##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1087488
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.777242599458567	31.0	31.0	34.0	30.0	34.0
2	31.93870736964454	33.0	31.0	34.0	30.0	34.0
3	31.834935190089453	33.0	31.0	34.0	30.0	34.0
4	35.634964247881356	37.0	35.0	37.0	33.0	37.0
5	35.459293343926554	37.0	35.0	37.0	33.0	37.0
6	35.47882551347693	37.0	35.0	37.0	33.0	37.0
7	35.80968617584746	37.0	35.0	37.0	35.0	37.0
8	35.63543137947269	37.0	35.0	37.0	35.0	37.0
9	37.380569716631356	39.0	37.0	39.0	35.0	39.0
10	36.844612538253294	39.0	37.0	39.0	32.0	39.0
11	36.833466668137945	39.0	37.0	39.0	32.0	39.0
12	36.88245387535311	39.0	37.0	39.0	33.0	39.0
13	35.221308189147834	39.0	35.0	39.0	30.0	39.0
14	37.01707329184322	40.0	36.0	41.0	31.0	41.0
15	37.623736537782484	40.0	36.0	41.0	32.0	41.0
16	37.806343610228346	40.0	37.0	41.0	33.0	41.0
17	37.84150445798023	39.0	37.0	41.0	33.0	41.0
18	37.8509482403484	39.0	37.0	41.0	33.0	41.0
19	37.77525637064501	39.0	37.0	41.0	33.0	41.0
20	37.71633434116055	39.0	36.0	41.0	33.0	41.0
21	37.58655359875235	39.0	36.0	41.0	33.0	41.0
22	37.58786395803908	39.0	36.0	41.0	33.0	41.0
23	37.493881311793785	39.0	36.0	41.0	33.0	41.0
24	37.450923596398304	39.0	35.0	41.0	33.0	41.0
25	37.23971298993644	39.0	35.0	41.0	32.0	41.0
26	37.22554087953154	39.0	35.0	41.0	32.0	41.0
27	37.222646134945855	39.0	35.0	41.0	32.0	41.0
28	37.07868316707862	39.0	35.0	41.0	32.0	41.0
29	36.984823740583806	39.0	35.0	41.0	31.0	41.0
30	36.871345706803204	39.0	35.0	40.0	31.0	41.0
31	36.77105034722222	39.0	35.0	40.0	31.0	41.0
32	36.62696783780603	39.0	35.0	40.0	31.0	41.0
33	36.52203334657486	39.0	35.0	40.0	30.0	41.0
34	36.32377736581921	39.0	35.0	40.0	30.0	41.0
35	36.212938441619585	39.0	35.0	40.0	30.0	41.0
36	36.08690578654661	38.0	35.0	40.0	30.0	41.0
37	35.998371476282955	38.0	35.0	40.0	29.0	41.0
38	35.898774055437855	38.0	35.0	40.0	29.0	41.0
39	35.773704169609225	38.0	35.0	40.0	29.0	41.0
40	35.63468562411723	38.0	35.0	40.0	28.0	41.0
41	35.499186197916664	38.0	35.0	40.0	27.0	41.0
42	35.4417290121822	38.0	34.0	40.0	27.0	41.0
43	35.37050615730932	38.0	34.0	40.0	27.0	41.0
44	35.239698277130415	38.0	34.0	40.0	27.0	41.0
45	35.144640676494824	38.0	34.0	40.0	26.0	41.0
46	34.12756186734934	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	27.0
10	31.0
11	28.0
12	27.0
13	24.0
14	20.0
15	43.0
16	78.0
17	133.0
18	282.0
19	454.0
20	878.0
21	1472.0
22	2321.0
23	3281.0
24	4915.0
25	6895.0
26	9475.0
27	12645.0
28	16062.0
29	19055.0
30	22728.0
31	28032.0
32	36506.0
33	48910.0
34	72332.0
35	93032.0
36	113381.0
37	155442.0
38	223089.0
39	215878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.15033177377589	24.60477724811676	22.754366025188325	20.49052495291902
2	28.41116407721281	26.59633945386064	22.67013521068738	22.32236125823917
3	26.98641272363465	26.99156220574388	24.988137800141246	21.033887270480225
4	24.670709010122412	27.60894832862524	23.825918079096045	23.894424582156308
5	21.16621056967985	32.00715778013183	21.78194150188324	25.044690148305083
6	20.41631723752354	39.106362552966104	22.32732683027307	18.14999337923729
7	80.712614759887	6.032158515772128	8.907592543549905	4.34763418079096
8	80.46966955037664	5.478497233992467	8.989892302259888	5.061940913370997
9	73.20595721516008	8.508047904896422	12.141927083333332	6.1440677966101696
10	33.235493173258	28.8720427259887	18.71524099576271	19.177223104990585
11	26.57132768361582	28.170241878531073	24.845515536723166	20.412914901129945
12	22.94480490819209	23.73083657015066	31.95354799317326	21.37081052848399
13	21.97035737405838	25.93389536252354	31.792810587335218	20.302936676082865
14	20.69025129472693	26.58852327565913	30.23481638418079	22.486409045433145
15	19.999025276600754	25.98364303790019	32.22619467984934	21.79113700564972
16	21.737803083804145	25.42713114995292	30.8967087452919	21.938357020951035
17	20.865793461629	26.04212644185499	28.72132841925612	24.370751677259886
18	22.589950417843692	25.307497645951038	28.44803804731638	23.65451388888889
19	23.729181379472696	27.66274202565913	26.809215366054612	21.79886122881356
20	25.1611052259887	24.999540224811675	27.084712658898308	22.75464189030132
21	25.742720839218457	27.51423463983051	26.22033530484934	20.522709216101696
22	23.5774555673258	24.871630767419962	26.60056938559322	24.950344279661017
23	22.07068032015066	27.93814736346516	26.916802760122412	23.07436955626177
24	22.04189839336158	26.15063338629944	28.69227062735405	23.115197592984934
25	22.934138123822976	26.865767714218457	26.35992305202448	23.840171109934087
26	20.772643008474574	27.19965645597928	27.83442208097928	24.193278454566855
27	22.56585819797552	25.03089689265537	27.737961246468927	24.66528366290019
28	21.867183821798495	26.970688412193972	27.93640021774953	23.225727548258003
29	23.119611434792844	26.66631723752354	26.330221574858758	23.883849752824858
30	23.1983249470339	24.92229799317326	27.87221560145951	24.007161458333336
31	23.356119791666664	27.17685160663842	25.33177377589454	24.135254825800377
32	22.837217514124294	27.81382415254237	26.32948593455744	23.019472398775896
33	22.69128486935028	26.01251691972693	24.74988229755179	26.546315913371
34	22.38479872881356	27.1207590336629	26.67247822504708	23.82196401247646
35	23.46269568032015	25.6916857933145	26.664294226694917	24.181324299670433
36	23.682927995527304	28.2145642066855	24.686249411487758	23.416258386299436
37	22.87979269656309	28.33953110287194	25.747318591101692	23.033357609463277
38	23.487431585451976	27.314048522834273	26.54815501412429	22.650364877589453
39	24.34482035663842	25.247726871468927	25.165059292608287	25.24239347928437
40	23.40577551200565	24.953102930790962	29.60575197151601	22.03536958568738
41	20.53530705626177	26.24074932321092	28.71213291548964	24.511810705037664
42	21.605387829566855	26.29822122175141	27.773547846045197	24.322843102636536
43	21.759412517655367	24.71218073210923	28.35856579566855	25.16984095456685
44	23.880539371468927	24.986666519538606	26.67413341572505	24.45866069326742
45	23.965689736346516	23.78867628884181	27.54862582391714	24.69700815089454
46	23.716859404425612	25.65012211629002	26.1979902306968	24.43502824858757
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8455.0
1	6349.5
2	4244.0
3	11192.5
4	18141.0
5	11772.0
6	5403.0
7	5403.0
8	5131.5
9	4860.0
10	4807.5
11	4755.0
12	4660.0
13	4565.0
14	4537.5
15	4510.0
16	4155.5
17	3801.0
18	3999.0
19	4197.0
20	4197.0
21	4117.5
22	4038.0
23	4474.5
24	4911.0
25	6102.5
26	7294.0
27	8475.0
28	9656.0
29	11640.5
30	13625.0
31	13625.0
32	15915.5
33	18206.0
34	20536.5
35	22867.0
36	24126.0
37	25385.0
38	28205.5
39	31026.0
40	37119.5
41	43213.0
42	48556.0
43	53899.0
44	53899.0
45	66020.0
46	78141.0
47	86112.0
48	94083.0
49	98474.5
50	102866.0
51	98994.0
52	95122.0
53	84431.0
54	73740.0
55	70020.0
56	66300.0
57	66300.0
58	63221.5
59	60143.0
60	56206.5
61	52270.0
62	49217.5
63	46165.0
64	41431.0
65	36697.0
66	32163.0
67	27629.0
68	23994.0
69	20359.0
70	20359.0
71	17285.0
72	14211.0
73	11889.0
74	9567.0
75	7930.5
76	6294.0
77	5038.0
78	3782.0
79	2636.0
80	1490.0
81	1490.0
82	1189.0
83	888.0
84	629.5
85	371.0
86	283.5
87	196.0
88	129.5
89	63.0
90	52.5
91	42.0
92	24.5
93	7.0
94	7.0
95	7.0
96	7.0
97	4.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1087488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.501962928782557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.04551010303082	23.640808772372534
2	9.75160470473985	6.143893798097126
3	3.6110407723133884	3.412646176312161
4	1.8448925192566108	2.3247094299684004
5	1.1194600034683055	1.7632593764756677
6	0.7560572995533891	1.4290373413539785
7	0.6157293866818951	1.3577679019390556
8	0.5116197460299027	1.2893621018453714
9	0.4269240995930287	1.2104052442905113
>10	5.866699263049681	42.62176040644026
>50	0.3893779616139892	7.617968582067573
>100	0.05355718494716796	3.0349664679650172
>500	0.003763477860970659	0.8049104253584269
>1k	0.0031844812669751727	1.5586589711988301
>5k	2.89498296997743E-4	0.7249300896413025
>10k+	2.89498296997743E-4	1.0649149146737311
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11677	1.073758974811676	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7949	0.7309505943973634	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	4365	0.4013837394067797	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT	1768	0.1625765065913371	RNA PCR Primer, Index 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTC	1529	0.14059925258945385	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	1406	0.1292887829566855	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC	1225	0.1126449211393597	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT	1186	0.10905867467043316	RNA PCR Primer, Index 40 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTT	1182	0.10869085451977402	TruSeq Adapter, Index 1 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1178	0.10832303436911488	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGC	1177	0.10823107933145008	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.195503766478342E-5	0.0	0.0	0.12027718926553672	0.0
2	9.195503766478342E-5	0.0	0.0	0.506580302495292	0.0
3	9.195503766478342E-5	0.0	0.0	0.8263999234934086	0.0
4	9.195503766478342E-5	0.0	0.0	1.1270009416195856	0.0
5	1.8391007532956685E-4	0.0	0.0	1.9771252648305084	0.0
6	1.8391007532956685E-4	0.0	0.0	3.2267942266949152	0.0
7	1.8391007532956685E-4	0.0	0.0	4.081424346751413	0.0
8	1.8391007532956685E-4	0.0	0.0	5.6025445798022595	0.0
9	1.8391007532956685E-4	0.0	0.0	6.343610228342749	0.0
10	1.8391007532956685E-4	0.0	0.0	7.552635063559322	0.0
11	1.8391007532956685E-4	0.0	0.0	8.690302789548022	0.0
12	1.8391007532956685E-4	0.0	0.0	9.631462600047081	0.0
13	1.8391007532956685E-4	0.0	0.0	10.145307350517891	0.0
14	1.8391007532956685E-4	0.0	0.0	10.407838983050848	0.0
15	1.8391007532956685E-4	0.0	0.0	10.687290342514125	0.0
16	1.8391007532956685E-4	0.0	0.0	11.239940118879472	0.0
17	1.8391007532956685E-4	0.0	0.0	11.853648040254237	0.0
18	1.8391007532956685E-4	0.0	0.0	12.6676340336629	0.0
19	1.8391007532956685E-4	0.0	0.0	13.029017331685498	0.0
20	1.8391007532956685E-4	0.0	0.0	13.437665519067796	0.0
21	4.597751883239171E-4	0.0	0.0	13.859279366760829	0.0
22	4.597751883239171E-4	0.0	0.0	14.294226694915254	0.0
23	4.597751883239171E-4	0.0	0.0	14.761910016478343	0.0
24	4.597751883239171E-4	0.0	0.0	15.124672640065913	0.0
25	4.597751883239171E-4	0.0	0.0	15.457274011299434	0.0
26	4.597751883239171E-4	0.0	0.0	15.757783074387948	0.0
27	4.597751883239171E-4	0.0	0.0	16.10555702683616	0.0
28	4.597751883239171E-4	0.0	0.0	16.442480284839924	0.0
29	4.597751883239171E-4	0.0	0.0	16.7741621056968	0.0
30	4.597751883239171E-4	0.0	0.0	17.2021208509887	0.0
31	4.597751883239171E-4	0.0	0.0	17.53545786252354	0.0
32	4.597751883239171E-4	0.0	0.0	17.90548493408663	0.0
33	4.597751883239171E-4	0.0	0.0	18.250868055555557	0.0
34	4.597751883239171E-4	0.0	0.0	18.568020980461394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	3.056175E-7	40.0	1
GTCGCTA	20	0.0012557391	40.0	22
ATCGTTC	25	7.7943776E-5	40.0	17
AATTGCG	50	7.8216544E-11	40.0	1
AATAGCG	55	5.456968E-12	40.0	1
TATCGTT	25	7.7943776E-5	40.0	16
ACGGTAT	20	0.0012557391	40.0	5
ACGCATT	585	0.0	39.999996	17
TACGCAT	620	0.0	38.70968	16
CTACGCA	605	0.0	38.677685	15
CGCATTT	610	0.0	38.032787	18
TCACCGC	600	0.0	38.0	24
TCTACGC	640	0.0	37.8125	14
CGTTTTT	4030	0.0	37.22084	1
CGCTACA	615	0.0	37.07317	28
TTACGCG	65	0.0	36.923077	1
CCGCTAC	620	0.0	36.774193	27
CGACGGT	60	1.4551915E-11	36.666664	28
CGGTCTA	60	1.4551915E-11	36.666664	31
TCGTAAG	50	3.425157E-9	36.0	1
>>END_MODULE