Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553845_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 688294 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4929 | 0.7161184028917875 | No Hit |
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA | 4827 | 0.7012991541405272 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3565 | 0.5179472725317961 | No Hit |
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT | 1575 | 0.2288266351297556 | No Hit |
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTT | 1067 | 0.15502096487838046 | RNA PCR Primer, Index 36 (95% over 21bp) |
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT | 1067 | 0.15502096487838046 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 859 | 0.1248013203660064 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGT | 712 | 0.10344416775389587 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC | 703 | 0.10213658698172584 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCATTA | 30 | 4.8646634E-6 | 40.000004 | 34 |
TAACACG | 30 | 4.8646634E-6 | 40.000004 | 1 |
AACGTCC | 20 | 0.0012554179 | 40.0 | 26 |
GGTACGC | 25 | 7.791391E-5 | 40.0 | 8 |
CAACGTT | 20 | 0.0012554179 | 40.0 | 18 |
CAACGCG | 25 | 7.791391E-5 | 40.0 | 1 |
CGTTGAT | 70 | 0.0 | 40.0 | 25 |
CGTTCGG | 25 | 7.791391E-5 | 40.0 | 2 |
CGCTCGT | 25 | 7.791391E-5 | 40.0 | 13 |
CTACGCG | 40 | 1.9263098E-8 | 40.0 | 1 |
CGCTACA | 660 | 0.0 | 39.69697 | 28 |
TACGCAT | 675 | 0.0 | 39.407406 | 16 |
CGCATTT | 685 | 0.0 | 38.83212 | 18 |
CTACGCA | 680 | 0.0 | 38.82353 | 15 |
TCTACGC | 695 | 0.0 | 38.561153 | 14 |
CACCGCT | 695 | 0.0 | 38.561153 | 25 |
CGTTTTT | 1520 | 0.0 | 38.28948 | 1 |
CCGCTAC | 685 | 0.0 | 38.248177 | 27 |
TTCACCG | 705 | 0.0 | 38.014183 | 23 |
CGAATAT | 100 | 0.0 | 38.0 | 14 |