##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553830_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	790840
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67294269384452	31.0	31.0	34.0	30.0	34.0
2	31.814403672044914	31.0	31.0	34.0	30.0	34.0
3	31.741122098022355	31.0	31.0	34.0	30.0	34.0
4	35.53676849931718	37.0	35.0	37.0	33.0	37.0
5	35.35203075211168	37.0	35.0	37.0	33.0	37.0
6	35.370976430124934	37.0	35.0	37.0	33.0	37.0
7	35.78670401092509	37.0	35.0	37.0	35.0	37.0
8	35.6409172525416	37.0	35.0	37.0	35.0	37.0
9	37.3723256284457	39.0	37.0	39.0	35.0	39.0
10	36.654596378534215	39.0	35.0	39.0	32.0	39.0
11	36.662041626624855	39.0	35.0	39.0	32.0	39.0
12	36.74970916999646	39.0	37.0	39.0	32.0	39.0
13	35.126254362450055	39.0	35.0	39.0	30.0	39.0
14	36.899446158515	39.0	36.0	41.0	30.0	41.0
15	37.499161650902835	39.0	36.0	41.0	32.0	41.0
16	37.68299656061909	39.0	36.0	41.0	32.0	41.0
17	37.730438521066205	39.0	37.0	41.0	33.0	41.0
18	37.73903065095342	39.0	37.0	41.0	33.0	41.0
19	37.726607151889134	39.0	37.0	41.0	33.0	41.0
20	37.68363765110515	39.0	36.0	41.0	33.0	41.0
21	37.56175332557787	39.0	36.0	41.0	32.0	41.0
22	37.533700875018965	39.0	36.0	41.0	32.0	41.0
23	37.469753679631786	39.0	36.0	41.0	32.0	41.0
24	37.44223989681857	39.0	36.0	41.0	32.0	41.0
25	37.25586085681048	39.0	35.0	41.0	32.0	41.0
26	37.246183804562236	39.0	36.0	41.0	32.0	41.0
27	37.227138232765164	39.0	35.0	41.0	32.0	41.0
28	37.064992918921654	39.0	35.0	40.0	31.0	41.0
29	36.98152976581862	39.0	35.0	40.0	31.0	41.0
30	36.826853725153	39.0	35.0	40.0	31.0	41.0
31	36.74795407414901	39.0	35.0	40.0	31.0	41.0
32	36.638156896464515	39.0	35.0	40.0	30.0	41.0
33	36.50664483334176	39.0	35.0	40.0	30.0	41.0
34	36.35746800869961	39.0	35.0	40.0	30.0	41.0
35	36.280936725507054	39.0	35.0	40.0	30.0	41.0
36	36.10971498659653	39.0	35.0	40.0	29.0	41.0
37	36.05937610641849	39.0	35.0	40.0	29.0	41.0
38	35.894725861109706	38.0	35.0	40.0	29.0	41.0
39	35.82553487431086	38.0	35.0	40.0	28.0	41.0
40	35.73005285519195	38.0	35.0	40.0	28.0	41.0
41	35.60006448839209	38.0	34.0	40.0	27.0	41.0
42	35.50071316574781	38.0	34.0	40.0	27.0	41.0
43	35.43352258358201	38.0	34.0	40.0	27.0	41.0
44	35.3237481665065	38.0	34.0	40.0	26.0	41.0
45	35.30914091345911	38.0	34.0	40.0	26.0	41.0
46	34.17299959536695	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	19.0
10	21.0
11	24.0
12	16.0
13	16.0
14	12.0
15	32.0
16	52.0
17	97.0
18	186.0
19	357.0
20	628.0
21	1047.0
22	1712.0
23	2451.0
24	3608.0
25	5041.0
26	7020.0
27	9500.0
28	11852.0
29	14104.0
30	17531.0
31	21479.0
32	27503.0
33	35663.0
34	50957.0
35	65322.0
36	81665.0
37	114495.0
38	164219.0
39	154207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.8727681958424	23.28726417480148	22.77679429467402	21.063173334682112
2	27.388473016033583	27.00407162004957	23.04789843710485	22.559556926811997
3	27.368873602751503	27.122426786707805	24.007384553133377	21.501315057407314
4	23.87044155581407	28.65080167922715	23.457336502958878	24.0214202619999
5	21.07000151737393	32.87163016539375	22.273658388548885	23.784709928683426
6	19.596504982044408	40.26781649891255	22.408704668453797	17.726973850589246
7	83.6841839057205	5.420439026857519	7.220297405290577	3.6750796621314046
8	83.41307976328966	4.891254868241363	7.329674776187344	4.365990592281625
9	76.03409033432806	7.778185220777907	10.617824085782205	5.569900359111831
10	32.045040716200496	29.09627737595468	19.912245207627333	18.94643670021749
11	26.085428152344342	28.876005260229633	25.097111931617018	19.941454655809014
12	22.069571594760003	24.446411410651965	32.100298416873194	21.383718577714834
13	22.35572302867837	26.129937787668805	32.645667897425525	18.868671286227304
14	21.437206008800768	27.270370745030597	30.46418997521623	20.828233270952403
15	20.67915128218097	26.81806686561125	32.96659248394113	19.536189368266655
16	21.95349248899904	26.324667442213347	30.418162965960242	21.303677102827372
17	22.11332254311871	26.527110414243083	28.74943098477568	22.610136057862526
18	22.755424611805168	25.81950837084619	29.76278387537302	21.66228314197562
19	24.176318850842144	28.03588589348035	27.51340346972839	20.274391785949117
20	25.036922765666887	26.234130797632897	27.331318597946485	21.39762783875373
21	25.66283951241718	27.68461382833443	27.446891912396943	19.20565474685145
22	23.33872844064539	26.00639826007789	27.502149613069648	23.15272368620707
23	21.99736988518537	27.397450811795053	27.485458499822972	23.1197208031966
24	21.55303221890648	26.702367103333163	28.880304486368924	22.864296191391432
25	21.980678771938695	27.764781751049515	27.368494259268626	22.88604521774316
26	21.64243083303829	27.56423549643417	28.34530372768196	22.448029942845583
27	22.914243083303827	26.47185271357038	27.982398462394414	22.631505740731374
28	22.02050983764099	27.754160133528906	28.651813261848165	21.573516766981943
29	23.495650194729656	27.023544585504023	26.952860249860905	22.527944969905416
30	24.35612766172677	26.90063729705124	26.716782155682566	22.026452885539427
31	24.074907693085834	27.782105103434322	26.152951292296798	21.990035911183046
32	24.41214404936523	27.488619695513634	26.815411461231097	21.28382479389004
33	24.31806686561125	27.010014667948006	26.558216579839154	22.11370188660159
34	23.749304536948053	27.895655252642758	27.063754994689194	21.291285215719995
35	24.361185574831826	26.107177178696073	27.807141773304334	21.72449547316777
36	24.440215467098277	28.65762986191897	26.64989125486824	20.25226341611451
37	24.800718223660915	27.819407212584085	27.42855697739113	19.951317586363867
38	23.00465328005665	27.99200849729402	28.398032471802136	20.6053057508472
39	25.029083000354053	25.953922411612968	27.292246219209954	21.724748368823025
40	24.681225026554042	25.191189115371	29.298341004501545	20.829244853573414
41	23.985761974609275	25.166531788983864	28.183071164837386	22.66463507156947
42	24.697283900662587	26.21756613221385	27.76263213797987	21.322517829143695
43	23.68114915785747	24.906302159728895	29.21526478175105	22.197283900662587
44	22.672980628192807	26.674548581255376	27.9136108441657	22.73885994638612
45	23.13691770775378	24.89555409438066	28.84237013808103	23.125158059784535
46	24.02951292296798	26.34806029032421	27.54602700925598	22.076399777451826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3571.0
1	3065.5
2	2560.0
3	5198.0
4	7836.0
5	5384.0
6	2932.0
7	2932.0
8	2805.5
9	2679.0
10	2655.5
11	2632.0
12	2642.0
13	2652.0
14	2486.0
15	2320.0
16	2418.0
17	2516.0
18	2430.0
19	2344.0
20	2344.0
21	2511.0
22	2678.0
23	3037.5
24	3397.0
25	4605.5
26	5814.0
27	6912.5
28	8011.0
29	9337.5
30	10664.0
31	10664.0
32	13202.5
33	15741.0
34	18078.0
35	20415.0
36	23120.5
37	25826.0
38	28104.5
39	30383.0
40	35466.0
41	40549.0
42	45022.0
43	49495.0
44	49495.0
45	56083.0
46	62671.0
47	70231.0
48	77791.0
49	78969.0
50	80147.0
51	75463.0
52	70779.0
53	63385.5
54	55992.0
55	50529.5
56	45067.0
57	45067.0
58	41449.0
59	37831.0
60	34378.5
61	30926.0
62	27821.5
63	24717.0
64	21765.0
65	18813.0
66	16118.0
67	13423.0
68	11986.0
69	10549.0
70	10549.0
71	8535.5
72	6522.0
73	5398.0
74	4274.0
75	3534.0
76	2794.0
77	2300.0
78	1806.0
79	1306.0
80	806.0
81	806.0
82	635.0
83	464.0
84	380.5
85	297.0
86	190.5
87	84.0
88	61.5
89	39.0
90	28.0
91	17.0
92	15.5
93	14.0
94	14.0
95	8.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	790840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.53458655810914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72549218180873	28.04790454395408
2	9.565102578822257	7.18044341363994
3	3.668576230726072	4.1309547623162866
4	1.9457173637612104	2.921267872308443
5	1.3838967288261732	2.5971995777805046
6	0.9995734704474777	2.2511146168620235
7	0.8218095008369503	2.1592395890408684
8	0.6895029386957643	2.0704166187637427
9	0.6024189617579828	2.0350391997916124
>10	5.456535651696909	40.112835362680315
>50	0.10921340494792942	2.5729896114577997
>100	0.028475874501721923	2.0238613750106755
>500	0.002010061729532857	0.47669825863091164
>1k	0.0016750514412773808	1.4200351977628292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4407	0.5572555763491983	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3304	0.4177836224773659	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	1486	0.18790147185271355	No Hit
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	1069	0.13517272773253755	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	1027	0.12986191897223207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.528956552526428E-4	0.0	0.0	0.1449092104597643	0.0
2	2.528956552526428E-4	0.0	0.0	0.6269283293713014	0.0
3	2.528956552526428E-4	0.0	0.0	0.8991705022507713	0.0
4	2.528956552526428E-4	0.0	0.0	1.1987254058975267	0.0
5	2.528956552526428E-4	0.0	0.0	1.9632289717262656	0.0
6	2.528956552526428E-4	0.0	0.0	2.9927671842597743	0.0
7	2.528956552526428E-4	0.0	0.0	3.8158160942795	0.0
8	2.528956552526428E-4	0.0	0.0	5.3904708917100805	0.0
9	2.528956552526428E-4	0.0	0.0	6.137145313843508	0.0
10	2.528956552526428E-4	0.0	0.0	7.44019017753275	0.0
11	2.528956552526428E-4	0.0	0.0	8.719589297455869	0.0
12	2.528956552526428E-4	0.0	0.0	9.802109149764807	0.0
13	2.528956552526428E-4	0.0	0.0	10.373906226291032	0.0
14	2.528956552526428E-4	0.0	0.0	10.646021951342876	0.0
15	2.528956552526428E-4	0.0	0.0	10.95594557685499	0.0
16	2.528956552526428E-4	0.0	0.0	11.664179859390016	0.0
17	2.528956552526428E-4	0.0	0.0	12.46080117343584	0.0
18	2.528956552526428E-4	0.0	0.0	13.482246725001264	0.0
19	2.528956552526428E-4	0.0	0.0	13.937711800111273	0.0
20	2.528956552526428E-4	0.0	0.0	14.470183602245713	0.0
21	2.528956552526428E-4	0.0	0.0	15.03161195690658	0.0
22	2.528956552526428E-4	0.0	0.0	15.621364624955744	0.0
23	2.528956552526428E-4	0.0	0.0	16.23160184108037	0.0
24	2.528956552526428E-4	0.0	0.0	16.70805725557635	0.0
25	2.528956552526428E-4	0.0	0.0	17.12571443022609	0.0
26	2.528956552526428E-4	0.0	0.0	17.51719690455718	0.0
27	2.528956552526428E-4	0.0	0.0	17.931313540033383	0.0
28	2.528956552526428E-4	0.0	0.0	18.3421425319913	0.0
29	2.528956552526428E-4	0.0	0.0	18.773708967679934	0.0
30	2.528956552526428E-4	0.0	0.0	19.30845683071165	0.0
31	2.528956552526428E-4	0.0	0.0	19.75861109706135	0.0
32	2.528956552526428E-4	0.0	0.0	20.21142076779121	0.0
33	2.528956552526428E-4	0.0	0.0	20.67156441252339	0.0
34	2.528956552526428E-4	0.0	0.0	21.124879874563756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	4.8655857E-6	40.0	1
CGTTGTA	20	0.0012555312	40.0	36
CGTTGCG	30	4.8655857E-6	40.0	17
TAGCGCG	35	3.0547562E-7	40.0	1
TTGAGCG	25	7.792445E-5	40.0	1
ATCCGAC	25	7.792445E-5	40.0	27
ACGATAC	20	0.0012555312	40.0	39
ATATACG	35	3.0547562E-7	40.0	1
GTATCGT	25	7.792445E-5	40.0	28
ACTCACG	20	0.0012555312	40.0	1
TATCGTC	25	7.792445E-5	40.0	29
TACAACG	20	0.0012555312	40.0	1
CGTTTTT	2020	0.0	36.732674	1
ACGTAGG	100	0.0	36.0	2
GCTAACG	50	3.421519E-9	36.0	1
CGATACT	50	3.421519E-9	36.0	10
TAGGGCG	325	0.0	35.076923	5
TGTATCG	40	8.6953696E-7	35.0	27
CGTACGG	40	8.6953696E-7	35.0	2
TCAACGG	110	0.0	34.545452	2
>>END_MODULE