FastQCFastQC Report
Sat 18 Jun 2016
SRR3553828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408061
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35690.8746241370775448No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.30706193436765583No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT8730.21393860231681047No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8630.21148798831547244No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT7600.1862466641016907No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7580.18575654130142308No Hit
AGTTGAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGT7340.1798750676982118No Hit
AGTTGAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACT6290.1541436206841624No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGT5360.13135291047171868No Hit
AGTTGAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGA5020.12302082286716938No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT4880.11958996326529611No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA4660.11419861246235244No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAG200.001254817240.01
ACGCGAG200.001254817240.01
GTCATAC200.001254817240.09
TCGTTGA257.7858094E-540.024
CTGTCGC200.001254817240.026
ACGCCCG200.001254817240.020
CGTTGAT257.7858094E-540.025
TTATGAG200.001254817240.01
GTTAGCG257.7858094E-540.01
ATATGCG200.001254817240.01
TACGGGT257.7858094E-540.04
TCGATGG257.7858094E-540.02
TCGATAG200.001254817240.01
AACGGAC200.001254817240.04
TCGGACA200.001254817240.021
CCGTAAG200.001254817240.01
TGCGCGT200.001254817240.037
ACGGACT200.001254817240.05
GGTATTA304.8597885E-640.08
AATCTAG200.001254817240.01
Produced by FastQC (version 0.11.5)