##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472254
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.618590843063267	31.0	31.0	34.0	30.0	34.0
2	31.772281441766506	31.0	31.0	34.0	30.0	34.0
3	31.76366955070788	31.0	31.0	34.0	30.0	34.0
4	35.53954651522274	37.0	35.0	37.0	33.0	37.0
5	35.32991568096829	37.0	35.0	37.0	33.0	37.0
6	35.328037454420716	37.0	35.0	37.0	32.0	37.0
7	35.66509124327163	37.0	35.0	37.0	35.0	37.0
8	35.446389019468334	37.0	35.0	37.0	33.0	37.0
9	37.199797143062845	39.0	37.0	39.0	34.0	39.0
10	36.65976360179056	39.0	35.0	39.0	32.0	39.0
11	36.650952665302995	39.0	35.0	39.0	32.0	39.0
12	36.74901430162582	39.0	35.0	39.0	32.0	39.0
13	35.105362792056816	39.0	35.0	39.0	30.0	39.0
14	36.83049375971405	39.0	36.0	41.0	30.0	41.0
15	37.4343404185036	39.0	36.0	41.0	32.0	41.0
16	37.64519305289103	39.0	36.0	41.0	32.0	41.0
17	37.67158986477616	39.0	36.0	41.0	33.0	41.0
18	37.70382887175122	39.0	37.0	41.0	33.0	41.0
19	37.65627607177493	39.0	36.0	41.0	33.0	41.0
20	37.61294557589772	39.0	36.0	41.0	33.0	41.0
21	37.4561676555413	39.0	36.0	41.0	32.0	41.0
22	37.466905521181395	39.0	36.0	41.0	32.0	41.0
23	37.366165241586096	39.0	36.0	41.0	32.0	41.0
24	37.33935551631114	39.0	36.0	41.0	32.0	41.0
25	37.14657366586625	39.0	35.0	40.0	31.0	41.0
26	37.16132632015822	39.0	35.0	40.0	32.0	41.0
27	37.128756982471295	39.0	35.0	40.0	32.0	41.0
28	36.983189131272574	39.0	35.0	40.0	31.0	41.0
29	36.91545227779966	39.0	35.0	40.0	31.0	41.0
30	36.778392559935966	39.0	35.0	40.0	31.0	41.0
31	36.68206304234586	39.0	35.0	40.0	31.0	41.0
32	36.566792446437724	39.0	35.0	40.0	30.0	41.0
33	36.50595865784091	39.0	35.0	40.0	30.0	41.0
34	36.340609079012566	39.0	35.0	40.0	30.0	41.0
35	36.284207227466574	39.0	35.0	40.0	30.0	41.0
36	36.13937838536042	38.0	35.0	40.0	30.0	41.0
37	36.055808950268286	38.0	35.0	40.0	30.0	41.0
38	35.92628331364054	38.0	35.0	40.0	29.0	41.0
39	35.86000330330712	38.0	35.0	40.0	29.0	41.0
40	35.70902946295849	38.0	35.0	40.0	28.0	41.0
41	35.59646715538672	38.0	34.0	40.0	28.0	41.0
42	35.567362055165226	38.0	34.0	40.0	28.0	41.0
43	35.53590652487856	38.0	34.0	40.0	28.0	41.0
44	35.410867880420284	38.0	34.0	40.0	27.0	41.0
45	35.31885807213914	38.0	34.0	40.0	27.0	41.0
46	34.19794856157915	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	25.0
11	16.0
12	12.0
13	8.0
14	11.0
15	15.0
16	27.0
17	61.0
18	108.0
19	224.0
20	386.0
21	677.0
22	1037.0
23	1565.0
24	2191.0
25	2961.0
26	3802.0
27	4813.0
28	6279.0
29	7743.0
30	9973.0
31	12864.0
32	17015.0
33	22525.0
34	32318.0
35	42427.0
36	52343.0
37	70750.0
38	97332.0
39	82735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.433817394876485	25.17352949895607	21.522739881504442	20.869913224663
2	24.640130099480366	28.821989861388154	22.93977393521283	23.598106103918653
3	27.2567304882542	28.32712904496309	23.255282115132957	21.160858351649747
4	25.003493882529316	29.794771457732494	21.16107010210607	24.040664557632123
5	20.10591757825238	33.055940235551205	20.204169789985897	26.633972396210513
6	18.893434465351273	41.45904534424272	21.19029166507854	18.45722852532747
7	79.69461349189207	7.359598859935542	7.923066824209006	5.022720823963376
8	78.06117047182238	6.769873839078124	8.288548111821182	6.880407577278329
9	70.65583351332123	9.156513232286017	12.891367780897568	7.296285473495195
10	31.93641557297556	28.395736192811494	19.06770509090447	20.600143143308475
11	24.813765473664596	29.26179555916943	25.226467113036627	20.697971854129346
12	21.66630669089092	24.175761348765707	31.737793644945306	22.42013831539807
13	21.574195242390747	26.312535203513367	32.38278553490283	19.730484019193064
14	19.572306428320353	28.49695291093352	29.033740317710382	22.89700034303574
15	17.86051573941142	27.855137277820834	32.776853134118504	21.507493848649244
16	19.070246096380338	26.66425271146459	30.19074481105507	24.074756381100002
17	18.448758507074583	27.17414781028853	28.033219411587833	26.343874271049057
18	20.354089113062038	26.92978778369267	28.373925895810302	24.34219720743498
19	22.00680142465707	29.561422454865387	26.734342112507253	21.697434007970287
20	23.81578557301791	26.834711828803993	25.55023356075332	23.799269037424775
21	23.838654622300712	29.15380282644509	26.530849923981588	20.476692627272612
22	21.240264772770583	26.599457071829992	24.548230401436516	27.61204775396291
23	19.664417876820526	29.36047127181559	25.558703579006213	25.41640727235767
24	20.997598749825304	26.914541750837472	27.05747330885498	25.03038619048224
25	21.140530307842813	28.737924930228225	24.89296014432911	25.228584617599854
26	19.04293028751477	28.022420138315397	27.53539408877426	25.39925548539557
27	21.817920017617638	26.799773003510825	25.87442350938266	25.507883469488878
28	19.612115514108933	28.94332287286079	27.333384153442854	24.111177459587427
29	20.54000601371296	28.030043154743	25.0147166567144	26.41523417482965
30	21.76858216129456	25.83884943272053	25.424453789697914	26.968114616286996
31	21.072346660907055	29.606101801149382	22.627442012137536	26.694109525806027
32	19.402270811893597	29.961842567770734	24.133834758413904	26.50205186192176
33	19.234140949573746	26.69707403219454	22.188695066637866	31.880089951593842
34	18.12499205935789	29.087313183159907	25.18094076492735	27.606753992554857
35	19.423445857525824	26.744929635323366	23.551944504440407	30.279680002710407
36	19.43911539129367	29.1243695130163	23.040567152422213	28.395947943267817
37	19.091844642925203	29.77084365616808	23.687253046030314	27.450058654876404
38	18.865271654660415	30.401013014183047	23.404142685927486	27.32957264522905
39	20.467375607194434	25.157859965188223	23.622245655939388	30.75251877167795
40	21.009456775379352	26.009096799603604	28.001245092683174	24.98020133233387
41	18.073324948015262	27.34079541941413	26.429209704946917	28.156669927623696
42	20.372511402762072	27.503843270782248	26.337945258272033	25.785700068183647
43	20.36869989454827	23.93055432034456	28.42199324939545	27.278752535711714
44	20.718088147479957	25.790358578222737	26.170238896864824	27.321314377432483
45	21.277532853083297	23.283233175367492	26.945033816547877	28.49420015500133
46	22.616854489321426	25.600418418901693	25.861294981090683	25.921432110686197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4968.0
1	4216.0
2	3464.0
3	3132.5
4	2801.0
5	2027.0
6	1253.0
7	1253.0
8	1202.0
9	1151.0
10	1210.5
11	1270.0
12	1311.0
13	1352.0
14	1372.5
15	1393.0
16	1326.0
17	1259.0
18	1376.0
19	1493.0
20	1493.0
21	1499.0
22	1505.0
23	1803.0
24	2101.0
25	2436.5
26	2772.0
27	3174.0
28	3576.0
29	4206.0
30	4836.0
31	4836.0
32	6034.0
33	7232.0
34	7821.5
35	8411.0
36	9511.0
37	10611.0
38	12620.0
39	14629.0
40	17057.5
41	19486.0
42	23317.0
43	27148.0
44	27148.0
45	33344.5
46	39541.0
47	45845.0
48	52149.0
49	53317.0
50	54485.0
51	49861.5
52	45238.0
53	38593.0
54	31948.0
55	28945.5
56	25943.0
57	25943.0
58	23424.5
59	20906.0
60	20257.5
61	19609.0
62	18461.5
63	17314.0
64	15100.0
65	12886.0
66	11140.0
67	9394.0
68	8320.5
69	7247.0
70	7247.0
71	6097.5
72	4948.0
73	4233.0
74	3518.0
75	2797.5
76	2077.0
77	1643.5
78	1210.0
79	925.0
80	640.0
81	640.0
82	458.0
83	276.0
84	213.5
85	151.0
86	93.0
87	35.0
88	29.5
89	24.0
90	13.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	472254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.83684480278312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48283431961039	25.20264105344065
2	10.440500123524366	7.065471646862638
3	3.919304462123463	3.978506904591809
4	2.0597681716533476	2.7878422381578667
5	1.2465529752075057	2.108970978027196
6	0.8823145726110273	1.7912844756403947
7	0.6636052312821675	1.5718015054846788
8	0.5387558240450038	1.4583837763845058
9	0.43458802226859283	1.3234578716385748
>10	4.864763634721125	37.314710525804884
>50	0.4007473526691326	8.519113013260815
>100	0.05869213196571548	3.5345755902013587
>500	0.0037865891590784183	0.8840709849826985
>1k	0.0037865891590784183	2.459169435521922
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4599	0.9738403486259514	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	2016	0.42688891994562245	No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1377	0.2915803783557153	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	1268	0.2684995786165919	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1173	0.24838328526597975	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1083	0.22932574419697876	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	890	0.1884579061267877	No Hit
AAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	721	0.15267207900833027	No Hit
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	641	0.13573204250255158	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGT	635	0.1344615397646182	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	633	0.1340380388519737	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	620	0.1312852829197847	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	493	0.10439297496686105	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3294837100373951	0.0
2	0.0	0.0	0.0	1.3971295108140958	0.0
3	0.0	0.0	0.0	1.8426524709160748	0.0
4	0.0	0.0	0.0	2.35890008342968	0.0
5	0.0	0.0	0.0	3.906372418232561	0.0
6	0.0	0.0	0.0	5.597199811965595	0.0
7	0.0	0.0	0.0	6.835728230994338	0.0
8	0.0	0.0	0.0	9.389650484696794	0.0
9	0.0	0.0	0.0	10.423416212461937	0.0
10	0.0	0.0	0.0	12.275597453912512	0.0
11	0.0	0.0	0.0	14.238524184019617	0.0
12	0.0	0.0	0.0	15.875990462759447	0.0
13	0.0	0.0	0.0	16.59318925832285	0.0
14	0.0	0.0	0.0	16.89726291360158	0.0
15	0.0	0.0	0.0	17.298953529244855	0.0
16	0.0	0.0	0.0	18.34542428438933	0.0
17	0.0	0.0	0.0	19.467066451528204	0.0
18	0.0	0.0	0.0	21.069593904974866	0.0
19	0.0	0.0	0.0	21.654236914880553	0.0
20	0.0	0.0	0.0	22.249255697146026	0.0
21	0.0	0.0	0.0	22.91817538866796	0.0
22	0.0	0.0	0.0	23.56740228775193	0.0
23	0.0	0.0	0.0	24.283754081490045	0.0
24	0.0	0.0	0.0	24.820329737810585	0.0
25	0.0	0.0	0.0	25.290204000389622	0.0
26	0.0	0.0	0.0	25.696976626984632	0.0
27	0.0	0.0	0.0	26.1452523430188	0.0
28	0.0	0.0	0.0	26.601998077305858	0.0
29	0.0	0.0	0.0	27.050697294252668	0.0
30	0.0	0.0	0.0	27.58833170285482	0.0
31	0.0	0.0	0.0	28.056300211326956	0.0
32	0.0	0.0	0.0	28.499070415496746	0.0
33	0.0	0.0	0.0	28.9405701169286	0.0
34	0.0	0.0	0.0	29.364706280942034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	30	4.8614147E-6	40.000004	31
GGCGAAT	30	4.8614147E-6	40.000004	8
CTTAACG	20	0.0012550177	40.0	1
AGACTAT	20	0.0012550177	40.0	25
TCGTTGA	20	0.0012550177	40.0	24
GGTACAA	25	7.787672E-5	40.0	8
GGTCGAT	20	0.0012550177	40.0	8
TTATGAA	20	0.0012550177	40.0	14
TAAACGG	40	1.924127E-8	40.0	2
TGAACGG	55	5.456968E-12	40.0	2
GTGATAG	25	7.787672E-5	40.0	1
ATAGACG	25	7.787672E-5	40.0	1
TTCCGCA	20	0.0012550177	40.0	1
GCTTGCG	20	0.0012550177	40.0	1
ATCGTTG	20	0.0012550177	40.0	23
TATTCGG	20	0.0012550177	40.0	2
GTCGTGT	20	0.0012550177	40.0	32
GATATAC	20	0.0012550177	40.0	9
TGCCTAA	20	0.0012550177	40.0	23
TTAACGG	50	7.8216544E-11	40.0	2
>>END_MODULE