FastQCFastQC Report
Sat 18 Jun 2016
SRR3553824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699861
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60180.8598850343139567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25910.37021637153663367No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT7440.10630682378357989No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCG200.001255432540.01
CTATACG200.001255432540.01
GCCGCAC353.0541014E-740.029
TCGGAAT200.001255432540.031
TATCTCG200.001255432540.01
AATTGCG304.8647835E-640.01
GAAACGC200.001255432540.015
GGACGTA200.001255432540.08
TCCGTCG200.001255432540.031
TTCGAAG353.0541014E-740.01
CAATACG200.001255432540.01
CGTTTTT29000.037.6551741
TCTACGG650.036.9230772
GCGATCT2250.036.4444439
CGTACGG503.421519E-936.02
GCGCGAC1450.035.862079
ATAGCGG900.035.5555572
ACGCTAG455.437323E-835.5555571
TAGGGCC2000.035.05
ACGTATG408.6934597E-735.01
Produced by FastQC (version 0.11.5)