##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659000
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810098634294384	31.0	31.0	34.0	30.0	34.0
2	31.962787556904402	33.0	31.0	34.0	30.0	34.0
3	31.908945371775417	33.0	31.0	34.0	30.0	34.0
4	35.67222761760243	37.0	35.0	37.0	33.0	37.0
5	35.481858877086495	37.0	35.0	37.0	33.0	37.0
6	35.48844157814871	37.0	35.0	37.0	33.0	37.0
7	35.78157207890744	37.0	35.0	37.0	35.0	37.0
8	35.58436418816388	37.0	35.0	37.0	35.0	37.0
9	37.34601972685888	39.0	37.0	39.0	34.0	39.0
10	36.82068285280728	39.0	37.0	39.0	32.0	39.0
11	36.79902124430956	39.0	37.0	39.0	32.0	39.0
12	36.81835963581184	39.0	37.0	39.0	33.0	39.0
13	35.15269802731411	39.0	35.0	39.0	30.0	39.0
14	36.968327769347496	40.0	36.0	41.0	30.0	41.0
15	37.594280728376326	40.0	36.0	41.0	32.0	41.0
16	37.78111836115326	40.0	37.0	41.0	33.0	41.0
17	37.82184522003035	39.0	37.0	41.0	33.0	41.0
18	37.840298937784524	39.0	37.0	41.0	33.0	41.0
19	37.7917875569044	40.0	37.0	41.0	33.0	41.0
20	37.749743550834594	40.0	36.0	41.0	33.0	41.0
21	37.622039453717754	39.0	36.0	41.0	33.0	41.0
22	37.602253414264034	39.0	36.0	41.0	33.0	41.0
23	37.501151745068285	39.0	36.0	41.0	32.0	41.0
24	37.430367223065254	39.0	36.0	41.0	32.0	41.0
25	37.215810318664644	39.0	35.0	41.0	32.0	41.0
26	37.205723823975724	39.0	35.0	41.0	32.0	41.0
27	37.1721502276176	39.0	35.0	41.0	32.0	41.0
28	37.03640819423369	39.0	35.0	41.0	31.0	41.0
29	36.95081790591806	39.0	35.0	41.0	31.0	41.0
30	36.78790440060698	39.0	35.0	40.0	31.0	41.0
31	36.71649165402125	39.0	35.0	40.0	31.0	41.0
32	36.5782564491654	39.0	35.0	40.0	30.0	41.0
33	36.46812291350531	39.0	35.0	40.0	30.0	41.0
34	36.33046737481032	39.0	35.0	40.0	30.0	41.0
35	36.24894840667678	39.0	35.0	40.0	30.0	41.0
36	36.08991502276176	38.0	35.0	40.0	29.0	41.0
37	36.05774658573596	38.0	35.0	40.0	29.0	41.0
38	35.927405159332324	38.0	35.0	40.0	29.0	41.0
39	35.81020485584219	38.0	35.0	40.0	29.0	41.0
40	35.68377086494689	38.0	35.0	40.0	28.0	41.0
41	35.55181335356601	38.0	35.0	40.0	27.0	41.0
42	35.44322306525038	38.0	34.0	40.0	27.0	41.0
43	35.36289529590288	38.0	34.0	40.0	27.0	41.0
44	35.22260091047041	38.0	34.0	40.0	26.0	41.0
45	35.14278603945372	38.0	34.0	40.0	26.0	41.0
46	34.10184522003035	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	24.0
10	32.0
11	30.0
12	21.0
13	20.0
14	20.0
15	29.0
16	48.0
17	106.0
18	178.0
19	342.0
20	614.0
21	1000.0
22	1604.0
23	2261.0
24	3246.0
25	4248.0
26	5764.0
27	7415.0
28	9349.0
29	11193.0
30	13674.0
31	17187.0
32	22347.0
33	29633.0
34	43708.0
35	55701.0
36	67970.0
37	93982.0
38	134642.0
39	132600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.47678300455235	22.85720789074355	21.721699544764796	21.944309559939303
2	25.943854324734446	27.171623672230655	23.129438543247343	23.755083459787556
3	28.307738998482552	27.116388467374808	23.335660091047043	21.2402124430956
4	25.184066767830043	28.45204855842185	22.047040971168435	24.316843702579664
5	21.330955993930196	32.807283763277695	20.95386949924127	24.907890743550833
6	20.385735963581183	40.428528072837636	21.085128983308042	18.10060698027314
7	82.19241274658575	6.512746585735964	7.394081942336873	3.9007587253414266
8	81.79514415781487	6.096054628224583	7.48649468892261	4.622306525037937
9	75.18679817905918	8.154779969650987	10.559939301972685	6.098482549317148
10	34.252200303490135	28.662822458270103	17.583459787556905	19.501517450682854
11	27.82018209408194	28.67101669195751	23.558118361153262	19.950682852807283
12	23.221396054628222	24.44992412746586	30.30485584218513	22.023823975720788
13	23.142185128983307	26.107283763277696	30.58952959028832	20.161001517450682
14	21.91502276176024	27.395599393019726	28.026403641881636	22.66297420333839
15	21.04339908952959	25.871623672230655	30.832625189681334	22.25235204855842
16	22.89226100151745	25.69059180576631	28.67177541729894	22.7453717754173
17	22.020182094081942	25.42291350531108	28.195447647951443	24.36145675265554
18	23.29620637329287	24.893626707132018	28.32928679817906	23.480880121396055
19	24.960242792109256	27.469195751138088	25.7701062215478	21.800455235204854
20	26.03444613050076	25.923065250379363	25.50333839150228	22.5391502276176
21	26.683915022761763	26.557511380880122	25.897268588770867	20.86130500758725
22	24.636115326251897	25.16919575113809	25.23080424886191	24.9638846737481
23	23.57738998482549	27.057359635811835	25.352959028831563	24.012291350531108
24	22.652959028831564	26.598937784522004	27.227921092564493	23.520182094081942
25	24.07101669195751	27.24400606980273	25.0804248861912	23.604552352048557
26	22.554931714719274	26.9453717754173	25.935053110773897	24.56464339908953
27	22.824582701062216	25.923065250379363	26.82443095599393	24.427921092564493
28	22.223065250379364	27.606676783004552	27.144613050075872	23.025644916540212
29	23.42503793626707	27.28103186646434	25.506373292867984	23.787556904400606
30	23.910622154779972	26.841578148710166	26.03429438543247	23.21350531107739
31	24.194992412746586	28.327465857359634	24.329742033383912	23.147799696509864
32	24.303186646433993	27.822913505311075	24.38679817905918	23.487101669195752
33	23.444006069802732	27.21380880121396	24.585432473444612	24.756752655538694
34	23.271775417298937	28.82245827010622	25.00242792109256	22.903338391502277
35	25.012594840667678	26.027921092564494	25.220485584218512	23.738998482549317
36	23.445220030349017	29.6154779969651	23.61836115326252	23.32094081942337
37	24.232018209408192	28.221699544764796	24.42245827010622	23.12382397572079
38	23.896509863429436	28.154779969650985	24.602124430955993	23.346585735963583
39	24.427162367223065	25.66540212443096	24.190440060698027	25.71699544764795
40	24.501062215477997	25.113657056145676	27.4040971168437	22.981183611532625
41	22.031714719271626	25.427769347496206	26.661911987860393	25.878603945371776
42	22.907435508345976	26.463732928679818	26.116236722306525	24.512594840667678
43	22.98649468892261	24.508042488619118	28.115174506828527	24.390288315629743
44	23.46722306525038	26.139757207890746	26.36176024279211	24.031259484066766
45	23.76934749620637	24.659635811836115	27.17996965098634	24.391047040971166
46	24.0247344461305	25.79423368740516	25.899544764795145	24.281487101669196
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4418.0
1	3706.0
2	2994.0
3	4739.5
4	6485.0
5	4553.5
6	2622.0
7	2622.0
8	2467.5
9	2313.0
10	2321.5
11	2330.0
12	2370.0
13	2410.0
14	2296.5
15	2183.0
16	2095.5
17	2008.0
18	1984.5
19	1961.0
20	1961.0
21	2218.0
22	2475.0
23	2926.5
24	3378.0
25	4114.0
26	4850.0
27	5207.0
28	5564.0
29	7192.5
30	8821.0
31	8821.0
32	10569.0
33	12317.0
34	13246.5
35	14176.0
36	15611.5
37	17047.0
38	19409.0
39	21771.0
40	25366.5
41	28962.0
42	32215.5
43	35469.0
44	35469.0
45	39616.5
46	43764.0
47	48590.5
48	53417.0
49	55588.5
50	57760.0
51	54641.5
52	51523.0
53	46840.0
54	42157.0
55	39754.0
56	37351.0
57	37351.0
58	36194.0
59	35037.0
60	33758.0
61	32479.0
62	31578.0
63	30677.0
64	27985.5
65	25294.0
66	22499.5
67	19705.0
68	17475.5
69	15246.0
70	15246.0
71	13130.0
72	11014.0
73	9176.0
74	7338.0
75	6222.5
76	5107.0
77	3994.0
78	2881.0
79	2349.0
80	1817.0
81	1817.0
82	1485.5
83	1154.0
84	807.5
85	461.0
86	295.5
87	130.0
88	99.0
89	68.0
90	43.5
91	19.0
92	24.0
93	29.0
94	29.0
95	21.0
96	13.0
97	8.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	659000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.26293325980426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89504875870762	23.84071026332165
2	9.134252457513545	5.89395554829925
3	3.488042248336915	3.3760342279641447
4	1.9269939590023406	2.4868190996535393
5	1.303267249669914	2.102361214789454
6	0.9828280671864065	1.9025349802498468
7	0.7927898100137653	1.7904407310668904
8	0.6998621701916149	1.8063685190363072
9	0.6015256763587142	1.7466284475377833
>10	6.952004508176795	47.254220395909705
>50	0.18324189598170412	3.7008930576478702
>100	0.035892742510770057	2.2578053162668277
>500	0.0033059104944130314	0.6815469820260169
>1k	9.445458555465804E-4	1.159681216230721
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4136	0.6276176024279211	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3475	0.5273141122913505	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	721	0.10940819423368742	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	705	0.10698027314112292	No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	670	0.10166919575113809	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	666	0.10106221547799697	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11244309559939301	0.0
2	0.0	0.0	0.0	0.4543247344461305	0.0
3	0.0	0.0	0.0	0.65402124430956	0.0
4	0.0	0.0	0.0	0.8908952959028832	0.0
5	0.0	0.0	0.0	1.4918057663125948	0.0
6	0.0	0.0	0.0	2.3626707132018208	0.0
7	0.0	0.0	0.0	3.001820940819423	0.0
8	0.0	0.0	0.0	4.284370257966616	0.0
9	0.0	0.0	0.0	4.915022761760243	0.0
10	0.0	0.0	0.0	5.944916540212443	0.0
11	0.0	0.0	0.0	6.92185128983308	0.0
12	0.0	0.0	0.0	7.739453717754173	0.0
13	0.0	0.0	0.0	8.120182094081942	0.0
14	0.0	0.0	0.0	8.303186646433991	0.0
15	0.0	0.0	0.0	8.539453717754173	0.0
16	0.0	0.0	0.0	9.01380880121396	0.0
17	0.0	0.0	0.0	9.535204855842185	0.0
18	0.0	0.0	0.0	10.257056145675266	0.0
19	0.0	0.0	0.0	10.540971168437025	0.0
20	0.0	0.0	0.0	10.876934749620638	0.0
21	0.0	0.0	0.0	11.225493171471927	0.0
22	0.0	0.0	0.0	11.577389984825492	0.0
23	1.5174506828528073E-4	0.0	0.0	11.95751138088012	0.0
24	1.5174506828528073E-4	0.0	0.0	12.259787556904401	0.0
25	1.5174506828528073E-4	0.0	0.0	12.523520485584218	0.0
26	1.5174506828528073E-4	0.0	0.0	12.7649468892261	0.0
27	1.5174506828528073E-4	0.0	0.0	13.039150227617602	0.0
28	1.5174506828528073E-4	0.0	0.0	13.302276176024279	0.0
29	1.5174506828528073E-4	0.0	0.0	13.57784522003035	0.0
30	1.5174506828528073E-4	0.0	0.0	13.933383915022763	0.0
31	1.5174506828528073E-4	0.0	0.0	14.202883156297421	0.0
32	1.5174506828528073E-4	0.0	0.0	14.49256449165402	0.0
33	1.5174506828528073E-4	0.0	0.0	14.787556904400606	0.0
34	1.5174506828528073E-4	0.0	0.0	15.06236722306525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGT	25	7.7910314E-5	40.0	34
CAAACGC	25	7.7910314E-5	40.0	34
GTCGATA	20	0.0012553791	40.0	30
ACCGAAT	20	0.0012553791	40.0	25
CGTTCGA	25	7.7910314E-5	40.0	14
GCCGATA	40	1.9261279E-8	40.0	9
GTCCCGA	20	0.0012553791	40.0	38
TTCATCG	20	0.0012553791	40.0	14
GACGATT	20	0.0012553791	40.0	28
CGGTATG	45	1.2205419E-9	40.0	6
CACCGAA	30	4.8643487E-6	40.0	24
CCCGGAT	30	4.8643487E-6	40.0	19
GTGCGAT	20	0.0012553791	40.0	17
TGTCGAT	20	0.0012553791	40.0	29
GCCCGTT	20	0.0012553791	40.0	33
ATACAAC	20	0.0012553791	40.0	12
TATCCGG	35	3.0537376E-7	40.0	2
ACGGTCT	40	1.9261279E-8	40.0	30
CGGGTAT	155	0.0	38.709675	6
CATATCA	100	0.0	38.0	29
>>END_MODULE