##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1005570
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.734132879859185	31.0	31.0	34.0	30.0	34.0
2	31.9214157144704	33.0	31.0	34.0	30.0	34.0
3	31.617706375488527	31.0	31.0	34.0	30.0	34.0
4	35.54877134361606	37.0	35.0	37.0	33.0	37.0
5	35.42309237546864	37.0	35.0	37.0	33.0	37.0
6	35.446151933729126	37.0	35.0	37.0	33.0	37.0
7	35.78284853366747	37.0	35.0	37.0	35.0	37.0
8	35.604518830116255	37.0	35.0	37.0	35.0	37.0
9	37.39224320534622	39.0	37.0	39.0	35.0	39.0
10	36.87946836122796	39.0	37.0	39.0	32.0	39.0
11	36.85978599202442	39.0	37.0	39.0	32.0	39.0
12	36.89960519904134	39.0	37.0	39.0	33.0	39.0
13	35.22880058076514	39.0	35.0	39.0	30.0	39.0
14	37.06409101305727	40.0	36.0	41.0	31.0	41.0
15	37.68713664886582	40.0	36.0	41.0	32.0	41.0
16	37.85154986723948	40.0	37.0	41.0	33.0	41.0
17	37.86902652227095	40.0	37.0	41.0	33.0	41.0
18	37.760077369054365	39.0	37.0	41.0	33.0	41.0
19	37.59117018208578	39.0	37.0	41.0	33.0	41.0
20	37.406039360760566	39.0	35.0	41.0	33.0	41.0
21	37.26228009984387	39.0	35.0	41.0	32.0	41.0
22	37.25506826973756	39.0	35.0	41.0	32.0	41.0
23	37.15232256332229	39.0	35.0	41.0	32.0	41.0
24	37.066464791113496	39.0	35.0	41.0	32.0	41.0
25	36.85951152082898	39.0	35.0	40.0	31.0	41.0
26	36.83643406227314	39.0	35.0	40.0	32.0	41.0
27	36.8099505752956	39.0	35.0	40.0	32.0	41.0
28	36.650066131646724	38.0	35.0	40.0	31.0	41.0
29	36.52175383116044	38.0	35.0	40.0	31.0	41.0
30	36.311761488509006	38.0	35.0	40.0	30.0	41.0
31	36.130979444494166	38.0	35.0	40.0	30.0	41.0
32	35.81900812474517	38.0	35.0	40.0	29.0	41.0
33	35.44564674761578	38.0	35.0	40.0	26.0	41.0
34	35.131631810813765	38.0	34.0	40.0	24.0	41.0
35	34.90225543721471	38.0	34.0	40.0	23.0	41.0
36	34.71456487365375	38.0	34.0	40.0	22.0	41.0
37	34.60907147190151	38.0	34.0	40.0	22.0	41.0
38	34.46168043994948	38.0	34.0	40.0	21.0	41.0
39	34.325260300128285	38.0	33.0	40.0	20.0	41.0
40	34.19588293206838	38.0	33.0	40.0	20.0	41.0
41	34.05321360024663	38.0	33.0	40.0	18.0	41.0
42	33.94567558698052	37.0	33.0	40.0	18.0	41.0
43	33.832299094046164	37.0	33.0	40.0	18.0	41.0
44	33.6896576071283	37.0	33.0	40.0	18.0	41.0
45	33.584454587945146	37.0	33.0	40.0	18.0	41.0
46	32.69593166065018	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	23.0
10	40.0
11	24.0
12	25.0
13	19.0
14	18.0
15	60.0
16	81.0
17	183.0
18	380.0
19	755.0
20	1322.0
21	2177.0
22	3217.0
23	4702.0
24	6800.0
25	10004.0
26	14522.0
27	20702.0
28	25571.0
29	26668.0
30	27422.0
31	30101.0
32	35540.0
33	45287.0
34	64057.0
35	83373.0
36	97999.0
37	129031.0
38	187380.0
39	188083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.489841582386113	21.959684557017415	20.348061298566982	28.202412562029494
2	33.96501486718975	24.133377089610867	20.078761299561442	21.822846743637935
3	24.97220481915729	24.44583668963871	30.051214733931996	20.530743757271992
4	22.256928905993615	25.859363346161878	29.318893761747066	22.564813986097437
5	19.78022415147628	29.773859602016767	27.721590739580538	22.72432550692642
6	19.20532633232893	36.35012977714132	27.925057430114265	16.519486460415486
7	74.97280149566913	5.877760871943276	15.255427270105512	3.894010362282089
8	74.8965263482403	5.521047763954772	15.343039271259087	4.23938661654584
9	68.33308471812008	7.95797408435017	18.0077965730879	5.701144624441859
10	30.397486002963493	26.968485535566895	24.797875831617887	17.836152629851725
11	25.02829241126923	26.547132472130237	29.966486669252262	18.45808844734827
12	20.447507383871834	23.499209403621826	36.11891762880754	19.9343655836988
13	20.064441063277545	25.202521952723334	36.24054019113538	18.49249679286375
14	19.986574778483845	24.85873683582446	35.0215300774685	20.133158308223198
15	19.399146752588084	24.7366170430701	35.91903099734479	19.945205206997027
16	20.698807641437195	24.148095110235985	35.15329614049743	19.99980110782939
17	20.19312429766202	25.080501606054277	33.50746342870213	21.218910667581568
18	21.19166244020804	24.026174209652236	33.78819972751773	20.993963622621994
19	22.308541424266835	26.083912606780235	31.965750768221007	19.641795200731924
20	23.565142158178944	23.967699911492986	31.756317312568992	20.710840617759082
21	23.770001093906938	25.753353819226906	31.492188509999302	18.98445657686685
22	22.577443638931154	23.527949322274928	31.641755422297802	22.252851616496116
23	21.006493829370406	25.868711278180534	31.928160147975778	21.196634744473283
24	20.171842835406785	24.903487574211642	33.73807890052408	21.186590689857493
25	21.927961255805165	25.65012878268047	31.71713555495888	20.704774406555487
26	20.730630388734745	25.288443370426723	32.31580098849409	21.665125252344442
27	21.328003023160992	24.210746143978042	32.401026283600345	22.060224549260617
28	20.80223157015424	25.517766043139712	32.93385840866374	20.746143978042305
29	21.92637011844029	25.356961723201763	31.38816790477043	21.328500253587517
30	22.50643913402349	24.09449367025667	32.304165796513416	21.09490139920642
31	22.460296150442037	25.152699463985602	31.043388327018505	21.343616058553856
32	22.34314866195292	25.264079079527036	31.311992203426914	21.080780055093133
33	21.040007160118144	25.624770030927735	30.14797577493362	23.187247034020505
34	21.57164593215788	25.769762423302208	31.254015135694186	21.40457650884573
35	22.292630050618058	25.21644440466601	29.989657607128294	22.501267937587638
36	22.842268564097974	27.00110385154688	28.434619171216326	21.722008413138816
37	21.990015613035393	26.430283321897036	29.325954433803712	22.25374663126386
38	21.854967829191406	26.657020396392095	29.820102031683522	21.667909742732977
39	22.237835257615085	24.866095846137018	29.30129180464811	23.594777091599788
40	22.700060662112037	24.621756814542994	30.477042871207377	22.201139652137595
41	20.74932625277206	24.881211651103357	30.930815358453412	23.43864673767117
42	21.577513251190865	25.858965561820657	30.08920313851845	22.47431804847002
43	20.78681742693199	24.46522867627316	31.656771781178833	23.091182115616018
44	22.50136738367294	24.8492894577205	29.912984675358256	22.736358483248306
45	22.433445707409728	24.168978788150007	30.322205316387716	23.075370188052545
46	22.893085513688753	24.563779746810265	28.940799745418023	23.60233499408296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8723.0
1	6226.5
2	3730.0
3	22835.5
4	41941.0
5	27378.5
6	12816.0
7	12816.0
8	11905.5
9	10995.0
10	10723.5
11	10452.0
12	10023.5
13	9595.0
14	9067.5
15	8540.0
16	7917.5
17	7295.0
18	6815.5
19	6336.0
20	6336.0
21	6020.0
22	5704.0
23	5961.5
24	6219.0
25	6952.5
26	7686.0
27	8095.0
28	8504.0
29	10499.5
30	12495.0
31	12495.0
32	14268.0
33	16041.0
34	18522.0
35	21003.0
36	23716.0
37	26429.0
38	28825.5
39	31222.0
40	35842.0
41	40462.0
42	44254.5
43	48047.0
44	48047.0
45	56312.0
46	64577.0
47	72003.5
48	79430.0
49	81388.5
50	83347.0
51	79955.5
52	76564.0
53	69196.5
54	61829.0
55	59566.0
56	57303.0
57	57303.0
58	53463.5
59	49624.0
60	46998.0
61	44372.0
62	41441.0
63	38510.0
64	34124.5
65	29739.0
66	26444.5
67	23150.0
68	20261.5
69	17373.0
70	17373.0
71	14727.5
72	12082.0
73	10426.5
74	8771.0
75	7445.0
76	6119.0
77	5083.0
78	4047.0
79	3317.5
80	2588.0
81	2588.0
82	1782.5
83	977.0
84	758.0
85	539.0
86	371.5
87	204.0
88	166.0
89	128.0
90	85.0
91	42.0
92	24.5
93	7.0
94	7.0
95	7.0
96	7.0
97	5.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1005570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.82356719118593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80378960194955	27.913659397539103
2	8.816696282314766	6.4932441591219385
3	3.417382324871665	3.7752062297364883
4	1.8395215350145842	2.709509793769721
5	1.2972409515919947	2.3884519672052895
6	1.0069591475108977	2.224789669628811
7	0.8051312122646667	2.0753462304784254
8	0.6929969600127953	2.0414896096255
9	0.602722675182029	1.99749590344951
>10	5.59579131606171	38.275860470370894
>50	0.08153439961831224	2.00237023071627
>100	0.03357240261454381	2.290212599831115
>500	0.003463819316072287	0.8748961420736834
>1k	0.002664476396978683	1.4372943350047536
>5k	2.6644763969786825E-4	0.7983660320795739
>10k+	2.6644763969786825E-4	2.70180722936896
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27537	2.7384468510397086	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8137	0.8091927961255805	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGC	2106	0.20943345565201826	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGCCGTC	2104	0.20923456348140856	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGCC	1888	0.1877542090555605	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	1577	0.15682647652575155	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1348	0.13405332299094044	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATG	1315	0.13077160217588035	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTATGCCGT	1151	0.11446244418588462	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC	1069	0.10630786519088677	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGT	1046	0.10402060522887516	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTTAGCCTTCGTAT	1045	0.10392115914357031	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.944608530485198E-5	0.0	0.0	0.09636325666040156	0.0
2	9.944608530485198E-5	0.0	0.0	0.3084817566156508	0.0
3	9.944608530485198E-5	0.0	0.0	0.642918941495868	0.0
4	9.944608530485198E-5	0.0	0.0	0.9238541324820748	0.0
5	9.944608530485198E-5	0.0	0.0	1.4517139532802292	0.0
6	9.944608530485198E-5	0.0	0.0	2.454130493153137	0.0
7	9.944608530485198E-5	0.0	0.0	3.2077329275933053	0.0
8	9.944608530485198E-5	0.0	0.0	4.453394592121881	0.0
9	9.944608530485198E-5	0.0	0.0	5.143749316308163	0.0
10	9.944608530485198E-5	0.0	0.0	6.146364748351681	0.0
11	9.944608530485198E-5	0.0	0.0	6.978927374523902	0.0
12	9.944608530485198E-5	0.0	0.0	7.662420318824149	0.0
13	9.944608530485198E-5	0.0	0.0	8.087154549161172	0.0
14	9.944608530485198E-5	0.0	0.0	8.309615441988127	0.0
15	9.944608530485198E-5	0.0	0.0	8.50910428910966	0.0
16	9.944608530485198E-5	0.0	0.0	8.880435971637976	0.0
17	9.944608530485198E-5	0.0	0.0	9.270662410374216	0.0
18	9.944608530485198E-5	0.0	0.0	9.82616824288712	0.0
19	9.944608530485198E-5	0.0	0.0	10.05678371470907	0.0
20	9.944608530485198E-5	0.0	0.0	10.32568592937339	0.0
21	9.944608530485198E-5	0.0	0.0	10.611692870710144	0.0
22	9.944608530485198E-5	0.0	0.0	10.895114213828972	0.0
23	9.944608530485198E-5	0.0	0.0	11.214833378084071	0.0
24	9.944608530485198E-5	0.0	0.0	11.473194307706077	0.0
25	9.944608530485198E-5	0.0	0.0	11.713754388058515	0.0
26	9.944608530485198E-5	0.0	0.0	11.924182304563582	0.0
27	9.944608530485198E-5	0.0	0.0	12.17090804220492	0.0
28	9.944608530485198E-5	0.0	0.0	12.404407450500711	0.0
29	9.944608530485198E-5	0.0	0.0	12.64626032996211	0.0
30	1.9889217060970396E-4	0.0	0.0	12.951957596189226	0.0
31	1.9889217060970396E-4	0.0	0.0	13.193412691309407	0.0
32	1.9889217060970396E-4	0.0	0.0	13.465198842447567	0.0
33	1.9889217060970396E-4	0.0	0.0	13.719681374742683	0.0
34	1.9889217060970396E-4	0.0	0.0	13.963523175910181	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	20	0.0012556937	40.0	38
TACGCAT	180	0.0	40.0	16
AATTGCG	40	1.9279469E-8	40.0	1
TCATACG	20	0.0012556937	40.0	1
CGTTTTT	10520	0.0	39.163498	1
TCACGAC	60	1.4551915E-11	36.666668	25
CTACGCA	200	0.0	36.0	15
GTTTTTT	11720	0.0	35.273037	2
CGCATTT	205	0.0	35.121952	18
AGTACGG	80	0.0	35.0	2
GCACTTA	930	0.0	34.83871	36
TCACCGC	210	0.0	34.285713	24
ACGCATT	210	0.0	34.285713	17
CTATACG	35	1.4008934E-5	34.285713	1
CAATACG	35	1.4008934E-5	34.285713	1
CGACGGT	65	3.45608E-11	33.846153	28
CGTTCGA	65	3.45608E-11	33.846153	14
CCGCTAC	215	0.0	33.48837	27
AGGGCGA	1620	0.0	33.45679	6
ACTTACT	970	0.0	33.40206	38
>>END_MODULE