##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	699861
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78954821028747	31.0	31.0	34.0	30.0	34.0
2	31.95472100888605	33.0	31.0	34.0	30.0	34.0
3	31.84467201344267	33.0	31.0	34.0	30.0	34.0
4	35.638151004270846	37.0	35.0	37.0	33.0	37.0
5	35.46450795229338	37.0	35.0	37.0	33.0	37.0
6	35.48413042018343	37.0	35.0	37.0	33.0	37.0
7	35.86719505730424	37.0	35.0	37.0	35.0	37.0
8	35.72033875298095	37.0	35.0	37.0	35.0	37.0
9	37.48085119759495	39.0	37.0	39.0	35.0	39.0
10	36.80047609453877	39.0	37.0	39.0	32.0	39.0
11	36.79577944763317	39.0	37.0	39.0	32.0	39.0
12	36.88483713194477	39.0	37.0	39.0	33.0	39.0
13	35.19742920379904	39.0	35.0	39.0	30.0	39.0
14	37.05928748708672	40.0	36.0	41.0	31.0	41.0
15	37.697612811686895	40.0	37.0	41.0	32.0	41.0
16	37.886085951353195	40.0	37.0	41.0	33.0	41.0
17	37.94104543616518	40.0	37.0	41.0	33.0	41.0
18	37.941656986172966	39.0	37.0	41.0	33.0	41.0
19	37.908467538554085	40.0	37.0	41.0	33.0	41.0
20	37.8349515118002	40.0	36.0	41.0	33.0	41.0
21	37.71537491016073	39.0	36.0	41.0	33.0	41.0
22	37.70104635063248	39.0	36.0	41.0	33.0	41.0
23	37.620358899838685	39.0	36.0	41.0	33.0	41.0
24	37.57852344965643	39.0	36.0	41.0	33.0	41.0
25	37.38938732119664	39.0	36.0	41.0	32.0	41.0
26	37.37116798907211	39.0	36.0	41.0	32.0	41.0
27	37.338877291347856	39.0	36.0	41.0	32.0	41.0
28	37.218049012589645	39.0	35.0	41.0	32.0	41.0
29	37.15655965970385	39.0	35.0	41.0	32.0	41.0
30	36.984865566162426	39.0	35.0	41.0	31.0	41.0
31	36.88088206086637	39.0	35.0	41.0	31.0	41.0
32	36.71522773807942	39.0	35.0	41.0	31.0	41.0
33	36.532841521387816	39.0	35.0	41.0	30.0	41.0
34	36.37462724741056	39.0	35.0	41.0	30.0	41.0
35	36.264686844959215	39.0	35.0	41.0	30.0	41.0
36	36.10545093954371	39.0	35.0	40.0	29.0	41.0
37	36.05740568484313	39.0	35.0	40.0	29.0	41.0
38	35.98600865028913	39.0	35.0	40.0	29.0	41.0
39	35.86842387274044	39.0	35.0	40.0	28.0	41.0
40	35.78372276780675	39.0	35.0	40.0	27.0	41.0
41	35.676149978352846	39.0	35.0	40.0	27.0	41.0
42	35.60571170561011	38.0	35.0	40.0	27.0	41.0
43	35.51827720075843	38.0	35.0	40.0	26.0	41.0
44	35.40252278666764	38.0	34.0	40.0	26.0	41.0
45	35.31496254256202	38.0	34.0	40.0	26.0	41.0
46	34.33857294519912	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	18.0
11	19.0
12	11.0
13	11.0
14	13.0
15	10.0
16	37.0
17	91.0
18	153.0
19	322.0
20	523.0
21	939.0
22	1406.0
23	2136.0
24	3180.0
25	4509.0
26	6727.0
27	9039.0
28	11329.0
29	12787.0
30	14497.0
31	17599.0
32	21895.0
33	28975.0
34	41601.0
35	54396.0
36	68631.0
37	97586.0
38	146373.0
39	155033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.37971540062956	22.56233737842229	22.660071071255576	22.397876149692582
2	28.563957700171894	25.691387289761824	23.49523691132954	22.249418098736747
3	27.51174876153979	26.3835247284818	25.01225243298312	21.092474076995288
4	24.104643636379226	28.564386356719407	24.21266508635286	23.11830492054851
5	21.14319843511783	32.70792343050977	23.0388605737425	23.1100175606299
6	19.87337485586424	39.92992894303297	23.130878845942267	17.065817355160526
7	83.61646098296663	4.951126009307562	8.314508166621657	3.117904841104162
8	84.03711594159412	4.429450990982495	8.239779041838307	3.293654025585081
9	77.05715849290074	7.528494943996023	10.796143805698561	4.618202757404685
10	31.56512507483629	31.187335770960235	20.03083469431787	17.216704459885605
11	25.74882726712876	28.743993450127952	27.02350895392085	18.483670328822438
12	21.53070395407088	25.99987711845638	32.45830243434054	20.011116493132207
13	21.719027063945557	27.696356848002672	32.63933838290746	17.945277705144306
14	21.738173723067867	27.645203833332623	30.84412476191701	19.772497681682506
15	20.701682191177962	27.750081801957815	32.47116213076597	19.077073876098254
16	22.292712410035705	26.665580736746296	31.004870967234922	20.036835885983074
17	22.242273822944842	27.042798498559	29.925513780593576	20.789413897902584
18	22.977134030900423	26.01387989900852	30.23971902992166	20.769267040169407
19	23.525957297234736	27.86896255113515	29.002187577247483	19.602892574382626
20	25.694530771110262	26.204203406104927	28.93088770484425	19.17037811794056
21	25.305024854935482	26.941492667829753	29.247236236909902	18.506246240324863
22	24.3098272371228	25.436050872959058	28.365632604188544	21.888489285729595
23	22.134681029518717	28.018849457249367	28.979040123681703	20.86742938955021
24	21.152200222615633	27.274987461795984	30.742961816703602	20.829850498884777
25	22.207838413627847	28.333912019672482	28.596249826751315	20.861999739948363
26	21.380245505893313	28.444219637899526	28.99704369867731	21.17849115752985
27	22.667215347047485	27.34757330384176	29.083060779211873	20.90215056989888
28	21.136768586905113	28.261754834174212	30.01581742660328	20.58565915231739
29	22.53961858140402	27.239837624899803	28.85901629037766	21.361527503318516
30	22.68050370002043	27.51803572423667	28.749137328698126	21.05232324704477
31	22.440741804444027	28.75885354377512	27.46874022127251	21.331664430508344
32	22.77909470594875	28.14716065047202	28.51166160137513	20.562083042204094
33	22.36344074037559	29.017333441926326	26.73888100637127	21.88034481132682
34	22.014657196214678	29.269240606348973	27.500889462336094	21.215212735100256
35	23.512383173230113	28.096722063381158	27.30285013738442	21.088044626004308
36	22.758804962699735	30.878417285718164	25.527926259642985	20.834851491939112
37	22.89483197377765	30.199853971002817	26.5299823822159	20.37533167300364
38	22.914693060479152	29.368546039856486	26.700444802610807	21.016316097053558
39	22.59734432980263	28.22960559311063	26.788890936914616	22.38415914017212
40	23.026286648348744	27.81209411583157	28.383350408152474	20.77826882766721
41	21.612577354646138	28.37291976549629	27.76980000314348	22.24470287671409
42	22.587771000241478	28.292760991111095	27.20825992589957	21.91120808274786
43	21.913351365485433	27.76765672040591	28.429645315284034	21.889346598824623
44	22.411736044728883	28.428930887704844	27.452308386951124	21.70702468061515
45	22.755661481351297	27.003933638251027	28.04485462113191	22.19555025926577
46	22.736371936713148	28.163449599277573	27.11767050885819	21.982507955151096
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2798.0
1	2508.5
2	2219.0
3	6332.0
4	10445.0
5	7129.0
6	3813.0
7	3813.0
8	3547.5
9	3282.0
10	3224.5
11	3167.0
12	3116.5
13	3066.0
14	3030.0
15	2994.0
16	2874.5
17	2755.0
18	2829.0
19	2903.0
20	2903.0
21	3010.0
22	3117.0
23	3819.0
24	4521.0
25	5352.0
26	6183.0
27	7457.5
28	8732.0
29	10454.0
30	12176.0
31	12176.0
32	14128.0
33	16080.0
34	18443.5
35	20807.0
36	24350.5
37	27894.0
38	29816.0
39	31738.0
40	35473.0
41	39208.0
42	41398.5
43	43589.0
44	43589.0
45	48246.0
46	52903.0
47	56781.0
48	60659.0
49	61458.0
50	62257.0
51	59857.5
52	57458.0
53	52719.0
54	47980.0
55	44098.5
56	40217.0
57	40217.0
58	36704.5
59	33192.0
60	30358.0
61	27524.0
62	24011.5
63	20499.0
64	17511.5
65	14524.0
66	12550.5
67	10577.0
68	9221.0
69	7865.0
70	7865.0
71	6432.5
72	5000.0
73	4093.5
74	3187.0
75	2583.5
76	1980.0
77	1562.5
78	1145.0
79	969.5
80	794.0
81	794.0
82	568.0
83	342.0
84	229.5
85	117.0
86	109.0
87	101.0
88	70.5
89	40.0
90	24.5
91	9.0
92	6.5
93	4.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	699861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.710566095389694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69745634653106	27.05475342271425
2	9.142988478501028	6.71288565698797
3	3.7181646243200257	4.094877845839203
4	2.2827681337587	3.352068418191926
5	1.584805078196824	2.908954578572687
6	1.1986502750577177	2.6401878088658037
7	0.9974775021132748	2.5632574638995513
8	0.8148452904359594	2.393074551365304
9	0.7011005089849972	2.3164016917144585
>10	5.731543323480636	40.21016830077168
>50	0.10377169327739363	2.411410368421993
>100	0.02409679722392119	1.743674672251198
>500	0.0015546320789626572	0.36996550406246265
>1k	3.886580197406643E-4	0.36968014694401874
>5k	3.886580197406643E-4	0.8586395693975705
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6018	0.8598850343139567	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2591	0.37021637153663367	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT	744	0.10630682378357989	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8577103167629E-4	0.0	0.0	0.12245288707329027	0.0
2	2.8577103167629E-4	0.0	0.0	0.37793218939189355	0.0
3	2.8577103167629E-4	0.0	0.0	0.5639691310131584	0.0
4	2.8577103167629E-4	0.0	0.0	0.7317167266071406	0.0
5	2.8577103167629E-4	0.0	0.0	1.1776624215379912	0.0
6	2.8577103167629E-4	0.0	0.0	1.84865280391392	0.0
7	2.8577103167629E-4	0.0	0.0	2.3328918170893935	0.0
8	2.8577103167629E-4	0.0	0.0	3.2835091539605723	0.0
9	2.8577103167629E-4	0.0	0.0	3.71273724353836	0.0
10	2.8577103167629E-4	0.0	0.0	4.522469461793127	0.0
11	2.8577103167629E-4	0.0	0.0	5.410502942727199	0.0
12	2.8577103167629E-4	0.0	0.0	6.1190722157685595	0.0
13	2.8577103167629E-4	0.0	0.0	6.483858937703344	0.0
14	2.8577103167629E-4	0.0	0.0	6.661036977342643	0.0
15	2.8577103167629E-4	0.0	0.0	6.874936594552347	0.0
16	2.8577103167629E-4	0.0	0.0	7.329598305949324	0.0
17	2.8577103167629E-4	0.0	0.0	7.868562471690807	0.0
18	2.8577103167629E-4	0.0	0.0	8.511118636414945	0.0
19	2.8577103167629E-4	0.0	0.0	8.825895427806378	0.0
20	2.8577103167629E-4	0.0	0.0	9.138957593007754	0.0
21	2.8577103167629E-4	0.0	0.0	9.518175752042191	0.0
22	2.8577103167629E-4	0.0	0.0	9.934544145194545	0.0
23	2.8577103167629E-4	0.0	0.0	10.371202281595917	0.0
24	2.8577103167629E-4	0.0	0.0	10.723986620200296	0.0
25	2.8577103167629E-4	0.0	0.0	11.023188890365372	0.0
26	2.8577103167629E-4	0.0	0.0	11.295814454584553	0.0
27	2.8577103167629E-4	0.0	0.0	11.617735521767894	0.0
28	2.8577103167629E-4	0.0	0.0	11.938227733792854	0.0
29	2.8577103167629E-4	0.0	0.0	12.281867399383591	0.0
30	2.8577103167629E-4	0.0	0.0	12.707523351065426	0.0
31	2.8577103167629E-4	0.0	0.0	13.063165399986568	0.0
32	2.8577103167629E-4	0.0	0.0	13.422665357835342	0.0
33	2.8577103167629E-4	0.0	0.0	13.743014684344462	0.0
34	2.8577103167629E-4	0.0	0.0	14.074937737636473	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	20	0.0012554325	40.0	1
CTATACG	20	0.0012554325	40.0	1
GCCGCAC	35	3.0541014E-7	40.0	29
TCGGAAT	20	0.0012554325	40.0	31
TATCTCG	20	0.0012554325	40.0	1
AATTGCG	30	4.8647835E-6	40.0	1
GAAACGC	20	0.0012554325	40.0	15
GGACGTA	20	0.0012554325	40.0	8
TCCGTCG	20	0.0012554325	40.0	31
TTCGAAG	35	3.0541014E-7	40.0	1
CAATACG	20	0.0012554325	40.0	1
CGTTTTT	2900	0.0	37.655174	1
TCTACGG	65	0.0	36.923077	2
GCGATCT	225	0.0	36.444443	9
CGTACGG	50	3.421519E-9	36.0	2
GCGCGAC	145	0.0	35.86207	9
ATAGCGG	90	0.0	35.555557	2
ACGCTAG	45	5.437323E-8	35.555557	1
TAGGGCC	200	0.0	35.0	5
ACGTATG	40	8.6934597E-7	35.0	1
>>END_MODULE