##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361641
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14490060584945	33.0	31.0	34.0	30.0	34.0
2	32.2778446027967	34.0	31.0	34.0	30.0	34.0
3	32.29358120345868	34.0	31.0	34.0	30.0	34.0
4	35.83431082205834	37.0	35.0	37.0	35.0	37.0
5	35.759432697066984	37.0	35.0	37.0	35.0	37.0
6	35.72460810582871	37.0	35.0	37.0	33.0	37.0
7	35.77596566760959	37.0	35.0	37.0	35.0	37.0
8	35.67724898449014	37.0	35.0	37.0	35.0	37.0
9	37.51697125049427	39.0	37.0	39.0	35.0	39.0
10	37.0201442867374	39.0	37.0	39.0	33.0	39.0
11	36.96541044848344	39.0	37.0	39.0	33.0	39.0
12	36.83384074261491	39.0	35.0	39.0	33.0	39.0
13	36.746027137409754	39.0	35.0	39.0	33.0	39.0
14	37.89562024217387	40.0	37.0	41.0	33.0	41.0
15	38.001122660317826	40.0	37.0	41.0	33.0	41.0
16	38.095589272234065	40.0	37.0	41.0	33.0	41.0
17	38.016264748742536	40.0	37.0	41.0	33.0	41.0
18	37.94781565143333	40.0	36.0	41.0	33.0	41.0
19	37.84607939918317	40.0	36.0	41.0	33.0	41.0
20	37.73257456980818	39.0	35.0	41.0	33.0	41.0
21	37.621279113817295	39.0	35.0	41.0	33.0	41.0
22	37.54441559447076	39.0	35.0	41.0	33.0	41.0
23	37.468284846021334	39.0	35.0	41.0	33.0	41.0
24	37.44589247347508	39.0	35.0	41.0	33.0	41.0
25	37.28948598195448	39.0	35.0	41.0	32.0	41.0
26	37.25300781714463	39.0	35.0	41.0	33.0	41.0
27	37.21498392051786	39.0	35.0	41.0	32.0	41.0
28	37.05673029330192	39.0	35.0	41.0	32.0	41.0
29	36.87751112290918	39.0	35.0	41.0	31.0	41.0
30	36.7615480545624	39.0	35.0	41.0	31.0	41.0
31	36.668851153492	39.0	35.0	41.0	31.0	41.0
32	36.557682895468155	39.0	35.0	41.0	31.0	41.0
33	36.43951321891046	39.0	35.0	41.0	30.0	41.0
34	36.34882106840762	39.0	35.0	41.0	30.0	41.0
35	36.188244695706516	39.0	35.0	41.0	30.0	41.0
36	36.056752414687494	38.0	35.0	41.0	30.0	41.0
37	35.949071040064595	38.0	35.0	40.0	29.0	41.0
38	35.81173594808111	38.0	35.0	40.0	29.0	41.0
39	35.73044262127358	38.0	35.0	40.0	28.0	41.0
40	35.5967050196189	38.0	35.0	40.0	28.0	41.0
41	35.441611985366706	38.0	34.0	40.0	27.0	41.0
42	35.32843068125572	38.0	34.0	40.0	27.0	41.0
43	35.18614592925028	38.0	34.0	40.0	26.0	41.0
44	35.04597929991345	37.0	34.0	40.0	26.0	41.0
45	34.95621071725827	37.0	34.0	40.0	26.0	41.0
46	34.77638320876228	37.0	34.0	40.0	25.0	41.0
47	34.67289383670546	37.0	34.0	40.0	24.0	41.0
48	34.521884963264675	37.0	34.0	40.0	24.0	41.0
49	34.43896294944434	36.0	34.0	40.0	24.0	41.0
50	34.30010701220271	36.0	33.0	40.0	24.0	41.0
51	32.87967072317575	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	1.0
11	0.0
12	0.0
13	6.0
14	10.0
15	15.0
16	48.0
17	93.0
18	190.0
19	340.0
20	585.0
21	900.0
22	1313.0
23	1827.0
24	2472.0
25	3077.0
26	3811.0
27	4642.0
28	5252.0
29	6267.0
30	7222.0
31	9101.0
32	11506.0
33	15696.0
34	25970.0
35	35492.0
36	30451.0
37	42356.0
38	64745.0
39	88213.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33634737211765	19.96233834106199	22.859963333803414	13.841350953016942
2	29.96369327592834	27.30608531665381	25.21450831072804	17.51571309668981
3	31.21133942224471	28.00816279127643	25.245478250530223	15.535019535948635
4	26.086920454262653	29.20354716417663	24.349838652143976	20.35969372941674
5	23.59135164431024	32.533092210230585	24.559438780448016	19.316117365011156
6	22.660870863646547	37.71281464214511	25.273406499816115	14.352907994392227
7	79.75036016380886	9.130048860610385	7.34430001023114	3.7752909653496145
8	82.18537168075522	7.107048149960874	6.728219422023499	3.979360747260405
9	76.88038690303367	10.199341335744565	8.349993501842988	4.570278259378776
10	38.50199507246136	28.501469689554003	16.452504002588203	16.544031235396428
11	30.324271860767997	29.105660033016168	21.646605335125166	18.92346277109067
12	26.82135045528577	24.571329025193492	28.66903918526937	19.93828133425137
13	24.774569255145295	27.635693961691288	27.611913472200335	19.977823310963082
14	20.691514513011523	30.710566556336254	28.26062310412813	20.337295826524095
15	19.75412079935627	28.985651516282722	30.401420192953786	20.858807491407223
16	22.237799364563198	24.86830862651082	30.76891171078501	22.124980298140976
17	20.895584294922312	24.74359931534312	27.084041908964966	27.276774480769607
18	23.182382528529676	26.184254550783788	27.463147154222007	23.170215766464533
19	24.64156442438772	28.3554685447723	24.51381342270373	22.489153608136245
20	24.551419778177806	27.20985728941132	24.834296996192357	23.404425936218516
21	25.158375294836592	27.993230856014666	25.440422960892157	21.407970888256585
22	23.036934418387293	26.23928149739659	25.345024485608658	25.37875959860746
23	21.352943941643783	29.748009766591732	23.961884852657747	24.937161439106738
24	22.295868001692288	27.910275660115975	27.679107180878276	22.11474915731347
25	22.245818366833404	29.16289911818627	24.994400524276838	23.596881990703487
26	19.796427949264604	29.997981423566465	25.09477631131426	25.110814315854675
27	22.426937211212223	28.250668480620284	25.77528543500322	23.547108873164273
28	21.83546666445453	28.29961204620051	27.60583009116776	22.2590911981772
29	22.501873404840712	28.37924903426326	26.94163548933887	22.177242071557153
30	23.585821297916993	28.70719857538277	26.491188775608958	21.215791351091276
31	24.948222131893232	30.305192165711297	23.57669622636814	21.169889476027333
32	24.115351965070335	29.68385774843007	24.297023844088475	21.903766442411122
33	23.8844600031523	28.261452656087116	24.04013925412218	23.813948086638405
34	22.033176548013085	29.638232390685793	25.111090833174337	23.217500228126788
35	23.440373187774615	28.869237724704888	24.573264646431127	23.117124441089366
36	24.367535760602365	29.124463210753206	24.018017868549197	22.489983160095235
37	23.105787230983214	29.243918692847327	24.684701126255042	22.965592949914416
38	23.81947843303165	29.456283994347988	24.04124532340083	22.68299224921953
39	24.218769442624037	28.375101274468328	24.06115457041652	23.34497471249112
40	24.556950124571053	26.44418083126637	26.667883342873182	22.3309857012894
41	21.38944422783921	26.304816102156558	25.95806338330001	26.347676286704218
42	22.182495900630737	27.360559228627285	25.39673322438551	25.060211646356468
43	22.665295140761142	26.875824367259245	25.78745219706836	24.67142829491125
44	22.485835400300296	26.828263388277325	26.4842758426174	24.201625368804976
45	23.237409475142474	27.38046847564297	25.5054045310128	23.876717518201755
46	23.388664448997762	28.218592471539456	24.962877549835333	23.42986552962745
47	22.56574890568271	27.28368741376116	26.28269471658357	23.86786896397256
48	21.659325131829632	28.067890532323492	25.669932336211875	24.602851999634996
49	23.144223138416276	26.3551422543351	27.03039754895048	23.470237058298146
50	22.189685350941957	25.92626389153885	27.836169018446473	24.047881739072725
51	22.15788585918079	27.286452586957783	25.61020459516482	24.945456958696607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4373.0
1	4125.0
2	3877.0
3	3311.0
4	2745.0
5	2023.5
6	1302.0
7	1371.0
8	1440.0
9	1598.0
10	1756.0
11	1655.0
12	1554.0
13	1439.0
14	1324.0
15	1374.0
16	1424.0
17	1396.0
18	1368.0
19	1253.0
20	1138.0
21	1246.0
22	1354.0
23	1651.0
24	1948.0
25	2076.5
26	2744.0
27	3283.0
28	3272.5
29	3262.0
30	4018.5
31	4775.0
32	5447.0
33	6119.0
34	7060.5
35	8002.0
36	8818.0
37	9634.0
38	10236.0
39	10838.0
40	12081.5
41	13325.0
42	15206.0
43	17087.0
44	19026.5
45	20966.0
46	22479.5
47	23993.0
48	25940.5
49	27888.0
50	28813.5
51	29739.0
52	27386.5
53	25034.0
54	22429.0
55	19824.0
56	19305.0
57	18786.0
58	18259.5
59	17733.0
60	16645.5
61	15558.0
62	14251.0
63	12944.0
64	11683.0
65	10422.0
66	9319.5
67	8217.0
68	7419.5
69	6622.0
70	6175.5
71	5729.0
72	5286.0
73	4843.0
74	4157.5
75	2885.5
76	2299.0
77	1945.5
78	1592.0
79	1212.5
80	833.0
81	697.5
82	562.0
83	397.0
84	232.0
85	191.5
86	151.0
87	98.0
88	45.0
89	27.0
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	3.5
96	6.0
97	6.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	361641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.992317187192928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00827558135005	21.276477589247214
2	8.108612757985377	4.539577205392918
3	3.089747483097851	2.594675747256182
4	1.6746640309421956	1.8751090694446808
5	1.0997728257371804	1.5392594885945303
6	0.8398262718017739	1.4105210029447774
7	0.6968558365997001	1.3654626728291845
8	0.5958767264055697	1.334397626400871
9	0.5198924458378846	1.3097694822407628
>10	6.482658882319757	42.842895602340455
>50	0.812831843202641	14.350091923453789
>100	0.06498655572960844	3.2523143489508826
>500	0.002999379495212698	0.5353822691285637
>1k	0.002999379495212698	1.7740659717752036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3541	0.9791478289242647	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1788	0.4944129675562229	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1010	0.2792824928589402	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	748	0.20683495510741312	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	647	0.17890670582151913	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	518	0.14323597158508022	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	490	0.13549348663453534	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	423	0.1169668262171601	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	418	0.11558423961884852	No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	401	0.11088344518458913	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7651731966231707E-4	0.0	0.0	0.07521271094815024	0.0
2	5.530346393246341E-4	0.0	0.0	0.41726463537043645	0.0
3	5.530346393246341E-4	0.0	0.0	0.5643718494307891	0.0
4	5.530346393246341E-4	0.0	0.0	0.7490854189652169	0.0
5	5.530346393246341E-4	0.0	0.0	1.427658921416543	0.0
6	5.530346393246341E-4	0.0	0.0	2.0694556203527807	0.0
7	5.530346393246341E-4	0.0	0.0	2.5436828235736546	0.0
8	5.530346393246341E-4	0.0	0.0	3.31046535099726	0.0
9	5.530346393246341E-4	0.0	0.0	3.684316767180712	0.0
10	5.530346393246341E-4	0.0	0.0	4.382522999328063	0.0
11	5.530346393246341E-4	0.0	0.0	5.182764122430808	0.0
12	5.530346393246341E-4	0.0	0.0	5.850276932095642	0.0
13	5.530346393246341E-4	0.0	0.0	6.197029650952188	0.0
14	5.530346393246341E-4	0.0	0.0	6.367640837183837	0.0
15	5.530346393246341E-4	0.0	0.0	6.545717991046369	0.0
16	5.530346393246341E-4	0.0	0.0	6.907679162484342	0.0
17	5.530346393246341E-4	0.0	0.0	7.348724287345738	0.0
18	5.530346393246341E-4	0.0	0.0	7.918349965850111	0.0
19	5.530346393246341E-4	0.0	0.0	8.165832966947885	0.0
20	5.530346393246341E-4	0.0	0.0	8.41469855464397	0.0
21	5.530346393246341E-4	0.0	0.0	8.678496077601821	0.0
22	5.530346393246341E-4	0.0	0.0	8.972710505722526	0.0
23	5.530346393246341E-4	0.0	0.0	9.28711069817858	0.0
24	5.530346393246341E-4	0.0	0.0	9.5423361842269	0.0
25	5.530346393246341E-4	0.0	0.0	9.759678797481481	0.0
26	5.530346393246341E-4	0.0	0.0	9.94107415917996	0.0
27	5.530346393246341E-4	0.0	0.0	10.149291700885685	0.0
28	5.530346393246341E-4	0.0	0.0	10.379907145484058	0.0
29	5.530346393246341E-4	0.0	0.0	10.608586968844794	0.0
30	5.530346393246341E-4	0.0	0.0	10.865195041491424	0.0
31	5.530346393246341E-4	0.0	0.0	11.083920241344316	0.0
32	5.530346393246341E-4	0.0	0.0	11.331956277081416	0.0
33	5.530346393246341E-4	0.0	0.0	11.585246141892098	0.0
34	5.530346393246341E-4	0.0	0.0	11.833005660309533	0.0
35	5.530346393246341E-4	0.0	0.0	12.100674425742657	0.0
36	5.530346393246341E-4	0.0	0.0	12.374703089528012	0.0
37	5.530346393246341E-4	0.0	0.0	12.62246260794545	0.0
38	5.530346393246341E-4	0.0	0.0	12.87907068059208	0.0
39	5.530346393246341E-4	0.0	0.0	13.132637062722424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	60	0.0	45.000004	38
ATTACGG	35	1.2088458E-7	45.000004	2
CGTCTGA	30	2.1613596E-6	45.000004	28
TCGATTA	30	2.1613596E-6	45.000004	41
TCAATGG	60	0.0	45.000004	2
CCGTACT	55	1.8189894E-12	45.000004	44
AATTACG	30	2.1613596E-6	45.000004	1
GCTACTA	30	2.1613596E-6	45.000004	28
GCATCGT	60	0.0	45.000004	35
CGCCAGT	60	0.0	45.000004	26
CATATGG	45	3.8380676E-10	45.0	2
ACGCCAA	20	7.026867E-4	45.0	39
CGAGTAC	20	7.026867E-4	45.0	27
CGAAACT	25	3.8854858E-5	45.0	27
ACGCATG	20	7.026867E-4	45.0	1
GACAATT	20	7.026867E-4	45.0	41
TAGGTCG	25	3.8854858E-5	45.0	28
ACCGAAC	40	6.7921064E-9	45.0	10
GCACGGC	20	7.026867E-4	45.0	37
TTCGTAC	20	7.026867E-4	45.0	33
>>END_MODULE