##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553848_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 975750 Sequences flagged as poor quality 0 Sequence length 46 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68681732001025 31.0 31.0 34.0 30.0 34.0 2 31.85362644119908 33.0 31.0 34.0 30.0 34.0 3 31.7405810914681 31.0 31.0 34.0 30.0 34.0 4 35.55214860363823 37.0 35.0 37.0 33.0 37.0 5 35.37979093005381 37.0 35.0 37.0 33.0 37.0 6 35.385181655137075 37.0 35.0 37.0 33.0 37.0 7 35.709327184217265 37.0 35.0 37.0 35.0 37.0 8 35.484383294901356 37.0 35.0 37.0 33.0 37.0 9 37.25054675890341 39.0 37.0 39.0 34.0 39.0 10 36.72998001537279 39.0 37.0 39.0 32.0 39.0 11 36.700883423007944 39.0 37.0 39.0 32.0 39.0 12 36.77028234691263 39.0 37.0 39.0 32.0 39.0 13 35.13765923648476 39.0 35.0 39.0 30.0 39.0 14 36.91273891878043 39.0 36.0 41.0 30.0 41.0 15 37.51134306943377 40.0 36.0 41.0 32.0 41.0 16 37.69751575710992 40.0 36.0 41.0 32.0 41.0 17 37.70759928260313 39.0 37.0 41.0 33.0 41.0 18 37.71246118370484 39.0 37.0 41.0 33.0 41.0 19 37.6320409941071 39.0 36.0 41.0 33.0 41.0 20 37.52367922111196 39.0 36.0 41.0 32.0 41.0 21 37.411698693312836 39.0 35.0 41.0 32.0 41.0 22 37.40015372790162 39.0 35.0 41.0 32.0 41.0 23 37.298709710479116 39.0 35.0 41.0 32.0 41.0 24 37.244516525749425 39.0 35.0 41.0 32.0 41.0 25 37.034690238278245 39.0 35.0 41.0 31.0 41.0 26 37.02103305149885 39.0 35.0 41.0 31.0 41.0 27 36.989016653856005 39.0 35.0 41.0 31.0 41.0 28 36.83413579297976 39.0 35.0 40.0 31.0 41.0 29 36.76540507302075 39.0 35.0 40.0 31.0 41.0 30 36.614811170894185 39.0 35.0 40.0 31.0 41.0 31 36.54130156290033 39.0 35.0 40.0 30.0 41.0 32 36.389240071739685 39.0 35.0 40.0 30.0 41.0 33 36.24781347681271 39.0 35.0 40.0 30.0 41.0 34 36.0906605175506 39.0 35.0 40.0 30.0 41.0 35 35.970014860363825 39.0 35.0 40.0 29.0 41.0 36 35.8129397899052 38.0 35.0 40.0 28.0 41.0 37 35.726444273635664 38.0 35.0 40.0 28.0 41.0 38 35.61405585447092 38.0 35.0 40.0 27.0 41.0 39 35.441871380988985 38.0 34.0 40.0 27.0 41.0 40 35.32112631309249 38.0 34.0 40.0 26.0 41.0 41 35.14785652062516 38.0 34.0 40.0 26.0 41.0 42 35.0901972841404 38.0 34.0 40.0 26.0 41.0 43 35.021125288239816 38.0 34.0 40.0 25.0 41.0 44 34.90604970535485 38.0 34.0 40.0 25.0 41.0 45 34.82437612093262 38.0 34.0 40.0 24.0 41.0 46 33.81880707148348 36.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 16.0 10 26.0 11 38.0 12 23.0 13 21.0 14 24.0 15 42.0 16 74.0 17 144.0 18 351.0 19 595.0 20 1041.0 21 1776.0 22 2712.0 23 3897.0 24 5400.0 25 7413.0 26 9815.0 27 12953.0 28 16220.0 29 18945.0 30 22657.0 31 27840.0 32 34608.0 33 45329.0 34 64391.0 35 83630.0 36 99975.0 37 135426.0 38 193141.0 39 187221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.4927491673072 23.448116833205226 22.05718677940046 22.00194722008711 2 27.585139636177296 27.794312067640277 22.23889315910838 22.38165513707405 3 26.228029720727648 27.49495260056367 25.197130412503206 21.079887266205482 4 23.418396105559825 27.37166282346913 25.33702280297207 23.872918267998973 5 21.181142710735333 31.609121188829103 22.352139379964132 24.85759672047143 6 20.079528567768385 39.098334614399185 23.50048680502178 17.321650012810657 7 78.76197796566744 6.395900589290289 10.286856264411991 4.555265180630284 8 78.24360748142455 6.640840379195491 9.796771714066104 5.318780425313861 9 71.42516013323085 9.339174993594671 12.556802459646427 6.678862413528056 10 33.11975403535742 27.715295926210608 20.125646938252626 19.03930310017935 11 26.40051242633871 28.023879067384062 25.866154240327955 19.70945426594927 12 22.211837048424286 24.06569305662311 32.53692031770433 21.18554957724827 13 21.322264924417116 26.214501665385605 32.74394055854471 19.719292851652575 14 20.60517550602101 27.23750960799385 30.315552139379964 21.841762746605177 15 19.532257238022037 26.59810402254676 32.10863438380733 21.76100435562388 16 20.85493210351012 25.36028695874968 31.287112477581346 22.497668460158852 17 20.311145272867027 26.86856264411991 28.916730719959006 23.90356136305406 18 21.810299769408147 25.949782218806046 29.04217268767615 23.197745324109658 19 23.22777350755829 27.654009736100438 27.29900076863951 21.819215987701767 20 23.868613886753778 25.938098898283375 27.186779400461187 23.006507814501663 21 24.277427619779658 27.981655137074046 27.535639251857546 20.205277991288753 22 22.858006661542404 25.004355623879064 27.52846528311555 24.609172431462977 23 21.697463489623367 27.27050986420702 28.11181142710735 22.92021521906226 24 21.107660773763772 26.7369715603382 29.359467076607736 22.795900589290287 25 22.443966179861643 27.71806302843966 26.42049705354855 23.41747373815014 26 21.057955418908534 27.11094030233154 27.738867537791446 24.092236740968488 27 22.41065846784525 25.262925954394056 28.174019984627208 24.152395593133487 28 21.520471432231616 26.82623622854215 28.744145529080196 22.90914681014604 29 23.330463745836536 26.220138355111455 26.08537022802972 24.36402767102229 30 22.404611837048424 25.722674865488088 27.127440430438128 24.745272867025363 31 23.338970023059186 26.308480655905715 26.02254675890341 24.330002562131696 32 23.031821675634127 26.96202920830131 26.15598257750448 23.85016653856008 33 21.113604919292854 25.780579041762746 25.205739174993596 27.900076863950808 34 21.32205995388163 26.36320778888035 27.315910837817064 24.998821419420956 35 21.999897514732258 25.313041250320268 26.489162182936205 26.197899052011277 36 22.074814245452217 27.560440686651294 26.055034588777865 24.309710479118625 37 20.705508583141174 28.466307968229565 26.780425313861134 24.047758134768127 38 21.124775813476813 27.35618754803997 27.949269792467334 23.569766846015884 39 21.761209326159364 26.79538816295158 26.080655905713552 25.362746605175506 40 21.696848578016912 26.226082500640537 29.798104022546756 22.2789648987958 41 20.488854727132974 27.096182423776582 28.640532923392264 23.774429925698183 42 21.216602613374327 26.97289264668204 29.09392774788624 22.71657699205739 43 21.13830386881886 24.885472713297464 29.514014860363822 24.46220855751986 44 22.0651806302844 25.031411734563157 27.87722264924417 25.026184985908273 45 22.24411990776326 24.06968998206508 28.41106840891622 25.275121701255443 46 23.13225723802203 25.694593902126567 26.900025621316935 24.27312323853446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9178.0 1 6854.5 2 4531.0 3 11485.5 4 18440.0 5 11911.5 6 5383.0 7 5383.0 8 5186.0 9 4989.0 10 4982.0 11 4975.0 12 5032.5 13 5090.0 14 4974.0 15 4858.0 16 4722.5 17 4587.0 18 4169.5 19 3752.0 20 3752.0 21 3697.0 22 3642.0 23 4299.0 24 4956.0 25 6256.5 26 7557.0 27 8707.0 28 9857.0 29 11779.0 30 13701.0 31 13701.0 32 15499.5 33 17298.0 34 20287.0 35 23276.0 36 24578.0 37 25880.0 38 27518.0 39 29156.0 40 34396.5 41 39637.0 42 44669.0 43 49701.0 44 49701.0 45 59546.0 46 69391.0 47 77894.0 48 86397.0 49 88180.5 50 89964.0 51 84591.5 52 79219.0 53 71953.0 54 64687.0 55 59358.0 56 54029.0 57 54029.0 58 52022.5 59 50016.0 60 46602.0 61 43188.0 62 40903.0 63 38618.0 64 34782.5 65 30947.0 66 27630.5 67 24314.0 68 21531.0 69 18748.0 70 18748.0 71 15790.0 72 12832.0 73 10924.5 74 9017.0 75 7505.0 76 5993.0 77 4906.0 78 3819.0 79 2933.5 80 2048.0 81 2048.0 82 1653.0 83 1258.0 84 854.0 85 450.0 86 337.0 87 224.0 88 152.5 89 81.0 90 51.0 91 21.0 92 30.0 93 39.0 94 39.0 95 22.0 96 5.0 97 3.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 975750.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.09837316380512 #Duplication Level Percentage of deduplicated Percentage of total 1 75.22222248572862 23.39288745071961 2 9.438217602993495 5.87026426038172 3 3.581727826506477 3.3415772555964924 4 1.783090912537392 2.2180490633231047 5 1.143685520993933 1.7783379556955101 6 0.7947714455456847 1.4829659396109918 7 0.6576260373156417 1.4315769937473384 8 0.5216512040892413 1.297800304489239 9 0.443557048735111 1.2414512358900518 >10 5.983902524554188 42.49881583986304 >50 0.3710474716151934 7.191774002835784 >100 0.04765442871248071 2.6514069531178044 >500 0.006244373417493329 1.372820067622573 >1k 0.003943814789995787 2.114216491873179 >5k 3.286512324996489E-4 0.8707881906003813 >10k+ 3.286512324996489E-4 1.2452679946332212 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12184 1.248680502177812 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8520 0.8731744811683321 No Hit ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 4647 0.4762490392006149 No Hit GAATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG 3053 0.3128875224186523 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCG 1566 0.16049192928516526 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTA 1560 0.15987701767870868 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC 1463 0.14993594670766078 No Hit GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT 1379 0.14132718421726878 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC 1236 0.1266717909300538 No Hit CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT 1219 0.12492954137842685 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1208 0.12380220343325646 No Hit CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC 1188 0.12175249807840124 No Hit CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT 1143 0.11714066102997693 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGT 1024 0.10494491416858827 No Hit GAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT 983 0.10074301819113503 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0248526774276198E-4 0.0 0.0 0.1393799641301563 0.0 2 1.0248526774276198E-4 0.0 0.0 0.5124263387138099 0.0 3 1.0248526774276198E-4 0.0 0.0 0.8063540866000513 0.0 4 1.0248526774276198E-4 0.0 0.0 1.2160901870356136 0.0 5 1.0248526774276198E-4 0.0 0.0 2.237355880092237 0.0 6 1.0248526774276198E-4 0.0 0.0 3.3862157314885986 0.0 7 1.0248526774276198E-4 0.0 0.0 4.25826287471176 0.0 8 1.0248526774276198E-4 0.0 0.0 5.7387650525237 0.0 9 1.0248526774276198E-4 0.0 0.0 6.424596464258263 0.0 10 1.0248526774276198E-4 0.0 0.0 7.640276710222905 0.0 11 1.0248526774276198E-4 0.0 0.0 8.659082756853703 0.0 12 1.0248526774276198E-4 0.0 0.0 9.656571867794005 0.0 13 1.0248526774276198E-4 0.0 0.0 10.119292851652576 0.0 14 1.0248526774276198E-4 0.0 0.0 10.335946707660774 0.0 15 1.0248526774276198E-4 0.0 0.0 10.615526518063028 0.0 16 1.0248526774276198E-4 0.0 0.0 11.116986933128363 0.0 17 1.0248526774276198E-4 0.0 0.0 11.655342044581092 0.0 18 1.0248526774276198E-4 0.0 0.0 12.355726364335126 0.0 19 1.0248526774276198E-4 0.0 0.0 12.680604663079682 0.0 20 1.0248526774276198E-4 0.0 0.0 13.021368178324366 0.0 21 1.0248526774276198E-4 0.0 0.0 13.38252626184986 0.0 22 1.0248526774276198E-4 0.0 0.0 13.755982577504485 0.0 23 2.0497053548552396E-4 0.0 0.0 14.150345887778633 0.0 24 2.0497053548552396E-4 0.0 0.0 14.455239559313348 0.0 25 2.0497053548552396E-4 0.0 0.0 14.741070971047911 0.0 26 2.0497053548552396E-4 0.0 0.0 14.992364847553164 0.0 27 2.0497053548552396E-4 0.0 0.0 15.274814245452216 0.0 28 2.0497053548552396E-4 0.0 0.0 15.551012042018959 0.0 29 2.0497053548552396E-4 0.0 0.0 15.833153984114784 0.0 30 2.0497053548552396E-4 0.0 0.0 16.19933384575967 0.0 31 2.0497053548552396E-4 0.0 0.0 16.492134255700744 0.0 32 2.0497053548552396E-4 0.0 0.0 16.77847809377402 0.0 33 3.0745580322828596E-4 0.0 0.0 17.074763002818344 0.0 34 3.0745580322828596E-4 0.0 0.0 17.34727132974635 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTAAC 20 0.0012556753 40.000004 12 TCGTTAG 30 4.866759E-6 40.0 1 TATATCG 25 7.7937875E-5 40.0 1 CCGTAAG 30 4.866759E-6 40.0 1 CACGTCG 30 4.866759E-6 40.0 1 AATAGCG 30 4.866759E-6 40.0 1 ATTTCGC 25 7.7937875E-5 40.0 34 ACGCATT 555 0.0 38.918922 17 TACGCAT 565 0.0 38.58407 16 CGCATTT 570 0.0 37.894733 18 CGTTTTT 4070 0.0 37.10074 1 TCACCGC 585 0.0 36.923077 24 TTCACCG 585 0.0 36.923077 23 CTACGCA 605 0.0 36.36364 15 GCGTAAG 55 2.1645974E-10 36.363636 1 CTTGACG 50 3.423338E-9 36.0 1 CACCGCT 605 0.0 35.70248 25 TAAGACG 45 5.441143E-8 35.555557 1 GCCCGAA 45 5.441143E-8 35.555557 25 GCGATTA 160 0.0 35.000004 9 >>END_MODULE