FastQCFastQC Report
Sat 18 Jun 2016
SRR3553823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425540
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39410.9261174037693283No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.3231188607416459No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10000.2349955350848334No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG8840.20773605301499273No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT8210.1929313343046482No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.1891714057432909No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT8010.18823142360295156No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA6990.16426187902429853No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCG6800.1597969638576867No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT6750.15862198618226256No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC5750.1351224326737792No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACT5630.1323024862527612No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGT5470.12854255769140385No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA5380.12642759787564037No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA4530.10645297739342952No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA200.001254877940.038
GTACCTC200.001254877940.040
TAGGTAC200.001254877940.017
CTATACG200.001254877940.01
GTTAGCG200.001254877940.01
TAAGTAG257.786369E-540.01
GCGTTAG451.2169039E-940.01
TCTAGAT200.001254877940.031
GCCGCAT200.001254877940.019
AATGCGT200.001254877940.020
CTAACGG257.786369E-540.02
CGTAGTA353.050336E-740.031
TGCGATC200.001254877940.034
CTACGCG257.786369E-540.01
CTGTTCG200.001254877940.032
AGATTAT257.786369E-540.011
CATACGC200.001254877940.013
TCGTACC200.001254877940.038
TCCCGAT200.001254877940.015
CTCGATG200.001254877940.01
Produced by FastQC (version 0.11.5)