##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553818_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590874 Sequences flagged as poor quality 0 Sequence length 46 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.777419551376436 31.0 31.0 34.0 30.0 34.0 2 31.92794572108436 33.0 31.0 34.0 30.0 34.0 3 31.904566794274245 33.0 31.0 34.0 30.0 34.0 4 35.65607388377217 37.0 35.0 37.0 33.0 37.0 5 35.461665261967866 37.0 35.0 37.0 33.0 37.0 6 35.461121998937166 37.0 35.0 37.0 33.0 37.0 7 35.756658780044475 37.0 35.0 37.0 35.0 37.0 8 35.526956677735015 37.0 35.0 37.0 33.0 37.0 9 37.28017479191841 39.0 37.0 39.0 34.0 39.0 10 36.8036366467301 39.0 37.0 39.0 32.0 39.0 11 36.79852726638843 39.0 37.0 39.0 32.0 39.0 12 36.8526826362304 39.0 37.0 39.0 33.0 39.0 13 35.23237779966626 39.0 35.0 39.0 30.0 39.0 14 37.01488134526143 40.0 36.0 41.0 31.0 41.0 15 37.615339310919076 40.0 36.0 41.0 32.0 41.0 16 37.80328293341728 40.0 37.0 41.0 33.0 41.0 17 37.83262929152408 39.0 37.0 41.0 33.0 41.0 18 37.85614022617343 39.0 37.0 41.0 33.0 41.0 19 37.84845838537488 40.0 37.0 41.0 33.0 41.0 20 37.810885231030646 40.0 37.0 41.0 33.0 41.0 21 37.67501700870236 39.0 36.0 41.0 33.0 41.0 22 37.66216655327532 39.0 36.0 41.0 33.0 41.0 23 37.57819941307284 39.0 36.0 41.0 33.0 41.0 24 37.5364680117927 39.0 36.0 41.0 32.0 41.0 25 37.31927111363844 39.0 36.0 41.0 32.0 41.0 26 37.30114880668298 39.0 36.0 41.0 32.0 41.0 27 37.252710391724804 39.0 36.0 41.0 32.0 41.0 28 37.11572179517122 39.0 35.0 41.0 31.0 41.0 29 37.0208893943548 39.0 35.0 41.0 31.0 41.0 30 36.9012953692327 39.0 35.0 41.0 31.0 41.0 31 36.82361891029221 39.0 35.0 41.0 31.0 41.0 32 36.66413990123106 39.0 35.0 40.0 30.0 41.0 33 36.52312506558082 39.0 35.0 40.0 30.0 41.0 34 36.47891597870274 39.0 35.0 40.0 30.0 41.0 35 36.334868347566484 39.0 35.0 40.0 30.0 41.0 36 36.17270348669936 39.0 35.0 40.0 30.0 41.0 37 36.094118204557994 38.0 35.0 40.0 30.0 41.0 38 35.94657236568202 38.0 35.0 40.0 29.0 41.0 39 35.89270639764146 38.0 35.0 40.0 29.0 41.0 40 35.797811377721814 38.0 35.0 40.0 28.0 41.0 41 35.634091193723194 38.0 34.0 40.0 28.0 41.0 42 35.57548817514394 38.0 34.0 40.0 28.0 41.0 43 35.505131381648205 38.0 34.0 40.0 28.0 41.0 44 35.44649620731323 38.0 34.0 40.0 28.0 41.0 45 35.37367526748511 38.0 34.0 40.0 27.0 41.0 46 34.31510101984518 37.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 16.0 10 14.0 11 21.0 12 19.0 13 15.0 14 20.0 15 15.0 16 36.0 17 82.0 18 146.0 19 286.0 20 489.0 21 877.0 22 1304.0 23 1902.0 24 2654.0 25 3601.0 26 4793.0 27 6303.0 28 7821.0 29 9639.0 30 11826.0 31 15379.0 32 19877.0 33 26720.0 34 38260.0 35 49713.0 36 61896.0 37 86455.0 38 121777.0 39 118910.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.95969360642032 23.07378561249945 22.199318297978927 20.767202483101304 2 24.92984968030409 28.104468973080554 22.89794440100597 24.067736945609386 3 28.129855096010314 26.961585718782683 23.5474906663688 21.361068518838195 4 25.578042019110676 28.83169677460846 20.99753923848401 24.59272196779686 5 21.430964977304807 33.04630090340749 20.25338735500293 25.269346764284773 6 20.05080609402343 40.787545229609016 20.646195297136106 18.515453379231445 7 82.04422601096003 6.658103081198361 7.116914942948919 4.180755964892684 8 80.91657443042001 6.594637773873956 7.241814667763347 5.246973127942675 9 73.68051395052075 8.671391870347993 10.837505119534791 6.810589059596463 10 32.82781100539201 29.548262404505866 17.46751422469088 20.15641236541124 11 27.09139342736353 29.13565328648747 23.297183494281352 20.47576979186764 12 23.500780200178042 24.904632798193862 30.282259838815044 21.31232716281305 13 22.670823221194368 26.97427878024757 30.30916912912059 20.045728869437475 14 21.499676750034695 28.35105284713831 27.6382105152706 22.5110598875564 15 20.391657104560363 26.988833490727295 30.707223536659257 21.91228586805309 16 22.140422492781877 26.799791495310338 28.373900357775096 22.685885654132694 17 21.564495983915354 27.185660563842713 26.61261114890823 24.637232303333708 18 23.805921397793778 25.510176450478443 27.893933393583065 22.789968758144717 19 24.012564438442034 28.174703913186228 25.60867460744592 22.204057040925814 20 25.707172764413393 25.94106357700626 24.854537515612467 23.497226142967875 21 26.62835054512468 27.66562752803474 25.006008049093374 20.7000138777472 22 24.342245554889875 25.77926935353392 24.39030994763689 25.48817514393932 23 23.107295294766736 27.43444456855438 25.743559540612722 23.714700596066166 24 22.73902727146566 26.805037960715822 26.834485863314345 23.621448904504174 25 23.568138046351674 27.69744480210671 25.528962181446467 23.205454970095147 26 22.62292806926688 27.565775444511015 25.49985276048701 24.311443725735096 27 23.510088445252286 26.79014476859703 25.710219099164966 23.98954768698572 28 22.40020715076311 27.825052380033643 26.291730555076036 23.48300991412721 29 23.48199446921002 28.223783750850433 24.256440459387278 24.037781320552266 30 25.420986538585215 26.53983759650958 24.63672458087511 23.4024512840301 31 25.181510778947796 28.010371077420903 22.925192172950577 23.88292597068072 32 24.833382413170998 27.950459827306666 23.897819162799514 23.31833859672282 33 23.316815429347034 28.80207963119041 23.06803142463537 24.813073514827188 34 23.648527435629255 27.709460900293465 24.90175570426182 23.74025595981546 35 24.919356749493122 27.260126524436686 24.11884767310797 23.70166905296222 36 24.825428094653006 29.242613484431534 23.782227683059332 22.149730737856125 37 24.465622112328518 29.285431411773068 24.315167023764797 21.93377945213362 38 23.04213757924701 29.30878664486845 25.825472097266083 21.823603678618454 39 24.913771802448576 26.466217840013268 24.278103284287344 24.341907073250812 40 25.932263054390614 25.892999184259253 27.537512227649213 20.637225533700924 41 22.412561730588923 26.629027508402807 26.93383022437948 24.02458053662879 42 23.261981403818748 27.512803067997577 26.185785802049168 23.039429726134504 43 22.42559327369287 26.55134597223774 27.757017570581883 23.26604318348751 44 23.259612032345306 26.278529771152563 25.847811885444273 24.61404631105786 45 24.755362395366866 24.41248049499555 26.08610295934497 24.74605415029262 46 25.27510095214885 25.73103571996737 25.58531937435054 23.40854395353324 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3928.0 1 3452.5 2 2977.0 3 3369.5 4 3762.0 5 2723.5 6 1685.0 7 1685.0 8 1632.5 9 1580.0 10 1609.0 11 1638.0 12 1698.5 13 1759.0 14 1730.0 15 1701.0 16 1570.5 17 1440.0 18 1466.0 19 1492.0 20 1492.0 21 1568.5 22 1645.0 23 2092.0 24 2539.0 25 3377.5 26 4216.0 27 5483.5 28 6751.0 29 7024.5 30 7298.0 31 7298.0 32 8764.5 33 10231.0 34 11697.0 35 13163.0 36 14393.5 37 15624.0 38 17721.0 39 19818.0 40 22187.0 41 24556.0 42 28157.0 43 31758.0 44 31758.0 45 36540.5 46 41323.0 47 47203.5 48 53084.0 49 54766.0 50 56448.0 51 53668.0 52 50888.0 53 46200.5 54 41513.0 55 38495.5 56 35478.0 57 35478.0 58 34022.0 59 32566.0 60 31063.5 61 29561.0 62 27049.5 63 24538.0 64 22049.0 65 19560.0 66 17190.0 67 14820.0 68 13089.0 69 11358.0 70 11358.0 71 9560.5 72 7763.0 73 6203.5 74 4644.0 75 4087.5 76 3531.0 77 2939.0 78 2347.0 79 1731.5 80 1116.0 81 1116.0 82 802.0 83 488.0 84 326.0 85 164.0 86 129.0 87 94.0 88 55.0 89 16.0 90 12.0 91 8.0 92 5.0 93 2.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 590874.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.46053327810343 #Duplication Level Percentage of deduplicated Percentage of total 1 74.47304550583146 22.684886809520904 2 9.2791118090706 5.6529338810287495 3 3.430063945723559 3.1344473089420575 4 1.8396590293931876 2.2414798034077856 5 1.1465118837840256 1.746168169487218 6 0.8576580025545351 1.5674832076826481 7 0.680883094472463 1.451804351037355 8 0.5889517684839896 1.4351827954483545 9 0.4956809926167662 1.3588836633833712 >10 6.831445208597166 47.65647344753789 >50 0.32257222722698925 6.050423322619784 >100 0.04768459011181557 2.707969000298114 >500 0.00504895660007459 1.104240277650433 >1k 0.0016829855333581967 1.2076239619553715 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3640 0.61603658309555 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1737 0.2939713035266402 No Hit CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT 1690 0.2860169850086482 No Hit GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT 927 0.15688623970592716 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 911 0.15417838659341923 No Hit CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT 836 0.1414853251285384 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 695 0.11762236957456242 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA 670 0.11339134908626883 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 634 0.10729867958312603 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGT 610 0.10323689991436413 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC 609 0.1030676590948324 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15925561117937156 0.0 2 0.0 0.0 0.0 0.7719073778842866 0.0 3 0.0 0.0 0.0 1.0151064355514035 0.0 4 0.0 0.0 0.0 1.297569363349885 0.0 5 0.0 0.0 0.0 2.101801737764735 0.0 6 0.0 0.0 0.0 3.140940369689646 0.0 7 0.0 0.0 0.0 3.9196173803552026 0.0 8 0.0 0.0 0.0 5.338870892948412 0.0 9 0.0 0.0 0.0 5.971662317177604 0.0 10 0.0 0.0 0.0 7.001661944847802 0.0 11 0.0 0.0 0.0 8.030646127600809 0.0 12 0.0 0.0 0.0 8.908329017692436 0.0 13 0.0 0.0 0.0 9.331431066521796 0.0 14 0.0 0.0 0.0 9.50930316784966 0.0 15 0.0 0.0 0.0 9.77314960549965 0.0 16 0.0 0.0 0.0 10.341291036667716 0.0 17 0.0 0.0 0.0 10.939557333712433 0.0 18 0.0 0.0 0.0 11.864458412453416 0.0 19 0.0 0.0 0.0 12.17620000203089 0.0 20 0.0 0.0 0.0 12.562576793021863 0.0 21 0.0 0.0 0.0 12.973831984484002 0.0 22 0.0 0.0 0.0 13.367316889895308 0.0 23 0.0 0.0 0.0 13.80886618805363 0.0 24 0.0 0.0 0.0 14.136685655486618 0.0 25 0.0 0.0 0.0 14.439626722448441 0.0 26 0.0 0.0 0.0 14.697042008956224 0.0 27 0.0 0.0 0.0 15.030954145892355 0.0 28 0.0 0.0 0.0 15.320863669750235 0.0 29 0.0 0.0 0.0 15.640728818665231 0.0 30 0.0 0.0 0.0 16.055368826518006 0.0 31 0.0 0.0 0.0 16.364741044622033 0.0 32 0.0 0.0 0.0 16.679021246492486 0.0 33 0.0 0.0 0.0 16.99008587279183 0.0 34 0.0 0.0 0.0 17.28507262123566 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTACG 20 0.0012552734 40.000004 1 CGTAGTG 20 0.0012552734 40.000004 28 CCATACT 20 0.0012552734 40.000004 31 ACGAACA 20 0.0012552734 40.000004 20 TTTCGAC 25 7.79005E-5 40.0 30 GTATGCG 30 4.863494E-6 40.0 1 CTACGAA 85 0.0 40.0 11 TCATCGG 30 4.863494E-6 40.0 2 TACGAAT 90 0.0 37.777775 12 GCTACGA 90 0.0 37.777775 10 CGTTCAT 75 0.0 37.333332 17 AACGGGA 340 0.0 36.47059 4 TAGGGCG 380 0.0 36.31579 5 AATATAC 105 0.0 36.190475 16 GCACTTA 505 0.0 36.039604 36 TACGAAG 50 3.4197E-9 36.0 1 TATGCGG 95 0.0 35.789474 2 CGAATAT 95 0.0 35.789474 14 CGAAAGG 140 0.0 35.714287 2 TGCGGGC 180 0.0 35.555553 4 >>END_MODULE