FastQCFastQC Report
Sat 18 Jun 2016
SRR3553842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087488
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116771.073758974811676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79490.7309505943973634No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA43650.4013837394067797No Hit
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT17680.1625765065913371RNA PCR Primer, Index 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTC15290.14059925258945385No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC14060.1292887829566855No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC12250.1126449211393597No Hit
GCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT11860.10905867467043316RNA PCR Primer, Index 40 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTT11820.10869085451977402TruSeq Adapter, Index 1 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11780.10832303436911488No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGC11770.10823107933145008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG353.056175E-740.01
GTCGCTA200.001255739140.022
ATCGTTC257.7943776E-540.017
AATTGCG507.8216544E-1140.01
AATAGCG555.456968E-1240.01
TATCGTT257.7943776E-540.016
ACGGTAT200.001255739140.05
ACGCATT5850.039.99999617
TACGCAT6200.038.7096816
CTACGCA6050.038.67768515
CGCATTT6100.038.03278718
TCACCGC6000.038.024
TCTACGC6400.037.812514
CGTTTTT40300.037.220841
CGCTACA6150.037.0731728
TTACGCG650.036.9230771
CCGCTAC6200.036.77419327
CGACGGT601.4551915E-1136.66666428
CGGTCTA601.4551915E-1136.66666431
TCGTAAG503.425157E-936.01
Produced by FastQC (version 0.11.5)