##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665604
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86761497827537	31.0	31.0	34.0	30.0	34.0
2	32.02131147048395	33.0	31.0	34.0	30.0	34.0
3	31.99116441607923	33.0	31.0	34.0	30.0	34.0
4	35.73617496289085	37.0	35.0	37.0	35.0	37.0
5	35.533299078731496	37.0	35.0	37.0	33.0	37.0
6	35.54266350562797	37.0	35.0	37.0	33.0	37.0
7	35.82831984182787	37.0	35.0	37.0	35.0	37.0
8	35.629769352347644	37.0	35.0	37.0	35.0	37.0
9	37.40584341440256	39.0	37.0	39.0	35.0	39.0
10	36.91268081321627	39.0	37.0	39.0	33.0	39.0
11	36.89378819838823	39.0	37.0	39.0	33.0	39.0
12	36.924037415640534	39.0	37.0	39.0	33.0	39.0
13	35.21629226987819	39.0	35.0	39.0	30.0	39.0
14	37.05012740308051	40.0	36.0	41.0	31.0	41.0
15	37.67156898095564	40.0	36.0	41.0	32.0	41.0
16	37.87835559882453	40.0	37.0	41.0	33.0	41.0
17	37.925793715182	40.0	37.0	41.0	33.0	41.0
18	37.96148761125234	40.0	37.0	41.0	33.0	41.0
19	37.92786251284548	40.0	37.0	41.0	33.0	41.0
20	37.887308369541046	40.0	37.0	41.0	33.0	41.0
21	37.75628301512611	40.0	36.0	41.0	33.0	41.0
22	37.722644395165894	39.0	36.0	41.0	33.0	41.0
23	37.64765386025324	39.0	36.0	41.0	33.0	41.0
24	37.583971851130705	39.0	36.0	41.0	33.0	41.0
25	37.37952446199242	39.0	36.0	41.0	32.0	41.0
26	37.3807188658722	39.0	36.0	41.0	32.0	41.0
27	37.38721221627274	39.0	36.0	41.0	32.0	41.0
28	37.228589371458106	39.0	35.0	41.0	32.0	41.0
29	37.1447301999387	39.0	35.0	41.0	32.0	41.0
30	37.02440940859731	39.0	35.0	41.0	31.0	41.0
31	37.00165563908871	39.0	35.0	41.0	31.0	41.0
32	36.880039783414766	39.0	35.0	41.0	31.0	41.0
33	36.79485399727165	39.0	35.0	41.0	31.0	41.0
34	36.67630753420953	39.0	35.0	41.0	31.0	41.0
35	36.59603908630357	39.0	35.0	41.0	30.0	41.0
36	36.466549179391954	39.0	35.0	40.0	30.0	41.0
37	36.419551264715956	39.0	35.0	40.0	30.0	41.0
38	36.28574197270449	39.0	35.0	40.0	30.0	41.0
39	36.18251542959477	39.0	35.0	40.0	30.0	41.0
40	36.054246969669656	38.0	35.0	40.0	30.0	41.0
41	35.929126026886856	38.0	35.0	40.0	29.0	41.0
42	35.846519852645116	38.0	35.0	40.0	29.0	41.0
43	35.80216164566319	38.0	35.0	40.0	29.0	41.0
44	35.70650717243286	38.0	35.0	40.0	29.0	41.0
45	35.612583458032105	38.0	35.0	40.0	29.0	41.0
46	34.576254048954034	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	23.0
10	28.0
11	20.0
12	20.0
13	13.0
14	15.0
15	24.0
16	29.0
17	73.0
18	144.0
19	264.0
20	504.0
21	830.0
22	1306.0
23	1918.0
24	2815.0
25	3827.0
26	4897.0
27	6331.0
28	8041.0
29	10010.0
30	12266.0
31	15947.0
32	20900.0
33	28440.0
34	42301.0
35	55229.0
36	68065.0
37	98101.0
38	141881.0
39	141335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.73717706023401	23.429997415880916	22.393344991917115	20.43948053196796
2	25.633710133953524	28.239313465664267	23.239343513560616	22.887632886821592
3	28.409685037950496	26.980306608734324	23.04523410316044	21.56477425015475
4	25.21589413525159	28.701149632514227	21.479287985048167	24.603668247186015
5	21.399811299210942	34.08813648956436	20.094680921388694	24.417371289835998
6	20.657478019963822	40.08118941592899	21.383585435183683	17.877747128923506
7	82.3479125726408	6.702633998593758	7.018888107643583	3.9305653211218683
8	81.84656342209482	6.584095047505724	6.878263952740668	4.691077577658788
9	74.1171928053317	9.057938353735855	10.374186453206411	6.450682387726035
10	33.75791010871329	29.133388621462615	17.4760067547671	19.632694515057
11	27.929970372774203	28.70355346422197	23.53726840583891	19.82920775716492
12	23.367347551997884	25.533800878600488	29.72518194001238	21.373669629389248
13	23.369000186296958	27.998028857999653	29.21692177330665	19.41604918239674
14	21.846923996850983	28.197396650260515	27.85244680019952	22.103232552688986
15	20.769105954892098	27.027932524443965	30.815920577400373	21.387040943263564
16	22.607886971833103	26.938990751257503	28.373477322852626	22.079644954056764
17	21.962007439859136	26.670512797399056	27.15203033635615	24.21544942638566
18	23.888077595687527	26.15699424883264	27.268315695218178	22.686612460261657
19	24.79266951520724	27.395568536246778	26.093743427022677	21.71801852152331
20	27.06999957932945	25.996238003377382	25.254806161020664	21.6789562562725
21	26.951009909796213	27.343585675566857	25.174728517256504	20.530675897380423
22	24.72250767723752	26.176224902494578	24.865535663848174	24.235731756419735
23	22.99775842693253	27.553620471030822	25.912404372569874	23.53621672946677
24	21.853534534047274	27.022223424138076	27.279583656348215	23.84465838546643
25	22.86028930114603	28.587718823805147	25.652339829688525	22.899652045360305
26	21.87516901941695	27.673661816936196	26.646324240839895	23.804844922806954
27	23.84420766702123	26.00660452761702	26.53079007938654	23.618397725975203
28	22.370959309138765	27.594034891617238	27.18898924886269	22.846016550381307
29	23.5266014026358	27.495327552118077	25.476709875541616	23.50136116970451
30	23.655356638481738	26.84599251206423	26.00585333020835	23.492797519245677
31	23.710194049314605	28.028076754346426	24.247750914958445	24.01397828138052
32	22.768492977806623	27.89195978389553	25.681486289144896	23.65806094915295
33	23.461397467563298	27.005546841665616	24.361181723667528	25.171873967103565
34	22.21906719310581	27.140612135744373	26.359216591246447	24.281104079903365
35	23.87080005528813	26.33562899261423	25.751798366596358	24.04177258550129
36	23.507971706900797	28.13219271518801	25.094951352455812	23.264884225455376
37	24.26112222883276	27.795656276104108	25.312347882524744	22.630873612538384
38	22.169638403615362	28.961214175395583	25.62589768090336	23.243249740085698
39	23.53666744791197	26.049723258874646	26.074512773360738	24.339096519852646
40	23.816713841863933	25.994284890114844	28.34342942650585	21.845571841515376
41	21.709905589509678	26.449360280286776	27.96197138238352	23.878762747820026
42	22.24731221567178	26.781089055955192	28.12483098058305	22.84676774778998
43	22.827687333609774	25.208682640128366	28.492316752904127	23.471313273357733
44	23.238592316151944	25.27043106712099	26.872735139812864	24.6182414769142
45	23.98257822969814	24.974759767068708	26.566997794484408	24.475664208748746
46	23.422635681275956	26.16615885721841	26.78950246693229	23.621702994573347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4613.0
1	4088.0
2	3563.0
3	4091.0
4	4619.0
5	3394.5
6	2170.0
7	2170.0
8	2025.0
9	1880.0
10	1910.5
11	1941.0
12	2045.0
13	2149.0
14	2257.0
15	2365.0
16	2297.5
17	2230.0
18	2331.5
19	2433.0
20	2433.0
21	2239.5
22	2046.0
23	2713.5
24	3381.0
25	4085.0
26	4789.0
27	5415.0
28	6041.0
29	8010.0
30	9979.0
31	9979.0
32	11776.5
33	13574.0
34	15298.5
35	17023.0
36	18117.5
37	19212.0
38	22207.0
39	25202.0
40	27717.5
41	30233.0
42	33410.5
43	36588.0
44	36588.0
45	41541.0
46	46494.0
47	51295.0
48	56096.0
49	57468.5
50	58841.0
51	54902.0
52	50963.0
53	48220.0
54	45477.0
55	42953.5
56	40430.0
57	40430.0
58	37967.5
59	35505.0
60	33244.0
61	30983.0
62	28740.5
63	26498.0
64	23951.5
65	21405.0
66	19727.5
67	18050.0
68	16146.5
69	14243.0
70	14243.0
71	11870.5
72	9498.0
73	7614.0
74	5730.0
75	4844.0
76	3958.0
77	3262.5
78	2567.0
79	2098.5
80	1630.0
81	1630.0
82	1170.0
83	710.0
84	465.5
85	221.0
86	206.0
87	191.0
88	117.0
89	43.0
90	37.5
91	32.0
92	18.5
93	5.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	665604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.77813898378976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65484635019767	20.45994558745471
2	9.730971879752834	5.406165786462482
3	3.797791188266872	3.164867143772677
4	1.9836000338987452	2.204028697195577
5	1.2177631730584182	1.6913597335278778
6	0.9057044810342224	1.5095270971445882
7	0.6762176631064813	1.3148847760345752
8	0.5225464960082925	1.161229535328855
9	0.4532580649977261	1.1331598972527401
>10	6.096212608269655	40.69524003051718
>50	0.8807697299553059	15.772315231794702
>100	0.07430811617488786	3.4064400452505756
>500	0.004371065657346345	0.9781904335965051
>1k	0.0016391496215048794	1.1026460046669682
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4263	0.6404709106315467	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1972	0.29627225797921886	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1030	0.15474666618590033	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	953	0.14317822609239125	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	925	0.13897152060384252	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC	826	0.12409781191218804	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	789	0.11853895108803432	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	772	0.1159848798985583	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	771	0.11583464041682442	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA	729	0.10952458218400131	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGT	680	0.102162847579041	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12935619377287397	0.0
2	0.0	0.0	0.0	0.5608439853125883	0.0
3	0.0	0.0	0.0	0.7611132144638554	0.0
4	0.0	0.0	0.0	1.0154686570393208	0.0
5	0.0	0.0	0.0	1.700410454264097	0.0
6	0.0	0.0	0.0	2.547460652279734	0.0
7	0.0	0.0	0.0	3.138202294457365	0.0
8	0.0	0.0	0.0	4.326897073935854	0.0
9	0.0	0.0	0.0	4.885938185467635	0.0
10	0.0	0.0	0.0	5.864297690518687	0.0
11	0.0	0.0	0.0	6.897344366920872	0.0
12	0.0	0.0	0.0	7.739737141002759	0.0
13	0.0	0.0	0.0	8.095203754785128	0.0
14	0.0	0.0	0.0	8.250551378897963	0.0
15	0.0	0.0	0.0	8.498296284277139	0.0
16	0.0	0.0	0.0	9.038707700073918	0.0
17	0.0	0.0	0.0	9.64958143280389	0.0
18	0.0	0.0	0.0	10.456367449714845	0.0
19	0.0	0.0	0.0	10.78494119626685	0.0
20	0.0	0.0	0.0	11.165497803498777	0.0
21	0.0	0.0	0.0	11.559125245641553	0.0
22	0.0	0.0	0.0	11.968828312329855	0.0
23	0.0	0.0	0.0	12.397611793198358	0.0
24	0.0	0.0	0.0	12.726185539750363	0.0
25	0.0	0.0	0.0	13.049050185996478	0.0
26	0.0	0.0	0.0	13.305058262871016	0.0
27	0.0	0.0	0.0	13.595621420544347	0.0
28	0.0	0.0	0.0	13.888588409925422	0.0
29	0.0	0.0	0.0	14.201387010895367	0.0
30	0.0	0.0	0.0	14.628968575910001	0.0
31	0.0	0.0	0.0	14.954687772309061	0.0
32	0.0	0.0	0.0	15.256518891112433	0.0
33	0.0	0.0	0.0	15.564810307630363	0.0
34	0.0	0.0	0.0	15.856274902194098	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGA	20	0.001255388	40.0	28
ACGCCTA	20	0.001255388	40.0	1
ACGTTAG	20	0.001255388	40.0	1
CCGCTTA	20	0.001255388	40.0	14
CGTTCAT	35	3.0537922E-7	40.0	17
GCCGATA	55	5.456968E-12	40.0	9
TATTAGT	30	4.8644233E-6	40.0	30
CATACCC	25	7.791116E-5	40.0	28
ACCGTAA	20	0.001255388	40.0	17
CGCAACT	20	0.001255388	40.0	13
TCGAATA	20	0.001255388	40.0	27
TACCGTA	20	0.001255388	40.0	16
TATACGG	95	0.0	37.894733	2
CGCATGG	120	0.0	36.666664	2
TAGCACG	60	1.4551915E-11	36.666664	1
GTATACG	60	1.4551915E-11	36.666664	1
TACGGGT	55	2.1645974E-10	36.363636	4
GCGATAC	55	2.1645974E-10	36.363636	9
ATAACGG	55	2.1645974E-10	36.363636	2
TACAGCG	55	2.1645974E-10	36.363636	1
>>END_MODULE