FastQCFastQC Report
Sat 18 Jun 2016
SRR3553830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790840
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44070.5572555763491983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33040.4177836224773659No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT14860.18790147185271355No Hit
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT10690.13517272773253755No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT10270.12986191897223207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG304.8655857E-640.01
CGTTGTA200.001255531240.036
CGTTGCG304.8655857E-640.017
TAGCGCG353.0547562E-740.01
TTGAGCG257.792445E-540.01
ATCCGAC257.792445E-540.027
ACGATAC200.001255531240.039
ATATACG353.0547562E-740.01
GTATCGT257.792445E-540.028
ACTCACG200.001255531240.01
TATCGTC257.792445E-540.029
TACAACG200.001255531240.01
CGTTTTT20200.036.7326741
ACGTAGG1000.036.02
GCTAACG503.421519E-936.01
CGATACT503.421519E-936.010
TAGGGCG3250.035.0769235
TGTATCG408.6953696E-735.027
CGTACGG408.6953696E-735.02
TCAACGG1100.034.5454522
Produced by FastQC (version 0.11.5)