##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	628939
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.750834341645216	31.0	31.0	34.0	30.0	34.0
2	31.902965152423366	33.0	31.0	34.0	30.0	34.0
3	31.867958577858904	33.0	31.0	34.0	30.0	34.0
4	35.627558475464234	37.0	35.0	37.0	33.0	37.0
5	35.42960287086665	37.0	35.0	37.0	33.0	37.0
6	35.424783643564794	37.0	35.0	37.0	33.0	37.0
7	35.748687869570816	37.0	35.0	37.0	35.0	37.0
8	35.54107314063844	37.0	35.0	37.0	35.0	37.0
9	37.30186711270886	39.0	37.0	39.0	34.0	39.0
10	36.75552637060192	39.0	37.0	39.0	32.0	39.0
11	36.713779873723844	39.0	37.0	39.0	32.0	39.0
12	36.75039550735445	39.0	37.0	39.0	32.0	39.0
13	35.13358370207604	39.0	35.0	39.0	30.0	39.0
14	36.905137064166794	39.0	36.0	41.0	30.0	41.0
15	37.5081828285414	40.0	36.0	41.0	32.0	41.0
16	37.69274921733268	40.0	36.0	41.0	33.0	41.0
17	37.72257404931162	39.0	37.0	41.0	33.0	41.0
18	37.75396501091521	39.0	37.0	41.0	33.0	41.0
19	37.72290317502969	39.0	36.0	41.0	33.0	41.0
20	37.69770518285557	39.0	36.0	41.0	33.0	41.0
21	37.56272070900358	39.0	36.0	41.0	32.0	41.0
22	37.537355769001444	39.0	36.0	41.0	32.0	41.0
23	37.45068440659587	39.0	36.0	41.0	32.0	41.0
24	37.3968556569079	39.0	36.0	41.0	32.0	41.0
25	37.194774055989534	39.0	35.0	41.0	31.0	41.0
26	37.1875778096127	39.0	35.0	41.0	32.0	41.0
27	37.16892576227583	39.0	35.0	41.0	32.0	41.0
28	37.00190002528067	39.0	35.0	41.0	31.0	41.0
29	36.909655785378234	39.0	35.0	40.0	31.0	41.0
30	36.78445763420618	39.0	35.0	40.0	31.0	41.0
31	36.742521929789696	39.0	35.0	40.0	31.0	41.0
32	36.62900376666099	39.0	35.0	40.0	30.0	41.0
33	36.51121173913528	39.0	35.0	40.0	30.0	41.0
34	36.396127446381925	39.0	35.0	40.0	30.0	41.0
35	36.28861622510291	39.0	35.0	40.0	30.0	41.0
36	36.1729611297757	39.0	35.0	40.0	30.0	41.0
37	36.103223047068155	38.0	35.0	40.0	30.0	41.0
38	35.945236342475184	38.0	35.0	40.0	29.0	41.0
39	35.819340190384125	38.0	35.0	40.0	28.0	41.0
40	35.731932667555995	38.0	35.0	40.0	28.0	41.0
41	35.63850389306435	38.0	34.0	40.0	28.0	41.0
42	35.571767691302334	38.0	34.0	40.0	28.0	41.0
43	35.46310055506178	38.0	34.0	40.0	27.0	41.0
44	35.33743812992993	38.0	34.0	40.0	27.0	41.0
45	35.23195254229743	38.0	34.0	40.0	27.0	41.0
46	34.17950866459228	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	14.0
10	29.0
11	16.0
12	16.0
13	15.0
14	14.0
15	25.0
16	50.0
17	95.0
18	172.0
19	353.0
20	564.0
21	920.0
22	1449.0
23	2092.0
24	2919.0
25	3972.0
26	5474.0
27	6966.0
28	8808.0
29	10861.0
30	13579.0
31	17293.0
32	21617.0
33	28684.0
34	41600.0
35	53118.0
36	63916.0
37	90593.0
38	129663.0
39	124044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.38193370104255	23.666842094384354	22.498843290048796	20.4523809145243
2	25.694860709862166	28.262518304636856	23.028624397596587	23.01399658790439
3	27.63256850028381	27.16193462323055	23.318318628674643	21.887178247810997
4	25.96070525122468	28.141679876744803	21.52768392483214	24.369930947198377
5	21.049259149138468	33.35522204856115	20.24504761193057	25.35047119036981
6	20.504691233967044	40.86215038342351	20.864503552808777	17.768654829800663
7	82.96623360930074	6.59984513601478	6.442119188029364	3.9918020666551124
8	82.41578277066615	6.3039499856106875	6.706373750077511	4.573893493645648
9	75.40222501705253	8.461869911072457	9.62891472781939	6.506990344055624
10	33.97181602667349	29.6979516296493	17.10165850742282	19.22857383625439
11	28.08062467107303	28.792140414253208	23.134199024070696	19.993035890603064
12	24.019976500105734	25.31199369096208	29.82960191687906	20.838427892053126
13	22.900631062789873	27.029648344275042	30.227891735128527	19.841828857806558
14	22.182596404420778	27.79951632829257	27.883785231954132	22.13410203533252
15	20.88453729216983	27.004685669039446	29.85376960245747	22.257007436333254
16	22.960732280873025	26.010312605832997	28.671937978086902	22.357017135207073
17	22.546224673616997	26.09664848260324	26.924073717800933	24.43305312597883
18	23.78370557399048	26.157544690343578	26.790674453325362	23.26807528234058
19	24.6435663872013	28.25043446184765	25.512649080435462	21.593350070515584
20	26.94410745716198	26.160883646903756	24.459605780528797	22.43540311540547
21	27.450356870857114	27.0988124444501	25.402622511881116	20.04820817281167
22	24.763927821299045	25.721413364412125	24.93771256035959	24.576946253929236
23	24.026654413226083	28.126734071189734	24.891126166448572	22.955485349135607
24	22.735273214095482	27.462440713646313	26.384275740572615	23.418010331685586
25	24.222698862687796	27.889668155417297	24.93453260173085	22.953100380164056
26	22.440173053348573	27.746888012986954	25.57418127990155	24.238757653762924
27	24.1296850727972	25.13693696845004	26.223051838095586	24.51032612065717
28	23.01399658790439	28.28763997780389	26.426251194471963	22.272112239819762
29	24.63498049890371	27.933074590699576	24.83468190078847	22.597263009608245
30	24.815284153153165	26.312090679700255	25.449208905792137	23.42341626135444
31	25.71727941819477	27.64449334514158	23.761445863589316	22.876781373074337
32	25.702651608502574	26.530712835426012	25.162058641617076	22.604576914454345
33	24.32477553467029	26.683668845468322	23.871313434212222	25.120242185649168
34	23.866543496269113	27.480884473692996	26.02621239897669	22.6263596310612
35	23.31974961005757	26.064848896315855	27.225215799942443	23.390185693684128
36	24.807652252444196	28.044214144773978	24.99431582395113	22.1538177788307
37	24.797476384832233	27.011045586296923	26.361698034308574	21.82977999456227
38	23.472228626305572	27.672794976937347	26.929161651606915	21.925814745150166
39	24.602226925027708	25.65972216701461	26.43483708276955	23.303213825188134
40	23.78640853882491	25.321215570985423	29.94360343371933	20.94877245647034
41	22.476265583784755	25.842887784029926	27.852462639461063	23.828383992724255
42	24.60492988986213	26.350250183245116	26.309387714865828	22.73543221202692
43	23.54234671406925	24.588871098787006	28.032130301984772	23.836651885158975
44	25.05107808547411	24.8640965181043	26.74536004286584	23.33946535355575
45	24.143994886626523	23.68449086477385	27.689966753532534	24.481547495067087
46	24.0555920367476	25.377977832508396	26.549156595472695	24.017273535271304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4498.0
1	3943.5
2	3389.0
3	3762.0
4	4135.0
5	2985.5
6	1836.0
7	1836.0
8	1799.5
9	1763.0
10	1752.0
11	1741.0
12	1811.5
13	1882.0
14	1850.5
15	1819.0
16	1772.5
17	1726.0
18	1724.0
19	1722.0
20	1722.0
21	1806.0
22	1890.0
23	2500.0
24	3110.0
25	3811.5
26	4513.0
27	5213.0
28	5913.0
29	7363.0
30	8813.0
31	8813.0
32	10644.0
33	12475.0
34	13544.0
35	14613.0
36	16371.5
37	18130.0
38	19786.0
39	21442.0
40	24073.5
41	26705.0
42	30197.5
43	33690.0
44	33690.0
45	38056.0
46	42422.0
47	45984.5
48	49547.0
49	52994.0
50	56441.0
51	54172.0
52	51903.0
53	47182.5
54	42462.0
55	41381.5
56	40301.0
57	40301.0
58	38269.0
59	36237.0
60	34360.0
61	32483.0
62	29925.5
63	27368.0
64	24135.5
65	20903.0
66	18872.0
67	16841.0
68	14929.0
69	13017.0
70	13017.0
71	10944.5
72	8872.0
73	7088.0
74	5304.0
75	4719.5
76	4135.0
77	3419.0
78	2703.0
79	1936.0
80	1169.0
81	1169.0
82	883.0
83	597.0
84	394.5
85	192.0
86	179.5
87	167.0
88	108.0
89	49.0
90	30.5
91	12.0
92	8.5
93	5.0
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	628939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.7411355847797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0029107494682	21.26927691518816
2	9.307942396792338	5.350416688830558
3	3.5875558477411698	3.0933128711369466
4	1.883919480871484	2.1658394092214044
5	1.21203468012033	1.7417626537396746
6	0.9071700020659623	1.564385761669362
7	0.6953397123104884	1.3989397064298221
8	0.5775231154734866	1.3278936132134287
9	0.498571877811288	1.28965697450392
>10	6.651434067995055	44.6321194896743
>50	0.6063632258795821	10.826114171068221
>100	0.06309304284014025	3.2640582577046127
>500	0.004466764094877186	0.9264212547555915
>1k	0.0016750365355789449	1.1498022328639896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4134	0.6572974485601942	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1811	0.2879452538322476	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC	1220	0.19397747635303264	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	910	0.14468811760758993	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	864	0.13737421276149198	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	736	0.11702247753756724	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	733	0.11654548374325649	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTATTGTCGT	692	0.1100265685543431	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	672	0.10684660992560487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.17995862873824E-4	0.0	0.0	0.10780059751422634	0.0
2	3.17995862873824E-4	0.0	0.0	0.5108603537067983	0.0
3	3.17995862873824E-4	0.0	0.0	0.7008628817739081	0.0
4	3.17995862873824E-4	0.0	0.0	0.9463556879125002	0.0
5	3.17995862873824E-4	0.0	0.0	1.6538964828067586	0.0
6	3.17995862873824E-4	0.0	0.0	2.461446976574835	0.0
7	3.17995862873824E-4	0.0	0.0	3.051806296000089	0.0
8	3.17995862873824E-4	0.0	0.0	4.2264830134559945	0.0
9	3.17995862873824E-4	0.0	0.0	4.768506961724428	0.0
10	3.17995862873824E-4	0.0	0.0	5.733783403477921	0.0
11	3.17995862873824E-4	0.0	0.0	6.717185609415221	0.0
12	3.17995862873824E-4	0.0	0.0	7.501999398987819	0.0
13	3.17995862873824E-4	0.0	0.0	7.8436859536457435	0.0
14	3.17995862873824E-4	0.0	0.0	7.991395031950634	0.0
15	3.17995862873824E-4	0.0	0.0	8.197933344887183	0.0
16	3.17995862873824E-4	0.0	0.0	8.698935826844894	0.0
17	3.17995862873824E-4	0.0	0.0	9.235871841307345	0.0
18	3.17995862873824E-4	0.0	0.0	9.97855117904916	0.0
19	3.17995862873824E-4	0.0	0.0	10.273969335658943	0.0
20	3.17995862873824E-4	0.0	0.0	10.594986159230068	0.0
21	3.17995862873824E-4	0.0	0.0	10.951936515305936	0.0
22	3.17995862873824E-4	0.0	0.0	11.294259061689607	0.0
23	3.17995862873824E-4	0.0	0.0	11.649460440519668	0.0
24	3.17995862873824E-4	0.0	0.0	11.949648535072559	0.0
25	3.17995862873824E-4	0.0	0.0	12.209292157109036	0.0
26	3.17995862873824E-4	0.0	0.0	12.460985882573668	0.0
27	3.17995862873824E-4	0.0	0.0	12.717608543912844	0.0
28	3.17995862873824E-4	0.0	0.0	12.989495006669964	0.0
29	3.17995862873824E-4	0.0	0.0	13.270921345313297	0.0
30	3.17995862873824E-4	0.0	0.0	13.643453498669983	0.0
31	3.17995862873824E-4	0.0	0.0	13.93092175870792	0.0
32	4.76993794310736E-4	0.0	0.0	14.220138995991663	0.0
33	4.76993794310736E-4	0.0	0.0	14.516988133984377	0.0
34	4.76993794310736E-4	0.0	0.0	14.797142489176215	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	20	0.0012553354	40.0	12
CTCAACG	40	1.925946E-8	40.0	1
CCGGATT	20	0.0012553354	40.0	26
TAAGTCG	20	0.0012553354	40.0	1
CGTTAAG	20	0.0012553354	40.0	11
GTCGTTA	20	0.0012553354	40.0	9
GCGATCG	20	0.0012553354	40.0	9
ACGTCGG	25	7.7906225E-5	40.0	2
CGGTATG	20	0.0012553354	40.0	10
CGCAATG	20	0.0012553354	40.0	23
CTAACGG	65	0.0	36.923077	2
CATACGA	65	0.0	36.923077	18
TAGCACG	60	1.4551915E-11	36.666664	1
CACGACC	280	0.0	36.428574	27
CGTATGG	55	2.1645974E-10	36.36364	2
TTAACGG	55	2.1645974E-10	36.36364	2
GCGCTAG	55	2.1645974E-10	36.36364	1
ACGGGCT	340	0.0	35.882355	5
TACGAGG	95	0.0	35.78947	2
CAATGCG	45	5.435868E-8	35.555557	1
>>END_MODULE