FastQCFastQC Report
Sat 18 Jun 2016
SRR3553833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852071
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59640.6999416715273727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51100.5997152819424673No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC13800.161958334458044No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT10020.11759583414997106No Hit
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCT9540.11196250077751738No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9230.10832430630780768No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG200.00125558640.01
CCTAGCG257.7929544E-540.01
CGCGCGA200.00125558640.033
CGCGACA200.00125558640.017
ATCGTAG257.7929544E-540.01
CCGTAAG304.8660313E-640.01
ATTCACG200.00125558640.01
TCGCTAG257.7929544E-540.01
TGTCGTC200.00125558640.016
TATCGCG304.8660313E-640.01
TCGGTAC257.7929544E-540.06
TATACGG900.037.777782
CGTTTTT27800.036.474821
TAAGGGT2700.036.2962954
TATTGCG503.423338E-936.01
CGTAAGG900.035.5555572
CGGTCTA455.4396878E-835.55555731
GCGATAC1300.035.3846139
CGTGTAC1700.035.29411718
ACACGAC2400.035.026
Produced by FastQC (version 0.11.5)