##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866404
Sequences flagged as poor quality	0
Sequence length	46
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.712875286817695	31.0	31.0	34.0	30.0	34.0
2	31.88385095174999	33.0	31.0	34.0	30.0	34.0
3	31.64062377366679	31.0	31.0	34.0	30.0	34.0
4	35.54620708122308	37.0	35.0	37.0	33.0	37.0
5	35.39377357445257	37.0	35.0	37.0	33.0	37.0
6	35.42760421235359	37.0	35.0	37.0	33.0	37.0
7	35.76477717092719	37.0	35.0	37.0	35.0	37.0
8	35.59069441045978	37.0	35.0	37.0	35.0	37.0
9	37.35760799811635	39.0	37.0	39.0	34.0	39.0
10	36.79138946726931	39.0	37.0	39.0	32.0	39.0
11	36.78691695790878	39.0	37.0	39.0	32.0	39.0
12	36.867973832069104	39.0	37.0	39.0	33.0	39.0
13	35.17351720444504	39.0	35.0	39.0	30.0	39.0
14	37.01103065082802	40.0	36.0	41.0	31.0	41.0
15	37.63183572559684	40.0	36.0	41.0	32.0	41.0
16	37.80591040669249	40.0	37.0	41.0	33.0	41.0
17	37.83653122561761	39.0	37.0	41.0	33.0	41.0
18	37.73550676127996	39.0	37.0	41.0	33.0	41.0
19	37.58311826815204	39.0	36.0	41.0	33.0	41.0
20	37.426357680712464	39.0	35.0	41.0	33.0	41.0
21	37.287443271268366	39.0	35.0	41.0	32.0	41.0
22	37.25910545196005	39.0	35.0	41.0	32.0	41.0
23	37.16114537790684	39.0	35.0	41.0	32.0	41.0
24	37.10001454286915	39.0	35.0	41.0	32.0	41.0
25	36.883220760753645	39.0	35.0	40.0	31.0	41.0
26	36.87561922613469	39.0	35.0	40.0	31.0	41.0
27	36.84120456507588	39.0	35.0	40.0	31.0	41.0
28	36.672620394181	39.0	35.0	40.0	31.0	41.0
29	36.56415944524725	39.0	35.0	40.0	31.0	41.0
30	36.34831672060609	38.0	35.0	40.0	30.0	41.0
31	36.17311554424956	38.0	35.0	40.0	30.0	41.0
32	35.8870157570833	38.0	35.0	40.0	29.0	41.0
33	35.5546465621119	38.0	35.0	40.0	26.0	41.0
34	35.27126952322473	38.0	35.0	40.0	25.0	41.0
35	35.06639512282953	38.0	34.0	40.0	24.0	41.0
36	34.86401609410852	38.0	34.0	40.0	23.0	41.0
37	34.80707268202825	38.0	34.0	40.0	22.0	41.0
38	34.68993333364112	38.0	34.0	40.0	22.0	41.0
39	34.56083189828302	38.0	34.0	40.0	21.0	41.0
40	34.43247145673381	38.0	34.0	40.0	21.0	41.0
41	34.29077889760435	38.0	33.0	40.0	20.0	41.0
42	34.1986371254057	38.0	33.0	40.0	19.0	41.0
43	34.09209675855605	38.0	33.0	40.0	18.0	41.0
44	33.97449688597929	38.0	33.0	40.0	18.0	41.0
45	33.89342039048758	38.0	33.0	40.0	18.0	41.0
46	32.93200169897646	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	16.0
10	30.0
11	15.0
12	19.0
13	17.0
14	22.0
15	20.0
16	78.0
17	173.0
18	377.0
19	646.0
20	1173.0
21	1871.0
22	2856.0
23	4281.0
24	6077.0
25	8673.0
26	12482.0
27	17087.0
28	20878.0
29	21753.0
30	22661.0
31	25239.0
32	30580.0
33	38969.0
34	53867.0
35	70165.0
36	82533.0
37	111667.0
38	165515.0
39	166660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.622434799469993	23.33749613344352	20.92718870180655	25.11288036527994
2	32.04809765421212	25.271582310330977	21.355049145664147	21.32527088979275
3	24.847992391540206	26.170354707503655	28.650606414559487	20.331046486396644
4	22.028522490662557	27.604443192783044	27.583783085027306	22.783251231527093
5	19.98628815194759	31.844959164546793	25.99918744604134	22.16956523746428
6	19.028767180206923	37.81330649442985	26.72206037829927	16.435865947063956
7	76.38018753376024	6.4917751995604815	13.107511045655377	4.0205262210239106
8	76.46213544720477	6.14770938268983	13.188420182732305	4.201734987373095
9	69.16657817830942	9.619877101213753	16.06513820342473	5.148406517052091
10	28.596590043443936	29.600509692937706	24.158360303045693	17.644539960572665
11	24.408012890060526	29.04511059505727	29.45808191097917	17.08879460390303
12	19.76779885596096	26.10595057271204	34.812396988010214	19.31385358331679
13	20.19658265658977	27.639877008878077	34.79877747563492	17.364762858897233
14	19.815582568870873	27.07974570754521	33.92885997756243	19.175811746021488
15	19.280958998342573	26.871182496849045	35.32612961158998	18.521728893218405
16	20.770679729087124	26.922197958458177	33.655546373285446	18.651575939169255
17	20.432269472440108	27.324319832318412	32.189486659803045	20.053924035438435
18	21.43087982049944	25.755652097635746	33.142852526073284	19.670615555791525
19	22.36427809659235	27.525957867230527	31.43441166014931	18.675352376027814
20	23.010512416840182	26.17958827521572	31.334573709262653	19.475325598681447
21	23.48073185257686	28.11402071089238	31.19122257053292	17.21402486599785
22	22.45430538178494	26.096717004999974	31.311836048771706	20.13714156444338
23	19.738020600089566	28.177963167298397	32.02120488825075	20.062811344361293
24	19.41022894631142	26.51915272782674	33.44859903693889	20.62201928892295
25	19.97867045858514	28.955429568653884	30.498704992128385	20.567194980632593
26	20.017451442975794	27.827664692222104	32.16501770536609	19.989866159436016
27	20.867516770467358	26.700130654983123	31.571530140673403	20.860822433876113
28	20.414610274190792	27.838398714687372	31.621160567125727	20.125830443996104
29	21.886671806686024	26.602023998042483	30.583076717097335	20.928227478174154
30	21.39983194906764	26.465136356711188	31.546137829465238	20.588893864755935
31	21.41345146144293	26.891034667429974	31.360773957645627	20.33473991348147
32	21.77448395898449	27.096827807812524	30.74431789326919	20.384370339933795
33	20.575274352380646	26.953361249486385	30.315303253447585	22.156061144685392
34	21.561188544835897	27.2769977977941	30.449767083254464	20.71204657411554
35	20.976126610680467	27.065087418802314	30.335501682817718	21.623284287699505
36	21.45188618704438	29.247787406337	28.331009552125796	20.969316854492824
37	21.017562245788337	28.03230363664064	29.33412126444476	21.616012853126254
38	21.08358225492957	29.212238170645566	28.688810300968136	21.015369273456724
39	20.960083286780762	27.51106873929483	28.80192150544088	22.726926468483523
40	21.697152829395986	27.89887858320137	29.86689812143065	20.537070465971993
41	19.893375376844983	27.964898592342603	29.8289250742148	22.312800956597613
42	21.44553810924234	27.12683690287672	30.11585819086708	21.311766797013863
43	20.56419407112617	26.545583815402512	30.855466964603117	22.034755148868197
44	21.684918352177508	26.868643265728227	28.820850319250603	22.62558806284366
45	20.998402592785816	25.60179777563354	30.769017686898952	22.630781944681697
46	21.22693339365931	27.22125013273254	28.967317787083164	22.58449868652499
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10796.0
1	7519.0
2	4242.0
3	15992.5
4	27743.0
5	18623.0
6	9503.0
7	9503.0
8	8952.5
9	8402.0
10	8445.5
11	8489.0
12	8269.0
13	8049.0
14	7681.5
15	7314.0
16	6878.0
17	6442.0
18	5932.0
19	5422.0
20	5422.0
21	5449.5
22	5477.0
23	5808.0
24	6139.0
25	6704.5
26	7270.0
27	8350.5
28	9431.0
29	11056.5
30	12682.0
31	12682.0
32	14464.5
33	16247.0
34	19306.5
35	22366.0
36	24992.0
37	27618.0
38	30649.0
39	33680.0
40	38646.0
41	43612.0
42	46586.0
43	49560.0
44	49560.0
45	57503.0
46	65446.0
47	69347.0
48	73248.0
49	73913.5
50	74579.0
51	71242.0
52	67905.0
53	61659.0
54	55413.0
55	51149.0
56	46885.0
57	46885.0
58	43434.0
59	39983.0
60	35728.5
61	31474.0
62	27833.0
63	24192.0
64	20841.5
65	17491.0
66	15218.0
67	12945.0
68	11036.0
69	9127.0
70	9127.0
71	7635.5
72	6144.0
73	4976.5
74	3809.0
75	3291.0
76	2773.0
77	2470.5
78	2168.0
79	1681.0
80	1194.0
81	1194.0
82	911.5
83	629.0
84	443.5
85	258.0
86	214.0
87	170.0
88	109.5
89	49.0
90	34.5
91	20.0
92	13.5
93	7.0
94	7.0
95	6.5
96	6.0
97	5.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	866404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.24707952715624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72915551878206	26.69231567094894
2	8.99017701275346	6.337549682634662
3	3.4884695701111363	3.688750930973153
4	1.9623736100852356	2.7667175478666794
5	1.253670810127989	2.209411737272781
6	0.9171019714299341	1.939509967290159
7	0.7582113763176858	1.870731567562484
8	0.6430846635503394	1.8133485023082663
9	0.5259368899837646	1.668396544877068
>10	5.525594625708413	40.57427459038059
>50	0.17848815254333567	3.886388446430575
>100	0.0221886388853608	1.47135438458477
>500	0.003263035130199302	0.8781201224343909
>1k	0.001631517565099651	1.032466842055472
>5k	0.0	0.0
>10k+	6.526070260398604E-4	3.170663462379999
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17496	2.019381258627615	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10072	1.1625061749484074	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	3184	0.3674959949400049	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC	1702	0.19644415307408553	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTC	1513	0.17462984935434278	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC	1432	0.1652808620458816	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGT	1146	0.13227085747526557	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	959	0.11068739294832433	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATG	937	0.10814816182750772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1541959640075531E-4	0.0	0.0	0.10814816182750772	0.0
2	1.1541959640075531E-4	0.0	0.0	0.3281379125673473	0.0
3	1.1541959640075531E-4	0.0	0.0	0.6449647046874206	0.0
4	1.1541959640075531E-4	0.0	0.0	0.9594831048794789	0.0
5	1.1541959640075531E-4	0.0	0.0	1.4960688085465903	0.0
6	1.1541959640075531E-4	0.0	0.0	2.4243886223978652	0.0
7	1.1541959640075531E-4	0.0	0.0	3.1743851598099733	0.0
8	1.1541959640075531E-4	0.0	0.0	4.461198240081994	0.0
9	1.1541959640075531E-4	0.0	0.0	5.155677951625338	0.0
10	1.1541959640075531E-4	0.0	0.0	6.184528233941672	0.0
11	1.1541959640075531E-4	0.0	0.0	7.020858629461545	0.0
12	1.1541959640075531E-4	0.0	0.0	7.757697332883966	0.0
13	1.1541959640075531E-4	0.0	0.0	8.258964640052447	0.0
14	1.1541959640075531E-4	0.0	0.0	8.498460302584014	0.0
15	1.1541959640075531E-4	0.0	0.0	8.72098928444467	1.1541959640075531E-4
16	1.1541959640075531E-4	0.0	0.0	9.144002105253438	1.1541959640075531E-4
17	1.1541959640075531E-4	0.0	0.0	9.594253950812785	1.1541959640075531E-4
18	1.1541959640075531E-4	0.0	0.0	10.180931759317824	1.1541959640075531E-4
19	1.1541959640075531E-4	0.0	0.0	10.43877913767711	1.1541959640075531E-4
20	1.1541959640075531E-4	0.0	0.0	10.73806215114427	1.1541959640075531E-4
21	1.1541959640075531E-4	0.0	0.0	11.050618418197516	1.1541959640075531E-4
22	1.1541959640075531E-4	0.0	0.0	11.361212552111947	1.1541959640075531E-4
23	1.1541959640075531E-4	0.0	0.0	11.697083577638146	1.1541959640075531E-4
24	2.3083919280151061E-4	0.0	0.0	11.951930046491013	1.1541959640075531E-4
25	2.3083919280151061E-4	0.0	0.0	12.207930711307888	1.1541959640075531E-4
26	2.3083919280151061E-4	0.0	0.0	12.449965604960273	1.1541959640075531E-4
27	2.3083919280151061E-4	0.0	0.0	12.715661515874812	1.1541959640075531E-4
28	2.3083919280151061E-4	0.0	0.0	12.967853334010462	1.1541959640075531E-4
29	2.3083919280151061E-4	0.0	0.0	13.230086657032977	1.1541959640075531E-4
30	3.4625878920226593E-4	0.0	0.0	13.576691705024446	1.1541959640075531E-4
31	3.4625878920226593E-4	0.0	0.0	13.857276743874682	1.1541959640075531E-4
32	3.4625878920226593E-4	0.0	0.0	14.159329827655458	1.1541959640075531E-4
33	3.4625878920226593E-4	0.0	0.0	14.449379273410557	1.1541959640075531E-4
34	3.4625878920226593E-4	0.0	0.0	14.715536862710698	1.1541959640075531E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	0.0012555975	40.000004	1
CGTATGA	20	0.0012555975	40.000004	28
TACGCTG	20	0.0012555975	40.000004	1
CCGTACT	20	0.0012555975	40.000004	11
TCGTAAG	20	0.0012555975	40.000004	1
GCGGATA	20	0.0012555975	40.000004	5
CGACTAT	20	0.0012555975	40.000004	30
GCCGTAC	20	0.0012555975	40.000004	10
CAGACCG	20	0.0012555975	40.000004	1
CCTAGCG	25	7.7930636E-5	40.0	1
GTATACG	25	7.7930636E-5	40.0	1
ACGCATT	380	0.0	39.473682	17
CGCTACA	395	0.0	38.987343	28
CGTTTTT	7285	0.0	38.764587	1
TACGCAT	395	0.0	38.48101	16
CTACGCA	405	0.0	38.02469	15
CGCATTT	390	0.0	37.94872	18
TCTACGC	415	0.0	37.59036	14
TCACCGC	415	0.0	37.108433	24
CCGCTAC	410	0.0	37.07317	27
>>END_MODULE