 37 36.11749875285395 38.0 35.0 41.0 30.0 41.0 38 36.001003926698864 38.0 35.0 40.0 29.0 41.0 39 35.94494134791132 38.0 35.0 40.0 29.0 41.0 40 35.77694818703258 38.0 35.0 40.0 29.0 41.0 41 35.622591869642704 38.0 35.0 40.0 28.0 41.0 42 35.489278683841746 38.0 34.0 40.0 28.0 41.0 43 35.4171781172959 38.0 34.0 40.0 27.0 41.0 44 35.29984289064651 38.0 34.0 40.0 27.0 41.0 45 35.2166080525271 38.0 34.0 40.0 27.0 41.0 46 35.08425946433783 37.0 34.0 40.0 26.0 41.0 47 34.94551472460324 37.0 34.0 40.0 26.0 41.0 48 34.775743474993945 37.0 34.0 40.0 25.0 41.0 49 34.71152114559421 37.0 34.0 40.0 26.0 41.0 50 34.61291277429451 37.0 34.0 40.0 25.0 41.0 51 33.27322951834288 35.0 32.0 39.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 4.0 11 2.0 12 8.0 13 19.0 14 31.0 15 44.0 16 70.0 17 156.0 18 253.0 19 430.0 20 722.0 21 1094.0 22 1579.0 23 2263.0 24 2966.0 25 3882.0 26 4780.0 27 5611.0 28 6567.0 29 7849.0 30 9344.0 31 11655.0 32 14771.0 33 21011.0 34 34562.0 35 45238.0 36 40615.0 37 56836.0 38 90348.0 39 120344.0 40 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.431317959110174 20.310161601149236 22.19630182383467 14.062218615905925 2 29.697807713882963 27.081595436169927 25.669060221112268 17.551536628834842 3 31.95488332715798 27.37573560917651 25.058424393969815 15.610956669695696 4 27.60715623790372 28.19005470883021 25.225262521656873 18.9775265316092 5 22.61877901813899 33.43096606727758 24.338495103528647 19.611759811054785 6 22.219692280942162 38.63751622324846 25.236440262221514 13.906351233587868 7 79.88979575784046 8.928323773605214 7.412290960726802 3.7695895078275234 8 81.33689917057025 6.719064050523388 7.132847446610764 4.811189332295598 9 76.34976392197937 9.849866384601214 8.679308553248479 5.121061140170936 10 39.485575539791725 27.34861923854747 16.698716422791826 16.46708879886898 11 30.643361767573374 29.223995711059548 21.302496569054632 18.830145952312446 12 26.976027886392757 23.392941049838235 29.099177608087718 20.531853455681293 13 24.224440750730174 27.27410096811653 28.56471601294134 19.936742268211955 14 20.565800667766503 29.934403222501203 27.709618859746264 21.79017724998603 15 19.749204620960747 28.71395954899887 30.49308325553764 21.043752574502747 16 22.415302740823385 24.885790400804797 30.95778746975283 21.74111938861899 17 21.378049815463783 25.155919131116967 28.37717836569013 25.08885268772912 18 23.722063410908234 25.75330726573836 28.014315787730563 22.51031353562284 19 24.32918031972478 29.17224691214917 24.701564676683855 21.797008091442198 20 26.640074683866587 27.230838972227456 24.93546924775876 21.1936170961472 21 24.10459053245374 29.15071941180245 25.13397764037897 21.610712415364837 22 22.458978727103744 26.462679801201816 24.6943198448364 26.38402162685804 23 21.97295400773748 29.609627760539674 24.361885560636136 24.055532671086706 24 23.27888669703976 26.783522354446156 28.809384334189602 21.128206614324483 25 22.575724017445555 29.441754656874412 25.24389208926461 22.738629236415424 26 21.17457353814818 29.25090508649294 25.886819166927133 23.687702208431745 27 22.60801526796563 28.42209632314434 25.5036710597947 23.466217349095327 28 20.93611507276916 28.40967661140585 27.7661285481564 22.888079767668593 29 22.523354232948254 29.330805232010565 25.76862491021583 22.377215624825347 30 23.69577502106176 26.83879007168244 26.727426656427305 22.738008250828496 31 24.58130046801614 28.350475985452377 23.851021417792893 23.21720212873859 32 24.686868017793305 27.858241410216866 24.830729678764154 22.624160893225667 33 23.70881571838718 27.56906912190568 23.814176272968705 24.90793888673844 34 21.71379602279431 27.711895806898323 25.968996259596818 24.605311910710554 35 22.89718755627682 27.18447204840376 26.032129794267473 23.88621060105195 36 24.293991136465724 28.393737981341456 24.789744630027137 22.522526252165687 37 22.216380357811897 28.788270824234168 26.1948280180417 22.80052079991223 38 23.360028813731233 28.14141083785445 25.8706735416671 22.627886806747217 39 23.857231273662137 25.406797308234474 25.632836061875004 25.103135356228385 40 23.70508980486563 25.11803901031457 28.78951279540802 22.387358389411784 41 21.818535591788915 26.85410771615991 26.98203074706636 24.345325944984815 42 22.768643539783444 26.961952213089134 26.540302999567377 23.729101247560045 43 22.971498831512122 25.79946719436642 26.849139831464512 24.379894142656948 44 22.595802551422782 25.368089206649515 26.53885403319789 25.497254208729814 45 23.419436434880346 26.410517011900154 26.082843617199647 24.087202936019857 46 24.30578986261729 25.869017580101968 26.593086774455966 23.23210578282478 47 22.796380895999405 26.63220886643221 28.062752663510683 22.508657574057704 48 23.53825167717857 25.9011018354264 27.29894039159351 23.261706095801514 49 23.008550971531953 25.294191921805492 27.6758786428567 24.021378463805856 50 21.509905755087424 24.72433414820442 29.34550189090111 24.420258205807045 51 22.250741560288386 25.77069486217225 25.73550567891319 26.24305789862617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5163.0 1 4814.0 2 4465.0 3 3894.5 4 3324.0 5 2464.0 6 1604.0 7 1740.0 8 1876.0 9 2633.5 10 3391.0 11 2665.0 12 1939.0 13 1945.5 14 1952.0 15 1925.5 16 1899.0 17 1862.0 18 1825.0 19 1758.0 20 1691.0 21 1738.0 22 1785.0 23 1794.0 24 1803.0 25 2477.5 26 3666.0 27 4180.0 28 4545.0 29 4910.0 30 5729.5 31 6549.0 32 7347.0 33 8145.0 34 9445.0 35 10745.0 36 10954.0 37 11163.0 38 11956.0 39 12749.0 40 15011.5 41 17274.0 42 19645.0 43 22016.0 44 24544.5 45 27073.0 46 30626.0 47 34179.0 48 36969.5 49 39760.0 50 39945.0 51 40130.0 52 36867.0 53 33604.0 54 32011.0 55 30418.0 56 28853.0 57 27288.0 58 25203.0 59 23118.0 60 21651.0 61 20184.0 62 18683.0 63 17182.0 64 15433.5 65 13685.0 66 11841.5 67 9998.0 68 9059.5 69 8121.0 70 7689.0 71 7257.0 72 6444.5 73 5632.0 74 5011.0 75 3866.5 76 3343.0 77 2477.0 78 1611.0 79 1501.5 80 1392.0 81 902.5 82 413.0 83 426.0 84 439.0 85 281.5 86 124.0 87 94.0 88 64.0 89 46.5 90 29.0 91 34.0 92 39.0 93 23.5 94 8.0 95 12.0 96 16.0 97 10.5 98 5.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 483103.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.848914990901463 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56743385223304 21.044710414314075 2 8.808042994634194 4.905888811875457 3 3.2384457610333683 2.7056160210499036 4 1.7340700710094124 1.9316787998323033 5 1.1602737221776132 1.6156182127550582 6 0.8111018881536505 1.3552984519290407 7 0.6571491250269209 1.2810623083453998 8 0.5490954407001621 1.2233369799960312 9 0.4359661852859904 1.092706670964343 >10 6.000625068545868 39.105290681270304 >50 0.947035246951501 16.973772301150703 >100 0.08332124717876521 4.1653365407996645 >500 0.004463638241719564 0.9068230807799119 >1k 0.002975758827813043 1.6928607249377794 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4034 0.8350186192178479 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1911 0.39556781887092396 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1152 0.23845846537901855 No Hit ATATATACATATATATGTATATATACATATATATGTATATATACATATATA 1074 0.22231284011898084 No Hit CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC 940 0.1945754839030186 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC 850 0.17594591629528278 No Hit GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC 749 0.15503940153549037 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 654 0.13537485794954698 No Hit ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC 630 0.13040697325415077 No Hit CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT 554 0.11467533838539606 Illumina Single End Adapter 1 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.1399039128301834E-4 0.0 0.0 0.11736627592873569 0.0 2 4.1399039128301834E-4 0.0 0.0 0.6174666685986219 0.0 3 4.1399039128301834E-4 0.0 0.0 0.8050043158498291 0.0 4 4.1399039128301834E-4 0.0 0.0 1.076582012531489 0.0 5 4.1399039128301834E-4 0.0 0.0 2.024620008569601 0.0 6 4.1399039128301834E-4 0.0 0.0 2.783257400595732 0.0 7 4.1399039128301834E-4 0.0 0.0 3.3967911604771652 0.0 8 4.1399039128301834E-4 0.0 0.0 4.264515020606372 0.0 9 4.1399039128301834E-4 0.0 0.0 4.666292695346541 0.0 10 4.1399039128301834E-4 0.0 0.0 5.537535473801653 0.0 11 4.1399039128301834E-4 0.0 0.0 6.662347366917614 0.0 12 4.1399039128301834E-4 0.0 0.0 7.476045480984387 0.0 13 4.1399039128301834E-4 0.0 0.0 7.782191375338178 0.0 14 4.1399039128301834E-4 0.0 0.0 7.907009478310008 0.0 15 4.1399039128301834E-4 0.0 0.0 8.127873352059499 0.0 16 4.1399039128301834E-4 0.0 0.0 8.601478359687272 0.0 17 4.1399039128301834E-4 0.0 0.0 9.209009258895101 0.0 18 4.1399039128301834E-4 0.0 0.0 9.913206914467516 0.0 19 4.1399039128301834E-4 0.0 0.0 10.258681895993194 0.0 20 4.1399039128301834E-4 0.0 0.0 10.568139713477251 0.0 21 4.1399039128301834E-4 0.0 0.0 10.973229311347684 0.0 22 4.1399039128301834E-4 0.0 0.0 11.364657226305777 0.0 23 4.1399039128301834E-4 0.0 0.0 11.793344276479344 0.0 24 0.0010349759782075458 0.0 0.0 12.124743584701399 0.0 25 0.0010349759782075458 0.0 0.0 12.427163565533643 0.0 26 0.0010349759782075458 0.0 0.0 12.68714953125938 0.0 27 0.0010349759782075458 0.0 0.0 12.969697973310039 0.0 28 0.0012419711738490549 0.0 0.0 13.284537665880775 0.0 29 0.0012419711738490549 0.0 0.0 13.583231733191473 0.0 30 0.0012419711738490549 0.0 0.0 13.917942964543792 0.0 31 0.0012419711738490549 0.0 0.0 14.203803329724717 0.0 32 0.0012419711738490549 0.0 0.0 14.495666555579245 0.0 33 0.0012419711738490549 0.0 0.0 14.800570478759187 0.0 34 0.0012419711738490549 0.0 0.0 15.07504610817983 0.0 35 0.0012419711738490549 0.0 0.0 15.40437546444547 0.0 36 0.0012419711738490549 0.0 0.0 15.699964603821545 0.0 37 0.0012419711738490549 0.0 0.0 16.01894420030511 0.0 38 0.0012419711738490549 0.0 0.0 16.343305671875356 0.0 39 0.0012419711738490549 0.0 0.0 16.646346638294524 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATT 95 0.0 45.000004 17 TACGCAT 95 0.0 45.000004 16 CTACGCA 95 0.0 45.000004 15 CAGTACG 35 1.2099736E-7 45.000004 1 GCGTACA 35 1.2099736E-7 45.000004 24 CGAACAA 25 3.8874372E-5 45.0 38 ATTAGGT 20 7.029222E-4 45.0 15 CTCGTCG 20 7.029222E-4 45.0 35 GTATTAG 20 7.029222E-4 45.0 1 GCGAACC 20 7.029222E-4 45.0 40 CTATATG 20 7.029222E-4 45.0 23 TTATGCA 20 7.029222E-4 45.0 43 TAGAACG 20 7.029222E-4 45.0 1 ACTCTCG 20 7.029222E-4 45.0 1 TCGATTA 25 3.8874372E-5 45.0 41 CCTCGTG 25 3.8874372E-5 45.0 15 CTGACCG 25 3.8874372E-5 45.0 1 CATTAGT 40 6.8012014E-9 45.0 17 TACGAAC 25 3.8874372E-5 45.0 36 AACGATC 25 3.8874372E-5 45.0 24 >>END_MODULE