##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	558211
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62406330222801	31.0	31.0	34.0	30.0	34.0
2	31.78868743181342	31.0	31.0	34.0	30.0	34.0
3	31.661212337270314	31.0	31.0	34.0	30.0	34.0
4	35.49482901626804	37.0	35.0	37.0	33.0	37.0
5	35.31567991315112	37.0	35.0	37.0	33.0	37.0
6	35.32501688429644	37.0	35.0	37.0	32.0	37.0
7	35.603293378310354	37.0	35.0	37.0	35.0	37.0
8	35.351146788579946	37.0	35.0	37.0	33.0	37.0
9	37.136500355600305	39.0	37.0	39.0	34.0	39.0
10	36.64639535946085	39.0	35.0	39.0	32.0	39.0
11	36.63357941710213	39.0	35.0	39.0	32.0	39.0
12	36.711718328732324	39.0	35.0	39.0	32.0	39.0
13	35.101105137663	39.0	35.0	39.0	30.0	39.0
14	36.84668163113948	39.0	36.0	41.0	30.0	41.0
15	37.4187520489564	39.0	36.0	41.0	32.0	41.0
16	37.57813622447426	39.0	36.0	41.0	32.0	41.0
17	37.57668336883365	39.0	36.0	41.0	32.0	41.0
18	37.52469048442256	39.0	36.0	41.0	33.0	41.0
19	37.38676235330368	39.0	36.0	41.0	32.0	41.0
20	37.290581876745534	39.0	35.0	41.0	32.0	41.0
21	37.13644661248166	39.0	35.0	40.0	32.0	41.0
22	37.11721195031986	39.0	35.0	40.0	32.0	41.0
23	37.00705647147763	39.0	35.0	40.0	31.0	41.0
24	36.96240847994755	39.0	35.0	40.0	31.0	41.0
25	36.73474188075835	39.0	35.0	40.0	31.0	41.0
26	36.723332216670755	39.0	35.0	40.0	31.0	41.0
27	36.70197470132262	39.0	35.0	40.0	31.0	41.0
28	36.54031002613707	39.0	35.0	40.0	31.0	41.0
29	36.45056797519218	38.0	35.0	40.0	30.0	41.0
30	36.284134493945835	38.0	35.0	40.0	30.0	41.0
31	36.20194155973279	38.0	35.0	40.0	30.0	41.0
32	36.02822588591052	38.0	35.0	40.0	30.0	41.0
33	35.842677768800684	38.0	35.0	40.0	29.0	41.0
34	35.65523072816551	38.0	35.0	40.0	27.0	41.0
35	35.541316813892955	38.0	35.0	40.0	27.0	41.0
36	35.36210680190824	38.0	34.0	40.0	26.0	41.0
37	35.25970645508598	38.0	34.0	40.0	25.0	41.0
38	35.06440396194271	38.0	34.0	40.0	25.0	41.0
39	34.87981784665655	38.0	34.0	40.0	24.0	41.0
40	34.683006963316735	38.0	34.0	40.0	23.0	41.0
41	34.50610790543361	38.0	33.0	40.0	23.0	41.0
42	34.424545557145954	37.0	33.0	40.0	23.0	41.0
43	34.35761029431523	37.0	33.0	40.0	22.0	41.0
44	34.21958184270822	37.0	33.0	40.0	22.0	41.0
45	34.13787797087481	37.0	33.0	40.0	22.0	41.0
46	33.130201662095516	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	23.0
11	10.0
12	13.0
13	13.0
14	10.0
15	23.0
16	59.0
17	124.0
18	252.0
19	475.0
20	905.0
21	1424.0
22	2064.0
23	2900.0
24	3960.0
25	5418.0
26	6952.0
27	8630.0
28	10466.0
29	12224.0
30	14167.0
31	17159.0
32	21008.0
33	26960.0
34	38207.0
35	49386.0
36	57023.0
37	75537.0
38	106872.0
39	95939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.276544174156367	25.996621349274733	21.422007090508785	22.30482738606011
2	27.46452506310338	28.745940155245957	22.62836096028204	21.161173821368624
3	25.104664723554354	29.472726263008077	25.300468819138285	20.122140194299288
4	22.544163407743667	30.1147773870454	23.5726275548135	23.76843165039743
5	20.386198050557944	34.13995782956624	22.062445920986868	23.41139819888895
6	19.70383958753948	39.71974755065737	23.2041289046615	17.372283957141654
7	75.74447655098162	9.216228272104992	9.98833774325479	5.050957433658598
8	75.18662297948266	8.853103933817142	10.040110280879452	5.9201628058207385
9	68.09020245032792	12.054223223834715	13.318082230554396	6.537492095282967
10	31.424497188339178	30.279768761274862	19.782662828213702	18.51307122217226
11	24.77718998729871	31.419481163932634	25.306022274731237	18.497306574037417
12	20.812022693927563	26.52276648077519	31.710052292054435	20.955158533242805
13	21.15902409662296	28.322623524079603	31.24732404055097	19.27102833874646
14	19.913258606512592	29.025583515910654	30.16959536805975	20.891562509517012
15	18.768888466905885	29.126441435227896	31.40299994088257	20.70167015698365
16	20.377957439032908	28.927412752525477	30.212410719244158	20.482219089197454
17	19.563390904156314	29.253454338950686	28.06071539256661	23.122439364326393
18	21.293740180684363	27.38211894785305	28.989038195234418	22.335102676228164
19	22.796218634172384	29.771358858926106	26.89180256211361	20.540619944787903
20	23.28958046330151	28.1594235871382	27.003946536345573	21.547049413214715
21	23.227775876863767	30.730494382948383	27.143141213627104	18.898588526560744
22	21.980935524380566	27.913280103760048	26.691340729580748	23.414443642278638
23	19.705451881098725	30.439027536182557	27.39985417700475	22.455666405713966
24	19.733756590249925	28.05390793087202	28.976139846760457	23.236195632117603
25	20.491534563095318	29.61245837147602	26.65909485839584	23.236912207032827
26	19.82404502956767	29.637538493508725	27.180761396676168	23.357655080247433
27	20.87418556782292	28.117145667140203	26.711046539749304	24.297622225287572
28	19.94048845329096	29.28068418572905	27.607840046147423	23.170987314832566
29	22.068178520308628	28.206000956627513	25.955418291649572	23.770402231414288
30	21.3338683759367	27.091368676002443	27.320672648872918	24.25409029918794
31	21.203093453909005	28.424198018312076	26.50986813230123	23.862840395477697
32	21.716340236935498	28.26296866238752	26.202994924858164	23.81769617581882
33	21.004602202393002	27.073096015664326	26.938200787874116	24.98410099406855
34	20.502820618010038	26.97510439600796	27.98887875731578	24.53319622866622
35	21.45156580576162	25.853485509959494	28.25759435052337	24.437354333755515
36	21.585207027450192	26.962564334991608	28.26995526781092	23.182273369747282
37	22.56386921791222	26.415459387220963	29.315796356574843	21.70487503829197
38	23.25124370533723	27.196884332268624	28.796279543040175	20.75559241935397
39	24.387194089690098	24.686364116794547	28.163723036629516	22.762718756885835
40	23.00259220975581	25.4710136489607	31.00870459378264	20.517689547500854
41	21.294456755599587	25.95559743537838	29.903566930784237	22.8463788782378
42	22.453874968425918	25.52242789912775	29.463231645381406	22.560465487064928
43	22.15739209725355	24.29403935071147	30.766681416167007	22.78188713586798
44	22.890089948066233	24.966724052374463	27.87512248952457	24.268063510034736
45	22.173335889117197	23.12799281991935	30.557620684651503	24.14105060631195
46	23.10667471619155	24.537137390699932	28.727846638636645	23.628341254471874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11458.0
1	7927.0
2	4396.0
3	7696.0
4	10996.0
5	7547.5
6	4099.0
7	4099.0
8	4017.5
9	3936.0
10	4049.0
11	4162.0
12	4193.0
13	4224.0
14	4034.0
15	3844.0
16	3566.0
17	3288.0
18	3355.5
19	3423.0
20	3423.0
21	3168.5
22	2914.0
23	3033.0
24	3152.0
25	3562.5
26	3973.0
27	4195.0
28	4417.0
29	6004.0
30	7591.0
31	7591.0
32	8133.5
33	8676.0
34	10063.5
35	11451.0
36	12703.0
37	13955.0
38	15686.5
39	17418.0
40	20115.0
41	22812.0
42	25690.5
43	28569.0
44	28569.0
45	34658.0
46	40747.0
47	45521.0
48	50295.0
49	51893.5
50	53492.0
51	49349.5
52	45207.0
53	40011.5
54	34816.0
55	32046.0
56	29276.0
57	29276.0
58	27810.0
59	26344.0
60	25240.0
61	24136.0
62	21908.0
63	19680.0
64	17153.5
65	14627.0
66	13562.0
67	12497.0
68	10740.0
69	8983.0
70	8983.0
71	7871.0
72	6759.0
73	5540.0
74	4321.0
75	3815.5
76	3310.0
77	2788.5
78	2267.0
79	1792.0
80	1317.0
81	1317.0
82	1012.0
83	707.0
84	562.5
85	418.0
86	265.0
87	112.0
88	90.5
89	69.0
90	64.5
91	60.0
92	35.0
93	10.0
94	10.0
95	8.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	558211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.230506309461845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45629771586945	26.935940790770324
2	9.222872476480712	6.4985293394803145
3	3.4534475388404386	3.6500011591954067
4	1.7978456658541073	2.5335605229724703
5	1.134540644591612	1.9985220668812846
6	0.8266455327799188	1.747388439497483
7	0.6643635915678279	1.6384105993154965
8	0.5239441424976582	1.4767053934455459
9	0.46832732255131454	1.4849467822833713
>10	5.214515559916448	39.615240781081745
>50	0.19416571842366173	4.281213767627888
>100	0.03534943158635862	2.401053404331538
>500	0.0035861742189059473	0.9313264351161945
>1k	0.003073863616205097	1.6841486368351182
>5k	5.123106027008496E-4	1.1760703587630084
>10k+	5.123106027008496E-4	1.9469415224027886
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10787	1.932423402620156	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6516	1.1673005368937552	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	3645	0.6529788914944349	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1279	0.22912482914166865	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	1155	0.20691100676984153	No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	1124	0.20135755117688475	No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	1072	0.19204207727902173	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTC	1056	0.1891757776181408	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	973	0.17430684812732103	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGT	874	0.15657161897562033	No Hit
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	847	0.15173473829788378	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATG	672	0.1203845857569987	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCG	649	0.11626427999448237	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	642	0.11501027389284697	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7914372880505758E-4	0.0	0.0	0.21156874371877302	0.0
2	1.7914372880505758E-4	0.0	0.0	0.7264278203045085	0.0
3	1.7914372880505758E-4	0.0	0.0	1.1436535646914876	0.0
4	1.7914372880505758E-4	0.0	0.0	1.5728819389084057	0.0
5	1.7914372880505758E-4	0.0	0.0	2.589164312419497	0.0
6	1.7914372880505758E-4	0.0	0.0	3.9155444804921435	0.0
7	1.7914372880505758E-4	0.0	0.0	4.969267893323492	0.0
8	1.7914372880505758E-4	0.0	0.0	6.797429645779105	0.0
9	1.7914372880505758E-4	0.0	0.0	7.7302310416670394	0.0
10	1.7914372880505758E-4	0.0	0.0	9.250265580577954	0.0
11	1.7914372880505758E-4	0.0	0.0	10.59223125305664	0.0
12	1.7914372880505758E-4	0.0	0.0	11.758098640119954	0.0
13	1.7914372880505758E-4	0.0	0.0	12.415914412292127	0.0
14	1.7914372880505758E-4	0.0	0.0	12.731028231260222	0.0
15	1.7914372880505758E-4	0.0	0.0	13.059040398702283	0.0
16	1.7914372880505758E-4	0.0	0.0	13.70574925968854	0.0
17	1.7914372880505758E-4	0.0	0.0	14.403693227113045	0.0
18	1.7914372880505758E-4	0.0	0.0	15.375727099609287	0.0
19	1.7914372880505758E-4	0.0	0.0	15.731327401287327	0.0
20	1.7914372880505758E-4	0.0	0.0	16.17022953685972	0.0
21	1.7914372880505758E-4	0.0	0.0	16.602682498195126	0.0
22	1.7914372880505758E-4	0.0	0.0	17.03244830359846	0.0
23	1.7914372880505758E-4	0.0	0.0	17.501625729338905	0.0
24	1.7914372880505758E-4	0.0	0.0	17.85597202491531	0.0
25	1.7914372880505758E-4	0.0	0.0	18.182730186255736	0.0
26	1.7914372880505758E-4	0.0	0.0	18.469718439801436	0.0
27	3.5828745761011516E-4	0.0	0.0	18.808479230971802	0.0
28	3.5828745761011516E-4	0.0	0.0	19.120368462821407	0.0
29	3.5828745761011516E-4	0.0	0.0	19.443006318399316	0.0
30	3.5828745761011516E-4	0.0	0.0	19.876534142107555	0.0
31	3.5828745761011516E-4	0.0	0.0	20.190393954974017	0.0
32	5.374311864151728E-4	0.0	0.0	20.52037670343293	0.0
33	5.374311864151728E-4	0.0	0.0	20.82993706680807	0.0
34	5.374311864151728E-4	0.0	0.0	21.112805014591256	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	25	7.789496E-5	40.0	1
GTCGCTA	35	3.0526098E-7	40.0	22
TCGTTCA	35	3.0526098E-7	40.0	16
CACGACC	120	0.0	40.0	27
CCTAACG	20	0.0012552142	40.0	1
CCGTATT	20	0.0012552142	40.0	16
CTAGTAG	20	0.0012552142	40.0	1
TGCGACG	25	7.789496E-5	40.0	1
ACTAACG	30	4.86301E-6	40.0	1
CGTACGG	45	1.2187229E-9	40.0	2
TCCGTAT	20	0.0012552142	40.0	15
CGTACAT	20	0.0012552142	40.0	35
TCGTAAG	30	4.86301E-6	40.0	1
ATGCGCT	20	0.0012552142	40.0	12
TTACGCG	30	4.86301E-6	40.0	1
TTACGAG	30	4.86301E-6	40.0	1
CGGTCTA	20	0.0012552142	40.0	31
TTGCACG	30	4.86301E-6	40.0	1
GGTTCGC	25	7.789496E-5	40.0	10
GCATGCG	45	1.2187229E-9	40.0	1
>>END_MODULE