##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553828_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408061
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79170271111427	31.0	31.0	34.0	30.0	34.0
2	31.95465138790524	33.0	31.0	34.0	30.0	34.0
3	31.924967100507033	33.0	31.0	34.0	30.0	34.0
4	35.678383869078395	37.0	35.0	37.0	33.0	37.0
5	35.457492874839794	37.0	35.0	37.0	33.0	37.0
6	35.49735211157155	37.0	35.0	37.0	33.0	37.0
7	35.69374431763878	37.0	35.0	37.0	35.0	37.0
8	35.51438878010886	37.0	35.0	37.0	33.0	37.0
9	37.326218384016116	39.0	37.0	39.0	34.0	39.0
10	36.83556870173822	39.0	37.0	39.0	32.0	39.0
11	36.814388044924655	39.0	37.0	39.0	32.0	39.0
12	36.873947767613174	39.0	37.0	39.0	33.0	39.0
13	35.1796765679641	39.0	35.0	39.0	30.0	39.0
14	37.00341370530386	40.0	36.0	41.0	30.0	41.0
15	37.626178439007894	40.0	36.0	41.0	32.0	41.0
16	37.83142226284796	40.0	37.0	41.0	33.0	41.0
17	37.86992876065098	40.0	37.0	41.0	33.0	41.0
18	37.87473686532161	39.0	37.0	41.0	33.0	41.0
19	37.83232899002845	40.0	37.0	41.0	33.0	41.0
20	37.79653532192491	40.0	36.0	41.0	33.0	41.0
21	37.64595489399869	39.0	36.0	41.0	33.0	41.0
22	37.62309311598021	39.0	36.0	41.0	33.0	41.0
23	37.530837301285835	39.0	36.0	41.0	32.0	41.0
24	37.38805717772588	39.0	36.0	41.0	32.0	41.0
25	37.19458365293424	39.0	35.0	41.0	32.0	41.0
26	37.179558938492036	39.0	35.0	41.0	32.0	41.0
27	37.12981392487888	39.0	35.0	41.0	32.0	41.0
28	37.00863351312671	39.0	35.0	41.0	31.0	41.0
29	36.94445928427368	39.0	35.0	41.0	31.0	41.0
30	36.81790712663058	39.0	35.0	41.0	31.0	41.0
31	36.73946052183375	39.0	35.0	41.0	31.0	41.0
32	36.57404652735743	39.0	35.0	40.0	30.0	41.0
33	36.45730907878969	39.0	35.0	40.0	30.0	41.0
34	36.348065607838045	39.0	35.0	40.0	30.0	41.0
35	36.252366680471795	39.0	35.0	40.0	30.0	41.0
36	36.11395355106222	39.0	35.0	40.0	29.0	41.0
37	36.03063757624473	39.0	35.0	40.0	29.0	41.0
38	35.91667912395451	38.0	35.0	40.0	29.0	41.0
39	35.80610251898613	38.0	35.0	40.0	28.0	41.0
40	35.65163786786779	38.0	35.0	40.0	28.0	41.0
41	35.511119661031074	38.0	35.0	40.0	27.0	41.0
42	35.441806494617225	38.0	34.0	40.0	27.0	41.0
43	35.3637274819206	38.0	34.0	40.0	27.0	41.0
44	35.226017678729406	38.0	34.0	40.0	26.0	41.0
45	35.153080054207585	38.0	34.0	40.0	26.0	41.0
46	33.97008290427166	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	17.0
11	14.0
12	8.0
13	9.0
14	8.0
15	17.0
16	31.0
17	68.0
18	147.0
19	227.0
20	466.0
21	755.0
22	1138.0
23	1682.0
24	2333.0
25	3044.0
26	3809.0
27	4573.0
28	5524.0
29	6805.0
30	8264.0
31	10542.0
32	13353.0
33	17916.0
34	25914.0
35	33850.0
36	41255.0
37	58090.0
38	82881.0
39	85312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.96452981294463	24.957298051026687	21.10983406892597	19.96833806710271
2	26.34851161958629	28.305081838254576	23.07057033139653	22.275836210762606
3	28.167112269979246	27.013853320949565	24.040033230325857	20.779001178745336
4	24.60833061723615	28.489122949755064	23.454091422605934	23.448455010402856
5	22.592210478335346	32.94973055499055	20.03695525914018	24.421103707533923
6	19.350783338765527	42.57304667684488	20.51972621740377	17.55644376698582
7	79.99931382807962	9.10721681317254	6.8842648525588075	4.009204506189025
8	79.55232183423557	7.232497102148944	8.398989366785848	4.816191696829641
9	74.43666510644242	9.456674369763345	9.787752321344113	6.318908202450125
10	32.78848015370251	32.20082291618165	16.580364210252878	18.430332719862964
11	26.435508416633784	29.00007596903404	25.01954364666067	19.5448719676715
12	23.06395367359292	27.105996407399875	29.40075135825281	20.429298560754397
13	22.945834078728424	27.841180607801284	28.97164884661852	20.24133646685177
14	22.322642938188164	29.423787129865385	26.918769497697646	21.334800434248802
15	20.29304442228	27.915924334842096	29.863182220305294	21.927849022572605
16	21.436255853904196	27.043995873166022	28.816770041733957	22.702978231195825
17	22.111645072672957	26.917789252097112	27.018999610352374	23.951566064877554
18	23.11541656762102	27.663266031304147	26.694293255175083	22.527024145899755
19	24.215007070021393	27.837749748199414	26.19608342870306	21.75115975307613
20	25.691256944427426	26.83250788485055	25.67042672541605	21.80580844530597
21	26.172557534290213	28.396734801904618	25.71870382124241	19.71200384256275
22	23.63029056930214	27.00258049654341	24.57304177561688	24.79408715853757
23	22.46673904146684	29.018700635444212	25.067575681086897	23.446984642002054
24	22.331710209993112	28.747417665496087	26.990082365136587	21.93078975937421
25	22.471640269469514	29.78133171266061	24.403949409524557	23.343078608345323
26	21.45144966071249	29.228228132558616	26.22867659492086	23.09164561180804
27	22.75150038842232	27.941165659055876	26.199269226904804	23.108064725617
28	21.931770004974744	28.671203570054477	27.352283114534348	22.04474331043643
29	21.916576198166453	29.238765772764364	25.871622134925904	22.97303589414328
30	23.370525485160307	27.90391632623554	25.90151962574223	22.824038562861926
31	24.248825543239857	29.828628562886433	23.848395215421224	22.074150678452487
32	22.815706475257375	30.32340752975658	24.207900289417513	22.65298570556853
33	23.62980044650187	28.229847988413496	23.901083416449993	24.23926814863464
34	21.96313786419187	29.794074905467564	25.298178458612806	22.944608771727758
35	23.930735845866185	28.1783850943854	24.17677749160052	23.7141015681479
36	23.013225963765223	30.43687095801853	23.696947270138534	22.852955808077713
37	23.600638139885948	29.626207846375905	25.022974506262546	21.750179507475597
38	22.669894942177763	29.927878429940623	25.411641886874758	21.99058474100686
39	22.845849027473832	26.39874920661372	25.066350374086227	25.689051391826222
40	24.133891746577106	26.830302332249346	27.238574624872264	21.79723129630129
41	22.24446835154548	27.690467846718995	26.148051394276834	23.91701240745869
42	22.625048705953276	28.58641232560818	25.444480114492684	23.344058853945857
43	22.649309784566523	26.37546837360101	27.397619473559097	23.57760236827337
44	22.513055646092127	27.34983250053301	26.4943231526659	23.642788700708962
45	23.88098838163902	25.566275630359186	27.14226549461968	23.410470493382114
46	23.1058591730158	26.618569282533738	27.419920060971275	22.855651483479186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3884.0
1	3442.0
2	3000.0
3	3047.0
4	3094.0
5	2265.0
6	1436.0
7	1436.0
8	1397.5
9	1359.0
10	1384.5
11	1410.0
12	1437.0
13	1464.0
14	1414.5
15	1365.0
16	1384.5
17	1404.0
18	1523.5
19	1643.0
20	1643.0
21	1594.0
22	1545.0
23	2265.5
24	2986.0
25	3145.5
26	3305.0
27	3616.0
28	3927.0
29	5351.0
30	6775.0
31	6775.0
32	7177.5
33	7580.0
34	8413.5
35	9247.0
36	10505.0
37	11763.0
38	13179.0
39	14595.0
40	17686.0
41	20777.0
42	22681.5
43	24586.0
44	24586.0
45	27087.0
46	29588.0
47	32867.0
48	36146.0
49	35913.5
50	35681.0
51	33579.5
52	31478.0
53	28966.5
54	26455.0
55	24683.0
56	22911.0
57	22911.0
58	21763.0
59	20615.0
60	19833.5
61	19052.0
62	17308.0
63	15564.0
64	14290.5
65	13017.0
66	11463.5
67	9910.0
68	8505.0
69	7100.0
70	7100.0
71	6245.5
72	5391.0
73	4381.0
74	3371.0
75	2675.5
76	1980.0
77	1749.0
78	1518.0
79	1046.5
80	575.0
81	575.0
82	463.5
83	352.0
84	241.0
85	130.0
86	87.5
87	45.0
88	30.0
89	15.0
90	14.5
91	14.0
92	10.0
93	6.0
94	6.0
95	3.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	408061.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.11069727029277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91090081990859	23.616410549233954
2	8.952990778361764	5.570675715386714
3	3.2649369702907243	3.047233970679048
4	1.7503464297899598	2.1781799158133284
5	1.1095256358753407	1.7259058085673407
6	0.8081996823468116	1.508619339086306
7	0.6271144274292045	1.3656976973908077
8	0.5101848696377519	1.2697765624947104
9	0.4959596491731413	1.3886685453335585
>10	6.032818140040964	43.34743450285506
>50	0.4746332071832182	9.297036167625668
>100	0.054491998828023386	3.110232051954898
>500	0.006317912907596915	1.389395864902832
>1k	0.0015794782268992288	1.1847333086757657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3569	0.8746241370775448	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1253	0.30706193436765583	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	873	0.21393860231681047	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	863	0.21148798831547244	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	760	0.1862466641016907	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	758	0.18575654130142308	No Hit
AGTTGAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGT	734	0.1798750676982118	No Hit
AGTTGAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACT	629	0.1541436206841624	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGT	536	0.13135291047171868	No Hit
AGTTGAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGA	502	0.12302082286716938	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	488	0.11958996326529611	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	466	0.11419861246235244	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.450614001338035E-4	0.0	0.0	0.24530646153393731	0.0
2	2.450614001338035E-4	0.0	0.0	0.7467020862076993	0.0
3	2.450614001338035E-4	0.0	0.0	0.9611308113247774	0.0
4	2.450614001338035E-4	0.0	0.0	1.2799556928988558	0.0
5	2.450614001338035E-4	0.0	0.0	1.898490666836576	0.0
6	2.450614001338035E-4	0.0	0.0	2.6483785512460147	0.0
7	2.450614001338035E-4	0.0	0.0	3.19315004374346	0.0
8	2.450614001338035E-4	0.0	0.0	4.287104133940759	0.0
9	2.450614001338035E-4	0.0	0.0	4.881378029265233	0.0
10	2.450614001338035E-4	0.0	0.0	5.8782878050095455	0.0
11	2.450614001338035E-4	0.0	0.0	7.192797155327267	0.0
12	2.450614001338035E-4	0.0	0.0	8.127461335437594	0.0
13	2.450614001338035E-4	0.0	0.0	8.529852154457298	0.0
14	2.450614001338035E-4	0.0	0.0	8.89719919325787	0.0
15	2.450614001338035E-4	0.0	0.0	9.158924768600773	0.0
16	2.450614001338035E-4	0.0	0.0	9.90023550400553	0.0
17	2.450614001338035E-4	0.0	0.0	10.536169837352748	0.0
18	2.450614001338035E-4	0.0	0.0	11.4318692548418	0.0
19	2.450614001338035E-4	0.0	0.0	11.823477372255619	0.0
20	2.450614001338035E-4	0.0	0.0	12.222192270273316	0.0
21	2.450614001338035E-4	0.0	0.0	12.60252756328098	0.0
22	2.450614001338035E-4	0.0	0.0	12.996341233296002	0.0
23	2.450614001338035E-4	0.0	0.0	13.514646094578996	0.0
24	2.450614001338035E-4	0.0	0.0	13.84278330935816	0.0
25	2.450614001338035E-4	0.0	0.0	14.113086033705745	0.0
26	2.450614001338035E-4	0.0	0.0	14.41500167867059	0.0
27	2.450614001338035E-4	0.0	0.0	14.738237665447079	0.0
28	2.450614001338035E-4	0.0	0.0	15.033046529808043	0.0
29	2.450614001338035E-4	0.0	0.0	15.334472051972622	0.0
30	2.450614001338035E-4	0.0	0.0	15.700838845172658	0.0
31	2.450614001338035E-4	0.0	0.0	15.988785990329877	0.0
32	2.450614001338035E-4	0.0	0.0	16.277468319687497	0.0
33	2.450614001338035E-4	0.0	0.0	16.561004359642308	0.0
34	2.450614001338035E-4	0.0	0.0	16.832777452390697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAG	20	0.0012548172	40.0	1
ACGCGAG	20	0.0012548172	40.0	1
GTCATAC	20	0.0012548172	40.0	9
TCGTTGA	25	7.7858094E-5	40.0	24
CTGTCGC	20	0.0012548172	40.0	26
ACGCCCG	20	0.0012548172	40.0	20
CGTTGAT	25	7.7858094E-5	40.0	25
TTATGAG	20	0.0012548172	40.0	1
GTTAGCG	25	7.7858094E-5	40.0	1
ATATGCG	20	0.0012548172	40.0	1
TACGGGT	25	7.7858094E-5	40.0	4
TCGATGG	25	7.7858094E-5	40.0	2
TCGATAG	20	0.0012548172	40.0	1
AACGGAC	20	0.0012548172	40.0	4
TCGGACA	20	0.0012548172	40.0	21
CCGTAAG	20	0.0012548172	40.0	1
TGCGCGT	20	0.0012548172	40.0	37
ACGGACT	20	0.0012548172	40.0	5
GGTATTA	30	4.8597885E-6	40.0	8
AATCTAG	20	0.0012548172	40.0	1
>>END_MODULE