FastQCFastQC Report
Sat 18 Jun 2016
SRR3553846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770626
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145541.8885944673551112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40460.5250277047491261No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA18000.23357633923589394No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC10490.13612309992136262No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC9130.11847510984576176No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC8640.11211664283322909No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC200.001255511440.033
GTTAACG200.001255511440.01
AATTGCG200.001255511440.01
CTAAACG257.7922625E-540.01
TATAGCG200.001255511440.01
CGATATT200.001255511440.010
TCGCGTT257.7922625E-540.013
AATCGCG200.001255511440.01
ACGCATT2800.038.5714317
CGCATTT2750.038.54545618
CGTTTTT50600.038.3794441
CGCTACA2800.037.85714328
TACGCAT2900.037.2413816
GTACGAG650.036.9230771
TCTAGCG455.4385964E-835.5555571
CCGCTAC3000.035.33333227
CTAACGG1450.034.4827582
AACGGGC3250.034.461544
CTAGGGA4650.034.40864
GCCGATA351.4004703E-534.2857139
Produced by FastQC (version 0.11.5)