FastQCFastQC Report
Sat 18 Jun 2016
SRR3553845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688294
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49290.7161184028917875No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA48270.7012991541405272No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35650.5179472725317961No Hit
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT15750.2288266351297556No Hit
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTT10670.15502096487838046RNA PCR Primer, Index 36 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT10670.15502096487838046No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8590.1248013203660064No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGT7120.10344416775389587No Hit
CGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC7030.10213658698172584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA304.8646634E-640.00000434
TAACACG304.8646634E-640.0000041
AACGTCC200.001255417940.026
GGTACGC257.791391E-540.08
CAACGTT200.001255417940.018
CAACGCG257.791391E-540.01
CGTTGAT700.040.025
CGTTCGG257.791391E-540.02
CGCTCGT257.791391E-540.013
CTACGCG401.9263098E-840.01
CGCTACA6600.039.6969728
TACGCAT6750.039.40740616
CGCATTT6850.038.8321218
CTACGCA6800.038.8235315
TCTACGC6950.038.56115314
CACCGCT6950.038.56115325
CGTTTTT15200.038.289481
CCGCTAC6850.038.24817727
TTCACCG7050.038.01418323
CGAATAT1000.038.014