FastQCFastQC Report
Sat 18 Jun 2016
SRR3553837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558211
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107871.932423402620156No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65161.1673005368937552No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA36450.6529788914944349No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12790.22912482914166865No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC11550.20691100676984153No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT11240.20135755117688475No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT10720.19204207727902173No Hit
CGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTC10560.1891757776181408No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC9730.17430684812732103No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGT8740.15657161897562033No Hit
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT8470.15173473829788378No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATG6720.1203845857569987No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCG6490.11626427999448237No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT6420.11501027389284697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG257.789496E-540.01
GTCGCTA353.0526098E-740.022
TCGTTCA353.0526098E-740.016
CACGACC1200.040.027
CCTAACG200.001255214240.01
CCGTATT200.001255214240.016
CTAGTAG200.001255214240.01
TGCGACG257.789496E-540.01
ACTAACG304.86301E-640.01
CGTACGG451.2187229E-940.02
TCCGTAT200.001255214240.015
CGTACAT200.001255214240.035
TCGTAAG304.86301E-640.01
ATGCGCT200.001255214240.012
TTACGCG304.86301E-640.01
TTACGAG304.86301E-640.01
CGGTCTA200.001255214240.031
TTGCACG304.86301E-640.01
GGTTCGC257.789496E-540.010
GCATGCG451.2187229E-940.01