FastQCFastQC Report
Sat 18 Jun 2016
SRR3553854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005570
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275372.7384468510397086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81370.8091927961255805No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGC21060.20943345565201826No Hit
CGCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGCCGTC21040.20923456348140856No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGCC18880.1877542090555605No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA15770.15682647652575155No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.13405332299094044No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATG13150.13077160217588035No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGCCGT11510.11446244418588462No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC10690.10630786519088677No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGT10460.10402060522887516No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTAT10450.10392115914357031No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT200.001255693740.038
TACGCAT1800.040.016
AATTGCG401.9279469E-840.01
TCATACG200.001255693740.01
CGTTTTT105200.039.1634981
TCACGAC601.4551915E-1136.66666825
CTACGCA2000.036.015
GTTTTTT117200.035.2730372
CGCATTT2050.035.12195218
AGTACGG800.035.02
GCACTTA9300.034.8387136
TCACCGC2100.034.28571324
ACGCATT2100.034.28571317
CTATACG351.4008934E-534.2857131
CAATACG351.4008934E-534.2857131
CGACGGT653.45608E-1133.84615328
CGTTCGA653.45608E-1133.84615314
CCGCTAC2150.033.4883727
AGGGCGA16200.033.456796
ACTTACT9700.033.4020638
Produced by FastQC (version 0.11.5)