FastQCFastQC Report
Sat 18 Jun 2016
SRR3553843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459984
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA72691.5802723572993844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56091.2193902396605099No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20380.4430588890048349No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12360.26870499843472817No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT9090.19761556923719087No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT7880.1713103064454416No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT7590.16500573933006366No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATGAGTCGT7330.15935336881282827No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA5020.10913423075585237No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.10239486590837943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG304.861138E-640.0000041
CCGTAAG304.861138E-640.0000041
TTAGTGG304.861138E-640.0000042
TCGCCCT257.7873556E-540.028
TCGTTCA650.040.016
TCGCAAC200.00125498440.015
CGAAAGG401.9239451E-840.02
TCCGATC200.00125498440.015
CGTGCGG507.8216544E-1140.02
GCAACCC200.00125498440.022
TTCCGAT200.00125498440.014
GCGTTTA257.7873556E-540.032
CGACAGG200.00125498440.02
CGTTCAT650.040.017
TGGCCGT200.00125498440.020
ATCGTCC200.00125498440.035
TCTACGT353.0510455E-740.027
CTAAACG353.0510455E-740.01
TTAAACG200.00125498440.01
GACATAG257.7873556E-540.021