FastQCFastQC Report
Sat 18 Jun 2016
SRR3553813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959493
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53930.562067675324364No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGT45890.4782734214840546No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.4095913154134528No Hit
TGAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC30740.32037753271779995No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT27130.282753495856666No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT20900.21782337130130183No Hit
TGAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG20790.2166769325049792No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC17980.18739063234437353No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA15920.16592096034051318No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTC15250.15893810585382073No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT14880.15508190262982638No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC12970.13517555625731506No Hit
TGAATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC12800.1334037872084528No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG11790.12287739462403582No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC11630.12120984728393015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT200.001255665240.032
ACGTTAG257.7936915E-540.01
TCGGAGT200.001255665240.023
CCGTACG200.001255665240.01
GTTCGGA200.001255665240.07
TACGACG353.0556657E-740.01
TCTCGCA200.001255665240.015
CGTACGG451.2205419E-940.02
CGGTCGA257.7936915E-540.029
TATCGTC200.001255665240.013
TATCGAG200.001255665240.01
CCGCAAC200.001255665240.039
CGTTTTT18400.037.51
CGACGGT601.4551915E-1136.66666428
ACGCATT2250.036.44444317
CGACAGG1650.036.3636362
GTTAACG552.1645974E-1036.3636361
AATCCGG503.423338E-936.02
TCACCGC2300.035.65217624
CTATGCG455.440961E-835.5555571
Produced by FastQC (version 0.11.5)