FastQCFastQC Report
Sat 18 Jun 2016
SRR3553829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333621
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35581.066479628080966No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT11540.3459014870167046No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.3054364083795684No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT8830.26467158841919425No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8730.2616741751868138No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT8060.24159150652986475No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG7550.22630469904472442No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTA6970.20891970229691775No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.19033574025615893No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCG6060.1816432418822556No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGT5480.16425824513444898No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA5400.1618603145485446No Hit
ACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT4740.1420773872148336No Hit
GATCCGGGGAGTTGGGAGTTCCTATTTTAGGCAGAAGGGATTCTTT4110.12319368385083672No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT3700.11090428959807686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG257.782749E-540.01
ACAACGA353.0476804E-740.013
CGGGTCA200.001254488240.06
CAACGTG257.782749E-540.01
TTTACGG353.0476804E-740.02
ACGCACT257.782749E-540.011
GACGTAG353.0476804E-740.01
CATCTAG200.001254488240.039
GCCATAT257.782749E-540.036
TACGCTA257.782749E-540.035
TAACCGG200.001254488240.01
TGACACG200.001254488240.01
CGGGCTA200.001254488240.06
GGTATTC257.782749E-540.07
ACAGCCG200.001254488240.033
AACGAGC353.0476804E-740.015
CGTACTT200.001254488240.023
TTAACGG200.001254488240.02
GCGATAC555.456968E-1240.09
TAGATAG451.2150849E-940.01
Produced by FastQC (version 0.11.5)