FastQCFastQC Report
Sat 18 Jun 2016
SRR3553850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866404
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174962.019381258627615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100721.1625061749484074No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA31840.3674959949400049No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC17020.19644415307408553No Hit
CGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTC15130.17462984935434278No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC14320.1652808620458816No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGT11460.13227085747526557No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9590.11068739294832433No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATG9370.10814816182750772No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG200.001255597540.0000041
CGTATGA200.001255597540.00000428
TACGCTG200.001255597540.0000041
CCGTACT200.001255597540.00000411
TCGTAAG200.001255597540.0000041
GCGGATA200.001255597540.0000045
CGACTAT200.001255597540.00000430
GCCGTAC200.001255597540.00000410
CAGACCG200.001255597540.0000041
CCTAGCG257.7930636E-540.01
GTATACG257.7930636E-540.01
ACGCATT3800.039.47368217
CGCTACA3950.038.98734328
CGTTTTT72850.038.7645871
TACGCAT3950.038.4810116
CTACGCA4050.038.0246915
CGCATTT3900.037.9487218
TCTACGC4150.037.5903614
TCACCGC4150.037.10843324
CCGCTAC4100.037.0731727