##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553829_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333621
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.803927210817065	31.0	31.0	34.0	30.0	34.0
2	31.949139892272967	33.0	31.0	34.0	30.0	34.0
3	31.920148911489385	33.0	31.0	34.0	30.0	34.0
4	35.69032524930984	37.0	35.0	37.0	33.0	37.0
5	35.488440475869325	37.0	35.0	37.0	33.0	37.0
6	35.482373711486986	37.0	35.0	37.0	33.0	37.0
7	35.74904757194541	37.0	35.0	37.0	35.0	37.0
8	35.49039778671007	37.0	35.0	37.0	33.0	37.0
9	37.27875343578492	39.0	37.0	39.0	34.0	39.0
10	36.86075217087653	39.0	37.0	39.0	32.0	39.0
11	36.87106327239592	39.0	37.0	39.0	33.0	39.0
12	36.935135977651285	39.0	37.0	39.0	33.0	39.0
13	35.24291336576535	39.0	35.0	39.0	30.0	39.0
14	37.064819061150224	40.0	36.0	41.0	31.0	41.0
15	37.655828020418376	40.0	36.0	41.0	32.0	41.0
16	37.85607320882079	40.0	37.0	41.0	33.0	41.0
17	37.863473822091535	39.0	37.0	41.0	33.0	41.0
18	37.848049133597705	39.0	37.0	41.0	33.0	41.0
19	37.800857859667104	39.0	37.0	41.0	33.0	41.0
20	37.74316964459671	39.0	36.0	41.0	33.0	41.0
21	37.622349912025925	39.0	36.0	41.0	33.0	41.0
22	37.60898744383597	39.0	36.0	41.0	33.0	41.0
23	37.52800932794998	39.0	36.0	41.0	33.0	41.0
24	37.49434238252388	39.0	36.0	41.0	32.0	41.0
25	37.29880313289631	39.0	35.0	41.0	32.0	41.0
26	37.2841667640826	39.0	35.0	41.0	32.0	41.0
27	37.27160460522569	39.0	35.0	41.0	32.0	41.0
28	37.14953195392376	39.0	35.0	41.0	32.0	41.0
29	37.034521208197326	39.0	35.0	41.0	31.0	41.0
30	36.92916213307915	39.0	35.0	41.0	31.0	41.0
31	36.87466916051448	39.0	35.0	41.0	31.0	41.0
32	36.7496200778728	39.0	35.0	40.0	31.0	41.0
33	36.62698990770965	39.0	35.0	40.0	31.0	41.0
34	36.4899002161135	39.0	35.0	40.0	30.0	41.0
35	36.42776683721948	39.0	35.0	40.0	30.0	41.0
36	36.30000809301573	39.0	35.0	40.0	30.0	41.0
37	36.20090761672677	39.0	35.0	40.0	30.0	41.0
38	36.123340557099226	39.0	35.0	40.0	30.0	41.0
39	36.02721051732355	39.0	35.0	40.0	29.0	41.0
40	35.907107166515296	38.0	35.0	40.0	29.0	41.0
41	35.783721648217586	38.0	35.0	40.0	29.0	41.0
42	35.725760668543046	38.0	35.0	40.0	29.0	41.0
43	35.628440655714115	38.0	35.0	40.0	28.0	41.0
44	35.492460007013946	38.0	35.0	40.0	28.0	41.0
45	35.45752815320378	38.0	35.0	40.0	28.0	41.0
46	34.50118847434664	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	17.0
11	16.0
12	20.0
13	10.0
14	15.0
15	11.0
16	29.0
17	34.0
18	80.0
19	161.0
20	316.0
21	534.0
22	830.0
23	1168.0
24	1601.0
25	2114.0
26	2920.0
27	3407.0
28	4284.0
29	5107.0
30	6323.0
31	7860.0
32	10694.0
33	14360.0
34	21221.0
35	28207.0
36	34412.0
37	48431.0
38	71937.0
39	67491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.17224635139875	23.847719418142145	21.609850698846895	19.370183531612216
2	25.320948021857138	29.872819756550093	22.941601397993534	21.864630823599235
3	27.758144721105687	28.972696562866247	22.69041816912005	20.578740546908016
4	24.46278861342661	29.198102037941258	22.326532202709064	24.01257714592307
5	21.855638583902092	33.31894574981791	20.4651385854008	24.3602770808792
6	19.826389825580524	41.87446233900144	21.24866240434505	17.050485431072985
7	78.85744602408121	7.658690550055303	8.716777421085602	4.767086004777877
8	77.30748364161728	8.31722223720929	8.356488350553471	6.018805770619955
9	69.9767100991844	10.294316005287437	12.179389187131505	7.549584708396654
10	32.826470755737795	29.332386150751898	18.037533608495867	19.80360948501443
11	26.396419889635247	29.536509991877008	24.53502627232698	19.53204384616076
12	22.686521531917954	25.11472599146936	30.72318589057643	21.47556658603625
13	22.969777082377906	27.03756658004142	31.33256000071938	18.66009633686129
14	20.90815626114663	28.616304129536207	28.37111572712749	22.104423882189668
15	18.828251219197835	29.07011249291861	32.091205289834875	20.010430998048683
16	20.87458523294397	28.08456302211192	28.853699257540743	22.187152487403374
17	20.491815563168984	27.809700228702628	27.798310058419585	23.9001741497088
18	21.394036946115502	27.10920475629532	28.951115187593107	22.545643109996075
19	23.74400892030178	28.920241831299588	26.25494198506689	21.080807263331742
20	25.58472038630662	26.8664142844725	26.005856945456074	21.54300838376481
21	25.365609479019607	29.003569919159766	25.42166110646512	20.209159495355507
22	23.014138798217136	27.72547291687274	24.27185339052398	24.988534894386145
23	21.6059540616448	29.0629187011609	26.201588029530516	23.129539207663786
24	21.66710129158536	27.729969036721307	26.4000167855141	24.202912886179227
25	22.421250460852285	29.262846163760674	25.31855009127123	22.997353284115807
26	20.285293791457974	28.99247949019996	27.964666492816697	22.75756022552537
27	23.359740543910608	27.46619667227183	26.888894883715352	22.285167900102213
28	21.502843046450913	27.72337472761007	27.853762203218622	22.92002002272039
29	22.89693994083106	28.085462246081633	26.264233966087268	22.75336384700004
30	23.430179754871546	26.51571693628399	26.023241942203878	24.030861366640586
31	23.169704544977684	28.53957035078727	24.517641275579173	23.77308382865587
32	24.8416616459995	26.61013545310397	26.394921183019054	22.15328171787747
33	24.79939811942294	26.968326334373437	23.83123364536405	24.401041900839576
34	22.77764289418232	27.003695810515527	28.06837699065706	22.15028430464509
35	24.724163047290187	25.993867292526552	25.779552246411345	23.502417413771916
36	23.954127587891648	27.949979167978036	26.391923769786672	21.70396947434364
37	26.166518294711665	27.561814154384766	26.061608831578347	20.21005871932522
38	22.758759190818324	27.83877513705672	27.176946295347115	22.225519376777843
39	25.562239787063763	25.033196351548614	25.367108185635797	24.037455675751826
40	23.38641752167879	25.956099885798555	28.91664493542073	21.74083765710192
41	21.714160679333734	26.703055263307768	28.140914390880674	23.441869666477828
42	23.65018988612827	26.200089322914327	28.800944784650845	21.348776006306558
43	25.031397903609186	25.107232458388413	28.22244403080142	21.638925607200985
44	23.546479388287906	25.8083274134422	27.181442415195683	23.463750783074207
45	24.13037548595562	24.09500600981353	27.754248083903594	24.020370420327257
46	23.542283009762574	25.580823749104525	27.10920475629532	23.767688484837585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3847.0
1	3296.0
2	2745.0
3	2691.5
4	2638.0
5	1969.5
6	1301.0
7	1301.0
8	1231.5
9	1162.0
10	1188.5
11	1215.0
12	1249.0
13	1283.0
14	1223.0
15	1163.0
16	1189.0
17	1215.0
18	1149.0
19	1083.0
20	1083.0
21	1017.5
22	952.0
23	1212.0
24	1472.0
25	2306.5
26	3141.0
27	3097.5
28	3054.0
29	4430.5
30	5807.0
31	5807.0
32	5948.5
33	6090.0
34	6509.5
35	6929.0
36	8322.0
37	9715.0
38	10153.5
39	10592.0
40	12761.0
41	14930.0
42	17248.5
43	19567.0
44	19567.0
45	22593.0
46	25619.0
47	28909.5
48	32200.0
49	32399.5
50	32599.0
51	29696.5
52	26794.0
53	24195.0
54	21596.0
55	19667.0
56	17738.0
57	17738.0
58	17466.0
59	17194.0
60	15958.5
61	14723.0
62	13434.0
63	12145.0
64	10627.5
65	9110.0
66	8377.5
67	7645.0
68	6706.5
69	5768.0
70	5768.0
71	4990.0
72	4212.0
73	3393.0
74	2574.0
75	2136.0
76	1698.0
77	1470.5
78	1243.0
79	867.5
80	492.0
81	492.0
82	368.5
83	245.0
84	152.5
85	60.0
86	54.0
87	48.0
88	30.0
89	12.0
90	8.0
91	4.0
92	2.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	333621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.907980883310483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22546820822399	22.632513715512466
2	10.171151052993809	6.2873948461439175
3	3.782107740078892	3.50691941186937
4	2.009766536530025	2.484713027639485
5	1.27542490924437	1.9710404356511497
6	0.9248454531955979	1.7151063352431697
7	0.7161049959632881	1.5493351667973505
8	0.6112359671425729	1.5113655670107549
9	0.5303370800696044	1.4752483499251832
>10	6.343071164928629	43.88640028825467
>50	0.35655430732757704	6.877701018612569
>100	0.04194756556795024	2.3750911865890982
>500	0.008988764050275051	1.9580456715017742
>1k	0.0029962546834250174	1.7691249792490409
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3558	1.066479628080966	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	1154	0.3459014870167046	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.3054364083795684	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	883	0.26467158841919425	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	873	0.2616741751868138	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	806	0.24159150652986475	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	755	0.22630469904472442	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTA	697	0.20891970229691775	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	635	0.19033574025615893	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCG	606	0.1816432418822556	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGT	548	0.16425824513444898	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	540	0.1618603145485446	No Hit
ACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	474	0.1420773872148336	No Hit
GATCCGGGGAGTTGGGAGTTCCTATTTTAGGCAGAAGGGATTCTTT	411	0.12319368385083672	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	370	0.11090428959807686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26467158841919425	0.0
2	0.0	0.0	0.0	1.177683659002281	0.0
3	0.0	0.0	0.0	1.5151923889683203	0.0
4	0.0	0.0	0.0	1.9504167903099625	0.0
5	0.0	0.0	0.0	3.164069408100809	0.0
6	0.0	0.0	0.0	4.331861603436234	0.0
7	0.0	0.0	0.0	5.228987383887705	0.0
8	0.0	0.0	0.0	7.0277350646392165	0.0
9	0.0	0.0	0.0	7.807961729027849	0.0
10	0.0	0.0	0.0	9.400187638068347	0.0
11	0.0	0.0	0.0	10.864723743409437	0.0
12	0.0	0.0	0.0	12.16500160361608	0.0
13	0.0	0.0	0.0	12.650582547261713	0.0
14	0.0	0.0	0.0	12.861000956174822	0.0
15	0.0	0.0	0.0	13.191016153059909	0.0
16	0.0	0.0	0.0	13.890012918851031	0.0
17	0.0	0.0	0.0	14.669640100593188	0.0
18	0.0	0.0	0.0	15.804760491695667	0.0
19	0.0	0.0	0.0	16.244780754209117	0.0
20	0.0	0.0	0.0	16.674909553055713	0.0
21	0.0	0.0	0.0	17.13081610570078	0.0
22	0.0	0.0	0.0	17.60950299891194	0.0
23	0.0	0.0	0.0	18.127156264144045	0.0
24	0.0	0.0	0.0	18.497936280989506	0.0
25	0.0	0.0	0.0	18.8240548406725	0.0
26	0.0	0.0	0.0	19.11989952670845	0.0
27	0.0	0.0	0.0	19.507165316332006	0.0
28	0.0	0.0	0.0	19.845873011590996	0.0
29	0.0	0.0	0.0	20.200766738304843	0.0
30	0.0	0.0	0.0	20.691743025768762	0.0
31	0.0	0.0	0.0	21.040641926017848	0.0
32	0.0	0.0	0.0	21.38834186097398	0.0
33	0.0	0.0	0.0	21.71356119668726	0.0
34	0.0	0.0	0.0	22.0171991571274	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	7.782749E-5	40.0	1
ACAACGA	35	3.0476804E-7	40.0	13
CGGGTCA	20	0.0012544882	40.0	6
CAACGTG	25	7.782749E-5	40.0	1
TTTACGG	35	3.0476804E-7	40.0	2
ACGCACT	25	7.782749E-5	40.0	11
GACGTAG	35	3.0476804E-7	40.0	1
CATCTAG	20	0.0012544882	40.0	39
GCCATAT	25	7.782749E-5	40.0	36
TACGCTA	25	7.782749E-5	40.0	35
TAACCGG	20	0.0012544882	40.0	1
TGACACG	20	0.0012544882	40.0	1
CGGGCTA	20	0.0012544882	40.0	6
GGTATTC	25	7.782749E-5	40.0	7
ACAGCCG	20	0.0012544882	40.0	33
AACGAGC	35	3.0476804E-7	40.0	15
CGTACTT	20	0.0012544882	40.0	23
TTAACGG	20	0.0012544882	40.0	2
GCGATAC	55	5.456968E-12	40.0	9
TAGATAG	45	1.2150849E-9	40.0	1
>>END_MODULE