FastQCFastQC Report
Sat 18 Jun 2016
SRR3553841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370385
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79412.143985312580153No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.9881609676417781No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA31190.8420967371788814No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10630.28699866355278963No Hit
CCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT8970.24218043387286203No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTC8960.24191044453744076No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCC5800.1565938145443255No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCG5570.15038405982963673No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5340.14417430511494794No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGC5320.14363432644410545No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCGTATCGT4760.1285149236605154No Hit
GCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT3800.10259594746007533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG200.00125466740.0000041
TAGCCGA200.00125466740.00000418
CTACGCG200.00125466740.0000041
AAGCACG401.922308E-840.0000041
GTACTCG200.00125466740.00000415
CTCGCAT200.00125466740.00000421
CGTCGTC200.00125466740.00000415
ACGTAAG200.00125466740.0000041
GCTACGA200.00125466740.00000410
CGCGTTT200.00125466740.00000422
CAGTAAT200.00125466740.00000411
GATTACC200.00125466740.00000430
GGTTACA401.922308E-840.0000048
ACGACCA800.040.00000428
CACGACC750.040.027
ATGTCGG257.784413E-540.033
CGTTCAG304.8585716E-640.038
TATTGCG257.784413E-540.01
CCCGTAA257.784413E-540.033
ACTACGG257.784413E-540.02