##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425540
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.832525732011092	31.0	31.0	34.0	30.0	34.0
2	31.98230483620811	33.0	31.0	34.0	30.0	34.0
3	31.943199229214645	33.0	31.0	34.0	30.0	34.0
4	35.69921511491282	37.0	35.0	37.0	33.0	37.0
5	35.50354138271373	37.0	35.0	37.0	33.0	37.0
6	35.52482022841566	37.0	35.0	37.0	33.0	37.0
7	35.79308878131315	37.0	35.0	37.0	35.0	37.0
8	35.55046764111482	37.0	35.0	37.0	35.0	37.0
9	37.3561004840908	39.0	37.0	39.0	34.0	39.0
10	36.87932979273394	39.0	37.0	39.0	32.0	39.0
11	36.86615124312638	39.0	37.0	39.0	33.0	39.0
12	36.90063448794473	39.0	37.0	39.0	33.0	39.0
13	35.21342764487475	39.0	35.0	39.0	30.0	39.0
14	37.0231400103398	40.0	36.0	41.0	31.0	41.0
15	37.64112891855055	40.0	36.0	41.0	32.0	41.0
16	37.86036800300794	40.0	37.0	41.0	33.0	41.0
17	37.893398975419466	40.0	37.0	41.0	33.0	41.0
18	37.91040560229356	39.0	37.0	41.0	33.0	41.0
19	37.86222681769046	40.0	37.0	41.0	33.0	41.0
20	37.80230295624383	40.0	36.0	41.0	33.0	41.0
21	37.66895473985994	39.0	36.0	41.0	33.0	41.0
22	37.658126145603234	39.0	36.0	41.0	33.0	41.0
23	37.55867838511068	39.0	36.0	41.0	33.0	41.0
24	37.52455233350566	39.0	36.0	41.0	33.0	41.0
25	37.30094468205104	39.0	35.0	41.0	32.0	41.0
26	37.304939606147485	39.0	35.0	41.0	32.0	41.0
27	37.30143347276402	39.0	35.0	41.0	32.0	41.0
28	37.14685810969591	39.0	35.0	41.0	32.0	41.0
29	37.031355454246366	39.0	35.0	41.0	31.0	41.0
30	36.90300324293838	39.0	35.0	41.0	31.0	41.0
31	36.81781031160408	39.0	35.0	41.0	31.0	41.0
32	36.68781548150585	39.0	35.0	40.0	31.0	41.0
33	36.58133430464821	39.0	35.0	40.0	30.0	41.0
34	36.49407576256051	39.0	35.0	40.0	30.0	41.0
35	36.345969826573295	39.0	35.0	40.0	30.0	41.0
36	36.19425200921182	39.0	35.0	40.0	30.0	41.0
37	36.15004464915167	39.0	35.0	40.0	30.0	41.0
38	36.0164755369648	38.0	35.0	40.0	29.0	41.0
39	35.9522277576726	38.0	35.0	40.0	29.0	41.0
40	35.78707994548103	38.0	35.0	40.0	29.0	41.0
41	35.65362833106171	38.0	35.0	40.0	28.0	41.0
42	35.56364384076703	38.0	35.0	40.0	28.0	41.0
43	35.51039620247215	38.0	35.0	40.0	28.0	41.0
44	35.4524956525826	38.0	34.0	40.0	28.0	41.0
45	35.38067161723927	38.0	34.0	40.0	27.0	41.0
46	34.33459134276449	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	13.0
10	20.0
11	22.0
12	12.0
13	15.0
14	12.0
15	17.0
16	30.0
17	54.0
18	91.0
19	204.0
20	399.0
21	607.0
22	947.0
23	1425.0
24	2034.0
25	2710.0
26	3404.0
27	4349.0
28	5437.0
29	6601.0
30	8305.0
31	10602.0
32	13900.0
33	18754.0
34	28033.0
35	36456.0
36	44118.0
37	62042.0
38	88546.0
39	86376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.21675988156225	23.733374065892747	21.743666870329463	20.306199182215536
2	24.810593598721624	29.55609343422475	23.01099779104197	22.622315176011657
3	27.00521690087888	29.319687925929404	23.62363115100813	20.05146402218358
4	24.881092259247076	28.603421534990837	22.228697654744558	24.28678855101753
5	21.363444094562205	33.452319405931284	20.52357005216901	24.6606664473375
6	19.34365747050806	41.00907082765428	21.322084880387273	18.325186821450394
7	80.85914367627015	7.0021619589227795	7.983973304507215	4.154721060299854
8	79.49264463975184	7.5804859707665555	7.946609014428725	4.980260375052874
9	72.39671946233021	9.297128354561265	11.199182215537904	7.106969967570616
10	32.8493208629036	29.94900596888659	18.13578042017202	19.06589274803779
11	26.95516285190581	28.274897776942236	23.82196738261973	20.947971988532217
12	22.73041312215068	25.010574799078817	30.9451520421112	21.313860036659303
13	22.48578277012737	27.040936222211776	31.191427362880102	19.28185364478075
14	20.69206185082483	28.652535601823566	28.568407200263195	22.086995347088408
15	19.72529021948583	27.71678338111576	31.188842411994173	21.369083987404238
16	21.461672228227663	26.53405085303379	29.238614466325142	22.765662452413405
17	20.907317760962542	26.628049067067728	27.828876251351225	24.63575692061851
18	22.5301969262584	26.212576961037744	27.684119001738967	23.57310711096489
19	23.79870282464633	28.75922357475208	26.012595760680547	21.42947783992104
20	25.3858626686093	26.43182779527189	26.200357193213332	21.981952342905483
21	25.39385251680218	27.648164684870984	26.843774968275603	20.114207830051228
22	22.545941627109084	26.42031301405273	25.715326408798234	25.318418950039952
23	21.87714433425765	28.335291629459043	26.577995018094658	23.209569018188656
24	22.050571039150256	26.731212106969966	27.564506274380783	23.65371057949899
25	22.36687502937444	28.775673262208016	25.759505569394182	23.09794613902336
26	20.020444611552378	28.86567655214551	27.073130610518398	24.04074822578371
27	22.33092071250646	27.685528974949474	26.549560558349388	23.43398975419467
28	21.641443812567562	28.584856887719134	27.38473469004089	22.388964609672417
29	22.649809653616582	28.617521267095924	24.94994595102693	23.782723128260564
30	23.92301546270621	28.286647553696483	24.83033322366875	22.96000375992856
31	24.384546693612823	29.104197020256617	23.417305071203646	23.093951214926918
32	22.63429994830098	29.362927104385015	24.728345161441933	23.274427785872067
33	23.050712036471307	28.43704469615077	23.677915119612727	24.83432814776519
34	24.060252855195753	27.786342059500868	24.848192884335198	23.30521220096818
35	23.920900502890447	26.707947548996568	24.592282746627813	24.778869201485172
36	24.312873055411945	29.288433519763124	24.349767354420266	22.04892607040466
37	23.968134605442497	27.557926399398415	25.69488179724585	22.77905719791324
38	23.81750246745312	26.912393664520373	26.99887202143159	22.271231846594915
39	25.220895802979744	24.095032194388306	26.500211495981574	24.183860506650372
40	24.639516849179866	24.99013018752644	29.009963810687594	21.360389152606103
41	21.91121868684495	25.91295765380458	28.436574705080602	23.73924895426987
42	22.49353762278517	26.927198383230717	28.35033134370447	22.228932650279646
43	22.49471260046059	24.663486393758518	29.021478591906753	23.820322413874138
44	24.16506086384359	25.423226958687785	26.80171076749542	23.61000140997321
45	24.11618179254594	24.134276448747475	26.624759129576535	25.12478262913005
46	24.784039103257037	25.77219532828876	26.9321332894675	22.5116322789867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4314.0
1	3734.5
2	3155.0
3	3217.0
4	3279.0
5	2455.5
6	1632.0
7	1632.0
8	1492.5
9	1353.0
10	1364.5
11	1376.0
12	1380.0
13	1384.0
14	1398.5
15	1413.0
16	1306.0
17	1199.0
18	1239.0
19	1279.0
20	1279.0
21	1407.5
22	1536.0
23	1900.5
24	2265.0
25	2694.0
26	3123.0
27	3371.0
28	3619.0
29	5284.5
30	6950.0
31	6950.0
32	7308.0
33	7666.0
34	8546.0
35	9426.0
36	10708.0
37	11990.0
38	14194.0
39	16398.0
40	17214.5
41	18031.0
42	20536.5
43	23042.0
44	23042.0
45	26859.5
46	30677.0
47	35153.0
48	39629.0
49	39697.0
50	39765.0
51	38012.5
52	36260.0
53	32405.0
54	28550.0
55	26695.0
56	24840.0
57	24840.0
58	23012.5
59	21185.0
60	19944.0
61	18703.0
62	17712.5
63	16722.0
64	14984.0
65	13246.0
66	11748.0
67	10250.0
68	8842.5
69	7435.0
70	7435.0
71	6401.5
72	5368.0
73	4398.0
74	3428.0
75	2964.5
76	2501.0
77	1891.5
78	1282.0
79	971.0
80	660.0
81	660.0
82	497.0
83	334.0
84	255.5
85	177.0
86	119.0
87	61.0
88	44.5
89	28.0
90	16.5
91	5.0
92	4.5
93	4.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	425540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.01292156927223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38260861351034	24.132175372314517
2	9.219866770243359	5.903097435898797
3	3.4491107515137216	3.3124833591582714
4	1.8081211187852078	2.3153295825366245
5	1.1619552245563567	1.8598790735364374
6	0.8768883768417804	1.684305529970541
7	0.6871687448185827	1.5398795392912772
8	0.5715991202255848	1.4638846243877321
9	0.45693597066023167	1.3165069851832745
>10	5.926630617856353	42.6181455502361
>50	0.4025517610586993	8.025126177919018
>100	0.04627876085163879	2.5594993061147107
>500	0.008815002066978815	2.0195681772246536
>1k	0.0014691670111631361	1.2501192862280228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3941	0.9261174037693283	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1375	0.3231188607416459	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1000	0.2349955350848334	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	884	0.20773605301499273	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	821	0.1929313343046482	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	805	0.1891714057432909	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	801	0.18823142360295156	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA	699	0.16426187902429853	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCG	680	0.1597969638576867	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	675	0.15862198618226256	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC	575	0.1351224326737792	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACT	563	0.1323024862527612	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGT	547	0.12854255769140385	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	538	0.12642759787564037	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA	453	0.10645297739342952	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.349955350848334E-4	0.0	0.0	0.1746016825680312	0.0
2	2.349955350848334E-4	0.0	0.0	0.739060957841801	0.0
3	2.349955350848334E-4	0.0	0.0	0.9851012830756216	0.0
4	2.349955350848334E-4	0.0	0.0	1.3093951214926915	0.0
5	2.349955350848334E-4	0.0	0.0	2.3516003195939277	0.0
6	2.349955350848334E-4	0.0	0.0	3.3461014240729425	0.0
7	2.349955350848334E-4	0.0	0.0	4.11124688630916	0.0
8	2.349955350848334E-4	0.0	0.0	5.540489730695117	0.0
9	2.349955350848334E-4	0.0	0.0	6.2071720637307894	0.0
10	2.349955350848334E-4	0.0	0.0	7.560041359214175	0.0
11	2.349955350848334E-4	0.0	0.0	8.841942003101941	0.0
12	2.349955350848334E-4	0.0	0.0	10.070028669455281	0.0
13	2.349955350848334E-4	0.0	0.0	10.533439864642572	0.0
14	2.349955350848334E-4	0.0	0.0	10.738120975701461	0.0
15	2.349955350848334E-4	0.0	0.0	11.038210274004793	0.0
16	2.349955350848334E-4	0.0	0.0	11.698077736523006	0.0
17	2.349955350848334E-4	0.0	0.0	12.39789444000564	0.0
18	2.349955350848334E-4	0.0	0.0	13.429289843492974	0.0
19	2.349955350848334E-4	0.0	0.0	13.833717159373972	0.0
20	2.349955350848334E-4	0.0	0.0	14.259764064482775	0.0
21	2.349955350848334E-4	0.0	0.0	14.713305447196504	0.0
22	2.349955350848334E-4	0.0	0.0	15.160971941533111	0.0
23	2.349955350848334E-4	0.0	0.0	15.645297739342952	0.0
24	2.349955350848334E-4	0.0	0.0	15.987216242891385	0.0
25	2.349955350848334E-4	0.0	0.0	16.307750152747097	0.0
26	2.349955350848334E-4	0.0	0.0	16.575175071673637	0.0
27	2.349955350848334E-4	0.0	0.0	16.89758894581003	0.0
28	2.349955350848334E-4	0.0	0.0	17.22775767260422	0.0
29	2.349955350848334E-4	0.0	0.0	17.548761573530104	0.0
30	2.349955350848334E-4	0.0	0.0	17.980448371480943	0.0
31	2.349955350848334E-4	0.0	0.0	18.30074728580157	0.0
32	2.349955350848334E-4	0.0	0.0	18.631620999201015	0.0
33	2.349955350848334E-4	0.0	0.0	18.979414391126568	0.0
34	2.349955350848334E-4	0.0	0.0	19.274803778728206	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	0.0012548779	40.0	38
GTACCTC	20	0.0012548779	40.0	40
TAGGTAC	20	0.0012548779	40.0	17
CTATACG	20	0.0012548779	40.0	1
GTTAGCG	20	0.0012548779	40.0	1
TAAGTAG	25	7.786369E-5	40.0	1
GCGTTAG	45	1.2169039E-9	40.0	1
TCTAGAT	20	0.0012548779	40.0	31
GCCGCAT	20	0.0012548779	40.0	19
AATGCGT	20	0.0012548779	40.0	20
CTAACGG	25	7.786369E-5	40.0	2
CGTAGTA	35	3.050336E-7	40.0	31
TGCGATC	20	0.0012548779	40.0	34
CTACGCG	25	7.786369E-5	40.0	1
CTGTTCG	20	0.0012548779	40.0	32
AGATTAT	25	7.786369E-5	40.0	11
CATACGC	20	0.0012548779	40.0	13
TCGTACC	20	0.0012548779	40.0	38
TCCCGAT	20	0.0012548779	40.0	15
CTCGATG	20	0.0012548779	40.0	1
>>END_MODULE