##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509605
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71448474799109	31.0	31.0	34.0	30.0	34.0
2	31.8685059997449	33.0	31.0	34.0	30.0	34.0
3	31.828824285476006	33.0	31.0	34.0	30.0	34.0
4	35.59502555901139	37.0	35.0	37.0	33.0	37.0
5	35.39598708803877	37.0	35.0	37.0	33.0	37.0
6	35.417440959174264	37.0	35.0	37.0	33.0	37.0
7	35.797025146927524	37.0	35.0	37.0	35.0	37.0
8	35.64163028227745	37.0	35.0	37.0	35.0	37.0
9	37.39887363742506	39.0	37.0	39.0	35.0	39.0
10	36.74055199615388	39.0	37.0	39.0	32.0	39.0
11	36.712449838600484	39.0	37.0	39.0	32.0	39.0
12	36.808704781154034	39.0	37.0	39.0	32.0	39.0
13	35.13045986597463	39.0	35.0	39.0	30.0	39.0
14	36.92963373593273	39.0	36.0	41.0	30.0	41.0
15	37.55919584776444	40.0	36.0	41.0	32.0	41.0
16	37.74424897714897	39.0	37.0	41.0	33.0	41.0
17	37.8134260849089	39.0	37.0	41.0	33.0	41.0
18	37.820335357777104	39.0	37.0	41.0	33.0	41.0
19	37.80006083142826	39.0	37.0	41.0	33.0	41.0
20	37.76972164715809	39.0	36.0	41.0	33.0	41.0
21	37.64942651661581	39.0	36.0	41.0	33.0	41.0
22	37.655866798795145	39.0	36.0	41.0	33.0	41.0
23	37.56386809391588	39.0	36.0	41.0	32.0	41.0
24	37.5369492057574	39.0	36.0	41.0	32.0	41.0
25	37.340553958458024	39.0	36.0	41.0	32.0	41.0
26	37.317532206316656	39.0	36.0	41.0	32.0	41.0
27	37.30129021497042	39.0	36.0	41.0	32.0	41.0
28	37.18301429538564	39.0	35.0	41.0	32.0	41.0
29	37.130008536023	39.0	35.0	41.0	31.0	41.0
30	36.98852837001207	39.0	35.0	41.0	31.0	41.0
31	36.915163705222675	39.0	35.0	41.0	31.0	41.0
32	36.82511749296023	39.0	35.0	41.0	31.0	41.0
33	36.66866494638004	39.0	35.0	40.0	30.0	41.0
34	36.60042778230198	39.0	35.0	40.0	30.0	41.0
35	36.524594538907586	39.0	35.0	40.0	30.0	41.0
36	36.375422140677586	39.0	35.0	40.0	30.0	41.0
37	36.31233798726465	39.0	35.0	40.0	30.0	41.0
38	36.256094426075094	39.0	35.0	40.0	30.0	41.0
39	36.14103472297171	39.0	35.0	40.0	29.0	41.0
40	36.0885116904269	39.0	35.0	40.0	29.0	41.0
41	35.934074430195935	39.0	35.0	40.0	29.0	41.0
42	35.8455293805987	38.0	35.0	40.0	28.0	41.0
43	35.76389360386966	38.0	35.0	40.0	28.0	41.0
44	35.679100479783365	38.0	35.0	40.0	28.0	41.0
45	35.619495491606244	38.0	35.0	40.0	28.0	41.0
46	34.650331138823205	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	13.0
11	14.0
12	11.0
13	3.0
14	13.0
15	16.0
16	27.0
17	45.0
18	139.0
19	233.0
20	395.0
21	681.0
22	1052.0
23	1608.0
24	2202.0
25	3113.0
26	4191.0
27	5502.0
28	6850.0
29	8312.0
30	10434.0
31	13069.0
32	16753.0
33	21741.0
34	31838.0
35	40740.0
36	51355.0
37	72033.0
38	108185.0
39	109020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.53225537426046	23.302557863443255	22.352213969643156	21.81297279265313
2	25.479341843192277	26.89102343972292	23.77252970437888	23.85710501270592
3	26.841377145043708	27.77052815415861	23.985635933713368	21.40245876708431
4	23.95443529792683	29.22067091178462	23.112410592517733	23.712483197770823
5	21.151676298309475	33.17216275350516	21.38754525563917	24.288615692546188
6	19.757851669430245	40.243129482638516	21.847705575887208	18.151313272044035
7	82.65637111095849	6.1577103835323435	7.247181640682489	3.938736864826679
8	82.60770596834803	5.569215372690613	7.3751238704486815	4.447954788512671
9	75.49749315646433	8.519539643449338	10.637650729486563	5.345316470599778
10	30.937294571285605	34.218070858802406	17.234524778995496	17.610109790916496
11	24.612395875236704	28.35568724796656	28.230688474406644	18.801228402390084
12	21.97937618351468	25.041748020525702	31.873117414468066	21.105758381491547
13	21.71171789915719	26.832546776424877	32.55207464604939	18.90366067836854
14	20.540614789886284	28.43574925677731	29.723020770989294	21.300615182347112
15	19.378734510061715	27.872960430137066	32.899991169631384	19.848313890169837
16	21.02471522061204	27.73167453223575	30.930230276390535	20.31337997076167
17	20.779034742594753	27.40946419285525	28.748148075470215	23.063352989079778
18	21.70171014805585	26.733254187066454	30.341342804721304	21.223692860156397
19	22.50959076147212	28.94359356756704	28.5266039383444	20.02021173261644
20	24.61121849275419	27.146319208014052	28.01699355383091	20.22546874540085
21	24.61435817937422	28.454194915669976	28.149449083113392	18.78199782184241
22	22.336123075715506	26.58019446434003	27.125322553742603	23.958359906201864
23	21.08554664887511	28.23637915640545	28.41534129374712	22.26273290097232
24	21.069259524533706	27.149851355461585	29.289351556597758	22.49153756340695
25	21.57926236987471	28.673188057417022	27.224418912687277	22.523130660020996
26	20.346150449858225	29.198104414203158	28.695362094170974	21.760383041767646
27	21.827297612857016	27.806242089461445	27.816446070976543	22.550014226705
28	20.06357865405559	28.798579291804437	29.594097389154346	21.543744664985624
29	22.259004523111038	27.320768045839422	28.28759529439468	22.13263213665486
30	23.059428380804743	26.66104139480578	27.665741113215137	22.61378911117434
31	21.879887363742505	28.272093091708285	26.784470325055683	23.063549219493527
32	22.895575985321965	27.886696559099693	27.798000412083866	21.419727043494472
33	21.54845419491567	28.31035802238989	27.158877954494166	22.982309828200272
34	21.05022517439978	26.63455028894928	29.43142237615408	22.883802160496856
35	22.701111645293903	25.987382384395758	27.50385102186988	23.807654948440458
36	22.21975844036067	28.6443421865955	26.82724855525358	22.308650817790248
37	21.307875707655928	28.143562170700836	28.409846842162068	22.138715279481165
38	21.556892102707	27.485601593390957	28.634334435494157	22.323171868407883
39	21.499592821891465	26.627093533226713	27.47049185153207	24.40282179334975
40	23.176970398642087	24.89732243600436	28.822126941454655	23.103580223898902
41	21.29610188283082	26.94341696019466	27.45283111429441	24.307650042680116
42	22.50507746195583	27.68222446797029	27.86864336103453	21.944054709039353
43	21.20917180953876	26.647893957084406	29.76442538829093	22.3785088450859
44	21.57592645284093	26.193816779662683	29.186134358964296	23.044122408532097
45	21.72152941984478	24.961686011714956	29.17612660786295	24.14065796057731
46	22.50743222692085	25.96501211722805	28.233435700199173	23.294119955651926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3498.0
1	2895.5
2	2293.0
3	3588.5
4	4884.0
5	3401.5
6	1919.0
7	1919.0
8	1800.5
9	1682.0
10	1742.5
11	1803.0
12	1733.0
13	1663.0
14	1668.5
15	1674.0
16	1724.5
17	1775.0
18	1729.0
19	1683.0
20	1683.0
21	1922.0
22	2161.0
23	2370.0
24	2579.0
25	3455.5
26	4332.0
27	5386.5
28	6441.0
29	7515.0
30	8589.0
31	8589.0
32	10013.5
33	11438.0
34	13364.5
35	15291.0
36	16534.0
37	17777.0
38	19468.0
39	21159.0
40	24548.5
41	27938.0
42	29752.5
43	31567.0
44	31567.0
45	36719.5
46	41872.0
47	45996.0
48	50120.0
49	50444.0
50	50768.0
51	47362.0
52	43956.0
53	38408.5
54	32861.0
55	29961.5
56	27062.0
57	27062.0
58	24957.0
59	22852.0
60	21327.0
61	19802.0
62	17524.0
63	15246.0
64	12921.0
65	10596.0
66	9084.5
67	7573.0
68	6578.0
69	5583.0
70	5583.0
71	4586.0
72	3589.0
73	2979.0
74	2369.0
75	1920.5
76	1472.0
77	1118.0
78	764.0
79	616.5
80	469.0
81	469.0
82	365.0
83	261.0
84	206.0
85	151.0
86	100.0
87	49.0
88	35.5
89	22.0
90	16.0
91	10.0
92	9.0
93	8.0
94	8.0
95	6.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	509605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.637070520316506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73551303767394	27.014531910129776
2	9.589956419463352	7.026958192532816
3	3.720386258976648	4.089121611988319
4	2.0893066221006094	3.061842962098572
5	1.4887278064784426	2.7271312815753403
6	1.0797457126865302	2.3735231891823485
7	0.8959957662389575	2.2978662051521193
8	0.7772496803031781	2.2780921079328795
9	0.6628376860126027	2.185598794137042
>10	5.8367320552280395	40.55510204710896
>50	0.08955950438017396	2.108466130232724
>100	0.029673329764514002	2.21065765449722
>500	0.0026975754331376367	0.7109920943514395
>1k	0.0016185452598825821	1.3601158190804157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3222	0.6322543931083878	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2566	0.5035272416871891	No Hit
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCT	1093	0.21447984223074734	No Hit
CTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTT	953	0.1870075843054915	No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCT	748	0.1467803494863669	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTCCGATCGT	731	0.1434444324525858	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	605	0.11871940031985558	No Hit
CCACCAGGGAGTAAAACTAATGACTTTTTTTTTTAATGAAATGTTT	560	0.10988903170102333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.962304137518274E-4	0.0	0.0	0.2048645519569078	0.0
2	1.962304137518274E-4	0.0	0.0	0.7515624846694989	0.0
3	1.962304137518274E-4	0.0	0.0	1.0612140775698826	0.0
4	1.962304137518274E-4	0.0	0.0	1.3822470344678721	0.0
5	1.962304137518274E-4	0.0	0.0	2.2382040992533434	0.0
6	1.962304137518274E-4	0.0	0.0	3.3123693841308466	0.0
7	1.962304137518274E-4	0.0	0.0	4.136733352302273	0.0
8	1.962304137518274E-4	0.0	0.0	5.755634265754849	0.0
9	1.962304137518274E-4	0.0	0.0	6.431059349888639	0.0
10	1.962304137518274E-4	0.0	0.0	7.703221122241736	0.0
11	1.962304137518274E-4	0.0	0.0	9.05230521678555	0.0
12	1.962304137518274E-4	0.0	0.0	10.145308621383228	0.0
13	1.962304137518274E-4	0.0	0.0	10.693380166992082	0.0
14	1.962304137518274E-4	0.0	0.0	10.928856663494274	0.0
15	1.962304137518274E-4	0.0	0.0	11.25695391528733	0.0
16	1.962304137518274E-4	0.0	0.0	11.967504243482697	0.0
17	1.962304137518274E-4	0.0	0.0	12.736138774148605	0.0
18	1.962304137518274E-4	0.0	0.0	13.741427183799217	0.0
19	1.962304137518274E-4	0.0	0.0	14.183730536395837	0.0
20	1.962304137518274E-4	0.0	0.0	14.66429881967406	0.0
21	1.962304137518274E-4	0.0	0.0	15.19353224556274	0.0
22	1.962304137518274E-4	0.0	0.0	15.751611542272936	0.0
23	1.962304137518274E-4	0.0	0.0	16.321660894221996	0.0
24	1.962304137518274E-4	0.0	0.0	16.757684873578555	0.0
25	3.924608275036548E-4	0.0	0.0	17.134447267982065	0.0
26	3.924608275036548E-4	0.0	0.0	17.4927640034929	0.0
27	3.924608275036548E-4	0.0	0.0	17.89640996458041	0.0
28	3.924608275036548E-4	0.0	0.0	18.280432884292736	0.0
29	3.924608275036548E-4	0.0	0.0	18.669165333935105	0.0
30	3.924608275036548E-4	0.0	0.0	19.200557294375056	0.0
31	3.924608275036548E-4	0.0	0.0	19.62323760559649	0.0
32	3.924608275036548E-4	0.0	0.0	20.019230580547678	0.0
33	3.924608275036548E-4	0.0	0.0	20.40462711315627	0.0
34	3.924608275036548E-4	0.0	0.0	20.782566890042286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	30	4.8621732E-6	40.000004	14
ACTAACG	30	4.8621732E-6	40.000004	1
TTGATCG	25	7.788541E-5	40.0	37
TCGTTGA	20	0.0012551112	40.0	24
AGTCACG	20	0.0012551112	40.0	1
GATCGCC	20	0.0012551112	40.0	9
AACCCGA	20	0.0012551112	40.0	24
TTAGATC	20	0.0012551112	40.0	13
TTTCGCG	25	7.788541E-5	40.0	1
CTATACG	25	7.788541E-5	40.0	1
TAAACGG	20	0.0012551112	40.0	2
TCTACGG	45	1.2187229E-9	40.0	2
TTAACGG	50	7.8216544E-11	40.0	2
CTATTAG	25	7.788541E-5	40.0	1
TAGATCG	20	0.0012551112	40.0	14
GACGCTT	20	0.0012551112	40.0	9
ATCGGGT	20	0.0012551112	40.0	4
TATACGT	20	0.0012551112	40.0	16
CGCTTAT	20	0.0012551112	40.0	11
AAGTCGT	25	7.788541E-5	40.0	25
>>END_MODULE