##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	770626
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858442357252414	31.0	31.0	34.0	30.0	34.0
2	32.03090085203458	33.0	31.0	34.0	30.0	34.0
3	31.85775330705167	33.0	31.0	34.0	30.0	34.0
4	35.683687028467766	37.0	35.0	37.0	33.0	37.0
5	35.53358957522845	37.0	35.0	37.0	33.0	37.0
6	35.54557204143125	37.0	35.0	37.0	33.0	37.0
7	35.84917846010905	37.0	35.0	37.0	35.0	37.0
8	35.66738080469644	37.0	35.0	37.0	35.0	37.0
9	37.43651524864201	39.0	37.0	39.0	35.0	39.0
10	36.94895318870633	39.0	37.0	39.0	33.0	39.0
11	36.936100001816705	39.0	37.0	39.0	33.0	39.0
12	36.95071149948224	39.0	37.0	39.0	33.0	39.0
13	35.27155066141033	39.0	35.0	39.0	30.0	39.0
14	37.1368562700973	40.0	37.0	41.0	31.0	41.0
15	37.76768237770332	40.0	37.0	41.0	32.0	41.0
16	37.94061970398092	40.0	37.0	41.0	33.0	41.0
17	37.98381705262994	40.0	37.0	41.0	33.0	41.0
18	37.956613194986936	39.0	37.0	41.0	33.0	41.0
19	37.86000212813998	39.0	37.0	41.0	33.0	41.0
20	37.73754454171024	39.0	36.0	41.0	33.0	41.0
21	37.61133156680413	39.0	35.0	41.0	33.0	41.0
22	37.588974418200266	39.0	35.0	41.0	33.0	41.0
23	37.49305110390773	39.0	35.0	41.0	33.0	41.0
24	37.418991832614005	39.0	35.0	41.0	33.0	41.0
25	37.22728405218614	39.0	35.0	41.0	32.0	41.0
26	37.213987070251974	39.0	35.0	41.0	32.0	41.0
27	37.17946578495924	39.0	35.0	41.0	32.0	41.0
28	37.029030943674364	39.0	35.0	41.0	32.0	41.0
29	36.93537202222608	39.0	35.0	41.0	31.0	41.0
30	36.787573998281914	39.0	35.0	41.0	31.0	41.0
31	36.66290652015375	39.0	35.0	41.0	31.0	41.0
32	36.443300641296815	39.0	35.0	40.0	30.0	41.0
33	36.222728015924716	39.0	35.0	40.0	30.0	41.0
34	36.02300078118309	39.0	35.0	40.0	29.0	41.0
35	35.87197421317215	39.0	35.0	40.0	28.0	41.0
36	35.68442668687535	38.0	35.0	40.0	27.0	41.0
37	35.60629540140094	38.0	35.0	40.0	27.0	41.0
38	35.450019853988834	38.0	35.0	40.0	26.0	41.0
39	35.33528715615616	38.0	35.0	40.0	26.0	41.0
40	35.192936651501505	38.0	34.0	40.0	25.0	41.0
41	35.03774853171318	38.0	34.0	40.0	25.0	41.0
42	34.96002860012509	38.0	34.0	40.0	24.0	41.0
43	34.88768351963209	38.0	34.0	40.0	24.0	41.0
44	34.79242979084537	38.0	34.0	40.0	24.0	41.0
45	34.714132406640836	37.0	34.0	40.0	24.0	41.0
46	33.77643759748568	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	17.0
10	19.0
11	19.0
12	28.0
13	9.0
14	23.0
15	24.0
16	69.0
17	125.0
18	212.0
19	427.0
20	762.0
21	1149.0
22	1913.0
23	2772.0
24	3877.0
25	5367.0
26	7966.0
27	10718.0
28	13873.0
29	15470.0
30	17231.0
31	20277.0
32	25514.0
33	33271.0
34	49025.0
35	64022.0
36	77491.0
37	105855.0
38	153387.0
39	159704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.94172789394596	23.201397305567163	22.27137418150958	23.5855006189773
2	30.609919727598083	25.64953687002515	21.935802840807344	21.804740561569425
3	27.084993239262626	25.219626641198197	26.95470954782216	20.740670571717022
4	23.973756400640518	27.15597449346376	25.527039056559214	23.343230049336512
5	20.878869905764923	31.189448578168914	24.113642674916235	23.81803884114992
6	20.52305528232891	38.29730634575007	24.27299364412828	16.90664472779273
7	79.61540358098482	5.5357592398906865	10.916319978822411	3.932517200302092
8	79.45735025810185	5.285702792275371	10.957195838188694	4.299751111434081
9	72.70997864074143	7.90759720019828	13.725205222766945	5.657218936293351
10	33.1173617292954	27.744067809806573	20.587807834150418	18.550762626747606
11	27.541115923937166	26.806907630939	26.411774323731617	19.24020212139222
12	22.901770768180675	24.036302953702574	32.54289369940801	20.51903257870874
13	21.847173596530613	25.588677257190906	33.23025695992609	19.333892186352394
14	20.95374409895332	25.589585609621267	31.654135728615433	21.802534562809974
15	20.317378339168414	25.371451261701527	32.7968430860106	21.514327313119463
16	22.21051456867534	24.439092374251583	32.38016884974034	20.97022420733274
17	21.712607672204157	25.074290252340305	30.203756426593444	23.009345648862094
18	22.300311694648247	24.70549916561341	30.406838077095767	22.58735106264258
19	24.170220054864487	26.36921152413752	28.711592912774808	20.748975508223186
20	25.31448458785455	24.571063005919864	28.24781411475865	21.866638291466938
21	26.15302364571141	25.969406690145412	27.76521944497071	20.112350219172466
22	24.31776244248183	24.11429149808078	28.3102309031878	23.257715156249596
23	23.064625382481253	26.2528126484183	28.857578124797246	21.824983844303205
24	21.924772847010093	25.77488950541508	30.352077402008238	21.94826024556659
25	23.399937193917673	26.531157786007743	27.921326298360032	22.147578721714552
26	22.066346061513624	26.40917903107344	28.422996369185572	23.10147853822736
27	23.35244333827304	25.333170694993424	28.687586455686677	22.626799511046862
28	22.291617464243355	26.588903047652167	29.188737467980573	21.9307420201239
29	22.648599969375546	26.981441062201377	27.520742876570477	22.849216091852597
30	23.42225671077799	25.83146688536333	27.93949334696727	22.806783056891412
31	25.02835357229058	26.03052583224547	26.373753286289332	22.567367309174617
32	23.560326280192985	27.440288804167	26.847783490305282	22.15160142533473
33	23.386311907462247	26.877888885140134	26.22166913652018	23.514130070877442
34	23.160132152302154	26.815601861343897	26.98728047068228	23.036985515671674
35	24.53797302452811	25.87000698133725	27.00272246199843	22.589297532136214
36	24.38861393205005	28.09702761131859	25.760745160428016	21.753613296203348
37	23.993350860209752	27.666209030061275	26.822868680786787	21.517571428942183
38	23.880844923477795	27.016737042352577	27.94650063714435	21.155917397025277
39	24.25404800772359	25.240648511729425	27.331416277156496	23.17388720339049
40	24.594290875210543	25.29411154048786	29.93683057669998	20.174767007601613
41	22.14576201685383	25.799544785667756	29.692224243666836	22.362468953811575
42	22.736320861221916	26.659105714055848	28.31568101776997	22.28889240695227
43	22.723214633298124	25.419983234409425	29.12619091491852	22.730611217373927
44	23.66400822188714	25.42634170142196	27.830880349222582	23.07876972746832
45	23.648566230570992	24.85200343616748	28.05472434099031	23.44470599227122
46	23.260440213540683	25.726487297340086	27.43938045173664	23.573692037382596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4368.0
1	3457.5
2	2547.0
3	12057.5
4	21568.0
5	13851.0
6	6134.0
7	6134.0
8	5615.0
9	5096.0
10	4980.0
11	4864.0
12	4699.5
13	4535.0
14	4295.0
15	4055.0
16	3951.5
17	3848.0
18	3606.5
19	3365.0
20	3365.0
21	3291.5
22	3218.0
23	3716.0
24	4214.0
25	4917.5
26	5621.0
27	6450.0
28	7279.0
29	9300.0
30	11321.0
31	11321.0
32	12489.5
33	13658.0
34	15036.5
35	16415.0
36	18919.5
37	21424.0
38	23961.5
39	26499.0
40	29041.0
41	31583.0
42	34414.0
43	37245.0
44	37245.0
45	42397.0
46	47549.0
47	51567.5
48	55586.0
49	59096.0
50	62606.0
51	60921.5
52	59237.0
53	54639.0
54	50041.0
55	47955.0
56	45869.0
57	45869.0
58	44035.0
59	42201.0
60	40642.0
61	39083.0
62	37330.5
63	35578.0
64	31035.5
65	26493.0
66	23909.5
67	21326.0
68	18787.5
69	16249.0
70	16249.0
71	13983.0
72	11717.0
73	9451.0
74	7185.0
75	6157.0
76	5129.0
77	4048.0
78	2967.0
79	2353.0
80	1739.0
81	1739.0
82	1186.5
83	634.0
84	492.0
85	350.0
86	237.5
87	125.0
88	87.5
89	50.0
90	38.5
91	27.0
92	21.0
93	15.0
94	15.0
95	11.5
96	8.0
97	6.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	770626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.29568481827543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80086730196531	24.572504170016064
2	8.750536037005997	5.8271017975822526
3	3.4134007371656367	3.409545453094082
4	1.951383115288967	2.598905486654635
5	1.4198635820295566	2.3637665156101844
6	1.0461721428411255	2.089981076021876
7	0.91262454752269	2.1270521504215703
8	0.775289647100843	2.065103978619333
9	0.6735595189773924	2.0183962905198425
>10	7.131404909069506	44.75609863789621
>50	0.0820983658827705	1.819411051868456
>100	0.03501825966834278	2.2578108327310513
>500	0.006225468385483157	1.3155880770245476
>1k	0.001167275322278092	0.8932525643608327
>5k	0.0	0.0
>10k+	3.890917740926973E-4	1.8854819175790511
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14554	1.8885944673551112	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4046	0.5250277047491261	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	1800	0.23357633923589394	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC	1049	0.13612309992136262	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC	913	0.11847510984576176	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	864	0.11211664283322909	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2976463290882996E-4	0.0	0.0	0.08694230404891608	0.0
2	1.2976463290882996E-4	0.0	0.0	0.26731514379218974	0.0
3	1.2976463290882996E-4	0.0	0.0	0.49998313059772187	0.0
4	1.2976463290882996E-4	0.0	0.0	0.6993016067456846	0.0
5	1.2976463290882996E-4	0.0	0.0	1.160744641369484	0.0
6	1.2976463290882996E-4	0.0	0.0	2.0165423954032176	0.0
7	1.2976463290882996E-4	0.0	0.0	2.6212455847583653	0.0
8	1.2976463290882996E-4	0.0	0.0	3.647943360333028	0.0
9	1.2976463290882996E-4	0.0	0.0	4.176993768702328	0.0
10	1.2976463290882996E-4	0.0	0.0	4.974916496458723	0.0
11	1.2976463290882996E-4	0.0	0.0	5.711720082115059	0.0
12	1.2976463290882996E-4	0.0	0.0	6.305782571571683	0.0
13	1.2976463290882996E-4	0.0	0.0	6.637980031818288	0.0
14	1.2976463290882996E-4	0.0	0.0	6.806154996068131	0.0
15	1.2976463290882996E-4	0.0	0.0	6.984192072419046	0.0
16	1.2976463290882996E-4	0.0	0.0	7.318336002159284	0.0
17	1.2976463290882996E-4	0.0	0.0	7.672852979266207	0.0
18	1.2976463290882996E-4	0.0	0.0	8.146883183282164	0.0
19	1.2976463290882996E-4	0.0	0.0	8.351651774012296	0.0
20	1.2976463290882996E-4	0.0	0.0	8.572900473121852	0.0
21	1.2976463290882996E-4	0.0	0.0	8.829445152382608	0.0
22	1.2976463290882996E-4	0.0	0.0	9.081837363390283	0.0
23	1.2976463290882996E-4	0.0	0.0	9.354213327865917	0.0
24	1.2976463290882996E-4	0.0	0.0	9.58117167082346	0.0
25	1.2976463290882996E-4	0.0	0.0	9.780100853072696	0.0
26	1.2976463290882996E-4	0.0	0.0	9.962160633043785	0.0
27	1.2976463290882996E-4	0.0	0.0	10.17899733463444	0.0
28	1.2976463290882996E-4	0.0	0.0	10.381819455870941	0.0
29	1.2976463290882996E-4	0.0	0.0	10.591648867284519	0.0
30	1.2976463290882996E-4	0.0	0.0	10.877520353582671	0.0
31	1.2976463290882996E-4	0.0	0.0	11.09292964421133	0.0
32	2.595292658176599E-4	0.0	0.0	11.334162096788845	0.0
33	2.595292658176599E-4	0.0	0.0	11.553983384936402	0.0
34	3.892938987264899E-4	0.0	0.0	11.763942560982889	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAC	20	0.0012555114	40.0	33
GTTAACG	20	0.0012555114	40.0	1
AATTGCG	20	0.0012555114	40.0	1
CTAAACG	25	7.7922625E-5	40.0	1
TATAGCG	20	0.0012555114	40.0	1
CGATATT	20	0.0012555114	40.0	10
TCGCGTT	25	7.7922625E-5	40.0	13
AATCGCG	20	0.0012555114	40.0	1
ACGCATT	280	0.0	38.57143	17
CGCATTT	275	0.0	38.545456	18
CGTTTTT	5060	0.0	38.379444	1
CGCTACA	280	0.0	37.857143	28
TACGCAT	290	0.0	37.24138	16
GTACGAG	65	0.0	36.923077	1
TCTAGCG	45	5.4385964E-8	35.555557	1
CCGCTAC	300	0.0	35.333332	27
CTAACGG	145	0.0	34.482758	2
AACGGGC	325	0.0	34.46154	4
CTAGGGA	465	0.0	34.4086	4
GCCGATA	35	1.4004703E-5	34.285713	9
>>END_MODULE