FastQCFastQC Report
Sat 18 Jun 2016
SRR3553825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472254
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45990.9738403486259514No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT20160.42688891994562245No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT13770.2915803783557153No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT12680.2684995786165919No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11730.24838328526597975No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.22932574419697876No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8900.1884579061267877No Hit
AAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG7210.15267207900833027No Hit
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT6410.13573204250255158No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGT6350.1344615397646182No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA6330.1340380388519737No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT6200.1312852829197847No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4930.10439297496686105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA304.8614147E-640.00000431
GGCGAAT304.8614147E-640.0000048
CTTAACG200.001255017740.01
AGACTAT200.001255017740.025
TCGTTGA200.001255017740.024
GGTACAA257.787672E-540.08
GGTCGAT200.001255017740.08
TTATGAA200.001255017740.014
TAAACGG401.924127E-840.02
TGAACGG555.456968E-1240.02
GTGATAG257.787672E-540.01
ATAGACG257.787672E-540.01
TTCCGCA200.001255017740.01
GCTTGCG200.001255017740.01
ATCGTTG200.001255017740.023
TATTCGG200.001255017740.02
GTCGTGT200.001255017740.032
GATATAC200.001255017740.09
TGCCTAA200.001255017740.023
TTAACGG507.8216544E-1140.02
Produced by FastQC (version 0.11.5)