FastQCFastQC Report
Sat 18 Jun 2016
SRR3553818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590874
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36400.61603658309555No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.2939713035266402No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT16900.2860169850086482No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT9270.15688623970592716No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9110.15417838659341923No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT8360.1414853251285384No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6950.11762236957456242No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA6700.11339134908626883No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6340.10729867958312603No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGT6100.10323689991436413No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC6090.1030676590948324No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG200.001255273440.0000041
CGTAGTG200.001255273440.00000428
CCATACT200.001255273440.00000431
ACGAACA200.001255273440.00000420
TTTCGAC257.79005E-540.030
GTATGCG304.863494E-640.01
CTACGAA850.040.011
TCATCGG304.863494E-640.02
TACGAAT900.037.77777512
GCTACGA900.037.77777510
CGTTCAT750.037.33333217
AACGGGA3400.036.470594
TAGGGCG3800.036.315795
AATATAC1050.036.19047516
GCACTTA5050.036.03960436
TACGAAG503.4197E-936.01
TATGCGG950.035.7894742
CGAATAT950.035.78947414
CGAAAGG1400.035.7142872
TGCGGGC1800.035.5555534