##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553813_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	959493
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53747760536033	31.0	31.0	34.0	30.0	34.0
2	31.693250497919212	31.0	31.0	34.0	30.0	34.0
3	31.621667901693915	31.0	31.0	34.0	30.0	34.0
4	35.46750836118658	37.0	35.0	37.0	33.0	37.0
5	33.576292896352555	37.0	35.0	37.0	30.0	37.0
6	34.39142026049174	37.0	35.0	37.0	30.0	37.0
7	35.29999176648501	37.0	35.0	37.0	32.0	37.0
8	35.4091181488557	37.0	35.0	37.0	33.0	37.0
9	37.08951811008522	39.0	37.0	39.0	33.0	39.0
10	36.53085431576885	38.0	35.0	39.0	32.0	39.0
11	36.31402834622035	38.0	35.0	39.0	32.0	39.0
12	36.586692138452285	39.0	35.0	39.0	32.0	39.0
13	33.06338660104868	38.0	35.0	39.0	10.0	39.0
14	35.258303082982366	39.0	35.0	40.0	18.0	41.0
15	36.63255073252228	39.0	35.0	40.0	30.0	41.0
16	37.20508330962289	39.0	36.0	40.0	32.0	41.0
17	37.41259706949399	39.0	36.0	40.0	32.0	41.0
18	37.48733133019209	39.0	36.0	40.0	32.0	41.0
19	37.48125207792032	39.0	36.0	40.0	32.0	41.0
20	37.457289422643	39.0	36.0	40.0	32.0	41.0
21	37.337220803069954	39.0	36.0	40.0	32.0	41.0
22	37.36263422453317	39.0	36.0	40.0	32.0	41.0
23	37.258781460625556	39.0	36.0	40.0	32.0	41.0
24	37.24188086833359	39.0	36.0	40.0	32.0	41.0
25	37.04322178483845	39.0	35.0	40.0	31.0	41.0
26	37.01291098528077	39.0	35.0	40.0	31.0	41.0
27	36.58028041892958	39.0	35.0	40.0	31.0	41.0
28	36.612734016819296	39.0	35.0	40.0	30.0	41.0
29	36.658554048857056	39.0	35.0	40.0	30.0	41.0
30	36.18453287309027	39.0	35.0	40.0	30.0	41.0
31	36.26371218966683	38.0	35.0	40.0	30.0	41.0
32	36.25159537380679	38.0	35.0	40.0	30.0	41.0
33	36.19143964572957	38.0	35.0	40.0	30.0	41.0
34	36.2002797310663	38.0	35.0	40.0	30.0	41.0
35	36.15567179750139	38.0	35.0	40.0	30.0	41.0
36	36.03700079104277	38.0	35.0	40.0	29.0	41.0
37	36.04707486141118	38.0	35.0	40.0	30.0	41.0
38	35.9175376995976	38.0	35.0	40.0	29.0	41.0
39	35.83870127244284	38.0	35.0	40.0	29.0	41.0
40	35.73985115055555	38.0	35.0	40.0	28.0	41.0
41	35.55648868725462	38.0	34.0	40.0	27.0	41.0
42	35.48629536640705	38.0	34.0	40.0	27.0	41.0
43	35.338968601125806	38.0	34.0	40.0	27.0	41.0
44	35.259130603349895	38.0	34.0	40.0	26.0	41.0
45	35.23164421209952	38.0	34.0	40.0	26.0	41.0
46	33.9564217769176	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	15.0
10	19.0
11	21.0
12	11.0
13	21.0
14	18.0
15	36.0
16	61.0
17	144.0
18	242.0
19	531.0
20	1045.0
21	1678.0
22	2545.0
23	3499.0
24	4753.0
25	6590.0
26	8726.0
27	11254.0
28	14801.0
29	18697.0
30	23950.0
31	30783.0
32	39939.0
33	52263.0
34	72110.0
35	90341.0
36	109015.0
37	144926.0
38	176957.0
39	144497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.023571823869485	23.887094538469796	23.45592932934373	21.633404308316997
2	27.963935119901866	26.516191363563884	22.204539272303187	23.315334244231067
3	25.866264787757704	28.762064965559937	23.82862615985734	21.543044086825024
4	24.443638463230062	29.64815793340858	21.97702328208752	23.93118032127384
5	19.345529357692033	35.00692553254688	21.873426903583454	23.77411820617764
6	20.86362276744072	38.71284105251419	21.8118318737083	18.611704306336783
7	79.20933242868891	7.230068379863115	8.443834400042523	5.116764791405461
8	78.56440849490303	5.617445880272185	9.718882784970813	6.099262839853965
9	70.64543462015878	9.459787617001895	12.513066796735359	7.381710966103975
10	31.563857162063712	26.740684924225604	20.554501179268634	21.140956734442042
11	25.53025399872641	27.984154131400647	25.66595066352751	20.819641206345434
12	22.14179780363171	22.89177721984423	31.95760677774616	23.0088181987779
13	21.113337981621545	29.272021786506	30.10329413554867	19.511346096323788
14	20.371800523818308	27.254706391813176	29.570095873549885	22.80339721081863
15	18.679552638737334	27.004261625671056	32.12071375195025	22.19547198364136
16	20.09905231200228	26.7145252753277	31.069325154013633	22.117097258656397
17	19.506760341138495	27.461899148821306	27.671384783422077	25.35995572661812
18	21.035588586889116	25.896384861588363	29.739768815405636	23.328257736116885
19	22.247791281437177	29.114959671409796	26.55589983460015	22.08134921255288
20	23.1596270113487	26.73443162170021	26.03520817765216	24.07073318929893
21	23.48135942628034	28.681293141273567	27.46533846521027	20.37200896723582
22	21.97785705575757	26.93724706694056	25.3004451309181	25.78445074638377
23	20.565236015270568	27.70900882028321	26.735682282205293	24.99007288224093
24	21.02881417581994	26.31514768737239	28.482750786092236	24.17328735071543
25	21.89760634001499	27.13818652142329	26.37351184427609	24.590695294285627
26	19.97648758250451	27.989469438547232	27.434905726253344	24.599137252694913
27	22.29521215892143	26.36871764567329	26.38435090198678	24.951719293418503
28	20.822142527355595	27.221355445011064	27.2882657820328	24.668236245600543
29	21.436529500475775	25.73223567029671	27.912970704319882	24.918264124907633
30	22.551493340753918	25.824263439128792	26.848762836206202	24.775480383911088
31	20.19014208545555	27.234695823731908	26.231353433532085	26.34380865728046
32	20.647571165188282	25.081266877402964	28.68525356620632	25.585908391202437
33	20.447778149501872	25.42884627610624	26.86887762599623	27.254497948395663
34	19.47695293243411	25.19611920045274	29.326946627020728	25.999981240092424
35	19.72833569395504	23.84436363787959	28.779261547504774	27.6480391206606
36	19.541570391863203	26.58685368210086	25.982888879856343	27.888687046179594
37	19.749596922541386	24.561408994125024	28.391139904095187	27.297854179238414
38	19.41066792566491	25.600290987010847	27.882850630489227	27.106190456835016
39	20.691656947992325	24.016016792201714	26.737037164419124	28.555289095386833
40	21.63486341223959	23.435293431009917	29.114125897739747	25.81571725901075
41	18.98492224539418	24.303772930078697	27.578419019211186	29.132885805315933
42	20.928761335413597	24.246763655388836	28.159767710655526	26.664707298542044
43	20.914795626440213	23.11116391677688	30.361659751556292	25.612380705226613
44	21.732519153344526	24.0379033510406	28.112763720006296	26.116813775608577
45	21.36138564846226	22.548262467782465	29.489532492681032	26.600819391074243
46	21.915428252212365	24.174954898055535	26.884406660600963	27.025210189131133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5771.0
1	4523.0
2	3275.0
3	5557.5
4	7840.0
5	5454.0
6	3068.0
7	3068.0
8	2936.5
9	2805.0
10	2819.0
11	2833.0
12	2893.0
13	2953.0
14	2852.0
15	2751.0
16	2656.0
17	2561.0
18	2681.5
19	2802.0
20	2802.0
21	2765.5
22	2729.0
23	3278.0
24	3827.0
25	4645.5
26	5464.0
27	6338.5
28	7213.0
29	9125.5
30	11038.0
31	11038.0
32	13558.0
33	16078.0
34	18196.0
35	20314.0
36	23236.5
37	26159.0
38	29848.5
39	33538.0
40	39039.5
41	44541.0
42	56370.5
43	68200.0
44	68200.0
45	77078.0
46	85956.0
47	92202.5
48	98449.0
49	100320.0
50	102191.0
51	91015.5
52	79840.0
53	70631.0
54	61422.0
55	57118.0
56	52814.0
57	52814.0
58	49536.0
59	46258.0
60	43268.5
61	40279.0
62	36950.0
63	33621.0
64	29429.5
65	25238.0
66	21906.5
67	18575.0
68	16357.0
69	14139.0
70	14139.0
71	12221.5
72	10304.0
73	8077.0
74	5850.0
75	4838.0
76	3826.0
77	3201.0
78	2576.0
79	1896.5
80	1217.0
81	1217.0
82	983.0
83	749.0
84	520.5
85	292.0
86	179.5
87	67.0
88	52.0
89	37.0
90	29.5
91	22.0
92	13.0
93	4.0
94	4.0
95	5.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	959493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.2469258262932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6715309750537	28.930257530424598
2	9.109932191184669	6.786339372152321
3	3.428588508605274	3.8311314560670553
4	1.8136059742117638	2.7020498879835113
5	1.1368336816016964	2.11717799077251
6	0.7984435682065292	1.784374101688168
7	0.5558638355419965	1.4492953336366108
8	0.43143788027855323	1.285578778031075
9	0.3656862301201496	1.2258619100083799
>10	4.394123547859107	37.27807792590003
>50	0.2516486100786509	5.74200632119251
>100	0.034389868738973026	2.301685659374183
>500	0.003821096526374325	0.9887458470235977
>1k	0.003821096526374325	3.0291651247956137
>5k	2.7293546616959465E-4	0.5482527609498522
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5393	0.562067675324364	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGT	4589	0.4782734214840546	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3930	0.4095913154134528	No Hit
TGAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	3074	0.32037753271779995	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	2713	0.282753495856666	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2090	0.21782337130130183	No Hit
TGAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG	2079	0.2166769325049792	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC	1798	0.18739063234437353	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	1592	0.16592096034051318	No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTC	1525	0.15893810585382073	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1488	0.15508190262982638	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	1297	0.13517555625731506	No Hit
TGAATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC	1280	0.1334037872084528	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG	1179	0.12287739462403582	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC	1163	0.12120984728393015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0422170875660374E-4	0.0	0.0	0.2578445074638377	0.0
2	1.0422170875660374E-4	0.0	0.0	0.9303871940702017	0.0
3	1.0422170875660374E-4	0.0	0.0	1.4730696315658374	0.0
4	1.0422170875660374E-4	0.0	0.0	1.9088205958771975	0.0
5	1.0422170875660374E-4	0.0	0.0	3.2778769621039445	0.0
6	1.0422170875660374E-4	0.0	0.0	5.187427109942439	0.0
7	1.0422170875660374E-4	0.0	0.0	6.490511134526255	0.0
8	1.0422170875660374E-4	0.0	0.0	8.828620948771903	0.0
9	1.0422170875660374E-4	0.0	0.0	9.786001565410066	0.0
10	1.0422170875660374E-4	0.0	0.0	11.238852185477121	0.0
11	1.0422170875660374E-4	0.0	0.0	13.092331054004562	0.0
12	1.0422170875660374E-4	0.0	0.0	14.232099660966782	0.0
13	1.0422170875660374E-4	0.0	0.0	15.116629303184077	0.0
14	1.0422170875660374E-4	0.0	0.0	15.406469875236192	0.0
15	1.0422170875660374E-4	0.0	0.0	15.801053264588694	0.0
16	1.0422170875660374E-4	0.0	0.0	16.64868842190615	0.0
17	1.0422170875660374E-4	0.0	0.0	17.480481879492608	0.0
18	1.0422170875660374E-4	0.0	0.0	18.59888503615972	0.0
19	1.0422170875660374E-4	0.0	0.0	19.014625432389813	1.0422170875660374E-4
20	1.0422170875660374E-4	0.0	0.0	19.516348738344103	1.0422170875660374E-4
21	1.0422170875660374E-4	0.0	0.0	20.037978390670908	1.0422170875660374E-4
22	2.084434175132075E-4	0.0	0.0	20.574824412476172	1.0422170875660374E-4
23	2.084434175132075E-4	0.0	0.0	21.112087321116466	1.0422170875660374E-4
24	3.1266512626981123E-4	0.0	0.0	21.53481057183325	1.0422170875660374E-4
25	3.1266512626981123E-4	0.0	0.0	21.92480820600046	1.0422170875660374E-4
26	3.1266512626981123E-4	0.0	0.0	22.25435724908884	1.0422170875660374E-4
27	3.1266512626981123E-4	0.0	0.0	22.63768469389563	1.0422170875660374E-4
28	3.1266512626981123E-4	0.0	0.0	23.007984425107843	1.0422170875660374E-4
29	3.1266512626981123E-4	0.0	0.0	23.380993920747727	1.0422170875660374E-4
30	3.1266512626981123E-4	0.0	0.0	23.84801139768607	1.0422170875660374E-4
31	3.1266512626981123E-4	0.0	0.0	24.2343612720468	1.0422170875660374E-4
32	3.1266512626981123E-4	0.0	0.0	24.61456206559089	1.0422170875660374E-4
33	3.1266512626981123E-4	0.0	0.0	24.99882750577649	1.0422170875660374E-4
34	3.1266512626981123E-4	0.0	0.0	25.356620631937908	1.0422170875660374E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTT	20	0.0012556652	40.0	32
ACGTTAG	25	7.7936915E-5	40.0	1
TCGGAGT	20	0.0012556652	40.0	23
CCGTACG	20	0.0012556652	40.0	1
GTTCGGA	20	0.0012556652	40.0	7
TACGACG	35	3.0556657E-7	40.0	1
TCTCGCA	20	0.0012556652	40.0	15
CGTACGG	45	1.2205419E-9	40.0	2
CGGTCGA	25	7.7936915E-5	40.0	29
TATCGTC	20	0.0012556652	40.0	13
TATCGAG	20	0.0012556652	40.0	1
CCGCAAC	20	0.0012556652	40.0	39
CGTTTTT	1840	0.0	37.5	1
CGACGGT	60	1.4551915E-11	36.666664	28
ACGCATT	225	0.0	36.444443	17
CGACAGG	165	0.0	36.363636	2
GTTAACG	55	2.1645974E-10	36.363636	1
AATCCGG	50	3.423338E-9	36.0	2
TCACCGC	230	0.0	35.652176	24
CTATGCG	45	5.440961E-8	35.555557	1
>>END_MODULE