FastQCFastQC Report
Sat 18 Jun 2016
SRR3553859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192170
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38682.0128011656345945No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17860.9293854399750221No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.351251496071187No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.34292553468283293No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC5520.2872456678982151No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC5160.2685122547744185No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC4790.24925846906384974No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC4750.2471769787167612No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG4030.2097101524691679No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.1842118957173336No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC3440.17900816984961232No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT3300.17172295363480253No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC2880.14986730499037312No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT2690.13998022584170267No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT2460.1280116563459437No Hit
AAAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC2460.1280116563459437No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.1243690482385388No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2150.11188010615600769No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1940.10095228183379298No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC253.8786464E-545.00000443
ACACGCG302.1560409E-645.00000436
GATTTAC253.8786464E-545.00000425
ATCACGC253.8786464E-545.00000442
ACGGGCA253.8786464E-545.0000045
GCACCGT302.1560409E-645.00000424
CTACGAA253.8786464E-545.00000411
TACGAAT253.8786464E-545.00000412
GTACTAG253.8786464E-545.0000041
CGAATGA253.8786464E-545.00000439
CGAATAT253.8786464E-545.00000414
CCCCATA253.8786464E-545.00000425
ACCACGA302.1560409E-645.00000411
GAGCATG253.8786464E-545.0000041
ACCGTAG302.1560409E-645.00000426
CAGTCAC253.8786464E-545.00000430
CACCGTA302.1560409E-645.00000425
TAGTGCC253.8786464E-545.00000430
TTCGCGT253.8786464E-545.00000413
GTTGGAG253.8786464E-545.0000041
Produced by FastQC (version 0.11.5)