FastQCFastQC Report
Sat 18 Jun 2016
SRR3553822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665604
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42630.6404709106315467No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.29627225797921886No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10300.15474666618590033No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT9530.14317822609239125No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT9250.13897152060384252No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC8260.12409781191218804No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT7890.11853895108803432No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7720.1159848798985583No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7710.11583464041682442No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA7290.10952458218400131No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGT6800.102162847579041No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGA200.00125538840.028
ACGCCTA200.00125538840.01
ACGTTAG200.00125538840.01
CCGCTTA200.00125538840.014
CGTTCAT353.0537922E-740.017
GCCGATA555.456968E-1240.09
TATTAGT304.8644233E-640.030
CATACCC257.791116E-540.028
ACCGTAA200.00125538840.017
CGCAACT200.00125538840.013
TCGAATA200.00125538840.027
TACCGTA200.00125538840.016
TATACGG950.037.8947332
CGCATGG1200.036.6666642
TAGCACG601.4551915E-1136.6666641
GTATACG601.4551915E-1136.6666641
TACGGGT552.1645974E-1036.3636364
GCGATAC552.1645974E-1036.3636369
ATAACGG552.1645974E-1036.3636362
TACAGCG552.1645974E-1036.3636361