FastQCFastQC Report
Sat 18 Jun 2016
SRR3553858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556734
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39390.7075192102512151No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19590.3518736057075731No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17950.3224160909877967No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC8690.15608890421637622No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC7810.14028243290332545No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC6290.11298034608987417No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5780.10381977748799248No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT5660.10166434958166738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA302.163477E-645.00000410
TCCGCAA207.030148E-445.00000434
CCGGCAC207.030148E-445.00000428
CGCATAC207.030148E-445.00000437
TAGCGCG406.8048394E-945.0000041
TTCCGCA207.030148E-445.00000433
TCGATCC302.163477E-645.00000424
GGCTTCG406.8048394E-945.00000420
CGCCTAA302.163477E-645.00000439
ACGATTA207.030148E-445.00000440
TGCGCAC207.030148E-445.0000041
CTATTAC207.030148E-445.00000421
TATAGCG302.163477E-645.0000041
GCGTGTT207.030148E-445.00000414
TCGCCGG253.888205E-545.024
TAGCCGT253.888205E-545.044
GACGGGT453.8380676E-1045.04
GTACGCG253.888205E-545.01
CTACGCA1150.043.04347615
ACGGCCC950.042.6315811