FastQCFastQC Report
Sat 18 Jun 2016
SRR3553815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659000
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41360.6276176024279211No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.5273141122913505No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT7210.10940819423368742No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7050.10698027314112292No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT6700.10166919575113809No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT6660.10106221547799697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGT257.7910314E-540.034
CAAACGC257.7910314E-540.034
GTCGATA200.001255379140.030
ACCGAAT200.001255379140.025
CGTTCGA257.7910314E-540.014
GCCGATA401.9261279E-840.09
GTCCCGA200.001255379140.038
TTCATCG200.001255379140.014
GACGATT200.001255379140.028
CGGTATG451.2205419E-940.06
CACCGAA304.8643487E-640.024
CCCGGAT304.8643487E-640.019
GTGCGAT200.001255379140.017
TGTCGAT200.001255379140.029
GCCCGTT200.001255379140.033
ATACAAC200.001255379140.012
TATCCGG353.0537376E-740.02
ACGGTCT401.9261279E-840.030
CGGGTAT1550.038.7096756
CATATCA1000.038.029